ARL8B Antibody - #DF12350

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产品: ARL8B 抗体
货号: DF12350
描述: Rabbit polyclonal antibody to ARL8B
应用: WB IHC
文献验证: WB
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 22 kDa; 22kD(Calculated).
蛋白号: Q9NVJ2
RRID: AB_2845155

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%)
克隆:
Polyclonal
特异性:
ARL8B Antibody detects endogenous levels of total ARL8B.
RRID:
AB_2845155
引用格式: Affinity Biosciences Cat# DF12350, RRID:AB_2845155.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ADP ribosylation factor like 10C; ADP-ribosylation factor-like protein 10C; ADP-ribosylation factor-like protein 8B; ARL10C; ARL8B; ARL8B_HUMAN; FLJ10702; Gie1; Novel small G protein indispensable for equal chromosome segregation 1;

抗原和靶标

免疫原:
Uniprot:

Q9NVJ2(ARL8B_HUMAN) >>Visit HPA database.

基因/基因ID:
ARL8B(55207)
表达:
Q9NVJ2 ARL8B_HUMAN:

Ubiquitously expressed.

序列:
MLALISRLLDWFRSLFWKEEMELTLVGLQYSGKTTFVNVIASGQFSEDMIPTVGFNMRKVTKGNVTIKIWDIGGQPRFRSMWERYCRGVNAIVYMIDAADREKIEASRNELHNLLDKPQLQGIPVLVLGNKRDLPNALDEKQLIEKMNLSAIQDREICCYSISCKEKDNIDITLQWLIQHSKSRRS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Plays a role in lysosome motility. In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function (By similarity). May play a role in chromosome segregation.

细胞定位:

Late endosome membrane. Lysosome membrane. Cytoplasm>Cytoskeleton>Spindle. Cell projection>Axon. Cell junction>Synapse.
Note: Localizes with microtubules at the spindle mid-zone during mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed.

亚基结构:

Interacts with tubulin Ref.16). Interacts with BORCS5; recruits ARL8B to lysosomes.

蛋白家族:

Belongs to the small GTPase superfamily. Arf family.

文献引用

1). The role of lysosomes as intermediates in betacoronavirus PHEV egress from nerve cells. Journal of virology, 2023 (PubMed: 38009916) [IF=4.0]

Application: WB    Species: Mouse    Sample:

Fig 3 PHEV uses an Arl8b-dependent lysosomal exocytic pathway to egress. (A) Colocalization of PHEV and surface LAMP1. Mock- or PHEV-infected cells at 48 hpi were immunostained with anti-LAMP1 (red) and anti-PHEV (green) antibodies. Scale bar, 10 µm. (B) The surface LAMP1 levels on mock- and PHEV-infected cells from the experiment whose results are shown in panel A were quantified by using Image J. (C) The protein levels of Pro-CTSD, Pro-CTSB, Mat-CTSD, Mat-CTSB, and GAPDH at 24 and 48 hpi were analyzed by western blot, respectively. (D) Mock- or PHEV-infected cells at 48 hpi were immunostained with anti-LAMP1 (red), anti-Arl8b (green), and anti-PHEV (teal) antibodies. Scale bar, 10 µm. (E) The protein levels of Arl8b, PHEV, and GAPDH were analyzed by western blot in PHEV-infected Arl8b small interfering RNA (siRNA)-treated cells or PHEV-infected-non-target siRNA-treated cells. (F) The PHEV N genomic RNA was determined using qPCR in Arl8b siRNA-treated cells and non-target siRNA-treated cells. The data were normalized to the non-target siRNA-treated cells. Representative blots and images are shown. Data are shown as mean ± SD. P values were considered significant when P < 0.05 and denoted as

Application: IF/ICC    Species: Mouse    Sample:

Fig 3 PHEV uses an Arl8b-dependent lysosomal exocytic pathway to egress. (A) Colocalization of PHEV and surface LAMP1. Mock- or PHEV-infected cells at 48 hpi were immunostained with anti-LAMP1 (red) and anti-PHEV (green) antibodies. Scale bar, 10 µm. (B) The surface LAMP1 levels on mock- and PHEV-infected cells from the experiment whose results are shown in panel A were quantified by using Image J. (C) The protein levels of Pro-CTSD, Pro-CTSB, Mat-CTSD, Mat-CTSB, and GAPDH at 24 and 48 hpi were analyzed by western blot, respectively. (D) Mock- or PHEV-infected cells at 48 hpi were immunostained with anti-LAMP1 (red), anti-Arl8b (green), and anti-PHEV (teal) antibodies. Scale bar, 10 µm. (E) The protein levels of Arl8b, PHEV, and GAPDH were analyzed by western blot in PHEV-infected Arl8b small interfering RNA (siRNA)-treated cells or PHEV-infected-non-target siRNA-treated cells. (F) The PHEV N genomic RNA was determined using qPCR in Arl8b siRNA-treated cells and non-target siRNA-treated cells. The data were normalized to the non-target siRNA-treated cells. Representative blots and images are shown. Data are shown as mean ± SD. P values were considered significant when P < 0.05 and denoted as

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