(6)
(6)
产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
NR3C1 Antibody detects endogenous levels of total NR3C1.
RRID:
AB_2834793
引用格式: Affinity Biosciences Cat# AF5004, RRID:AB_2834793.
引用格式: Affinity Biosciences Cat# AF5004, RRID:AB_2834793.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
GCCR; GCR; GCR_HUMAN; glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; Glucocorticoid receptor beta isoform; GR; GRL; nr3c1; Nuclear receptor subfamily 3 group C member 1;
抗原和靶标
免疫原:
A synthesized peptide derived from human NR3C1, corresponding to a region within the internal amino acids.
基因/基因ID:
描述:
The protein encoded by this gene is a receptor for glucocorticoids and can act as both a transcription factor and a regulator of other transcription factors. The encoded protein can bind DNA as a homodimer or as a heterodimer with another protein such as the retinoid X receptor. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins.
研究领域
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
文献引用
1). Comparative Analysis of the Effects of Maternal Hypoxia and Placental Ischemia on HIF1-Dependent Metabolism and the Glucocorticoid System in the Embryonic and Newborn Rat Brain. International journal of molecular sciences, 2024
(PubMed: 39769106)
[IF=5.6]
Application: WB Species: Rat Sample: brain
Figure 4. The effect of MH and PI on the corticosterone levels (a) in the brain at e15, e16, e17, e20 and p1, detected by ELISA. The effect of MH and PI on mRNA levels of the glucocorticoid receptor (nr3c1) (b) in the brain at e15, e16, e17, e20 and p1, detected by RT PCR. The effect of MH and PI on GR protein expression levels (c) in the brain at e15, e16, e17, e20 and p1, detected by Western blotting. The effect of MH and PI on mRNA levels of the glucocorticoid-dependent genes ztb16, dusp1, and fkbp5 (d) in the brain at e15, e16, e17, e20 and p1, detected by RT PCR. (a) Corticosterone levels: e15, * p < 0.05 vs. control (one-way ANOVA, Tukey’s test); & p < 0.05 between MH and PI (one-way ANOVA, Tukey’s test). e16, * p < 0.05 vs. control (one-way ANOVA, Tukey’s test); ** p < 0.01 vs. control (one-way ANOVA, Tukey’s test). (d) mRNA levels of zbtb16: e20, * p < 0.05 vs. control (Kruskal-Wallis, Dunn’s test); ** p < 0.01 vs. control (Kruskal-Wallis, Dunn’s test). mRNA levels of dusp1: e20, * p < 0.05 vs. control (Kruskal-Wallis, Dunn’s test); ** p < 0.01 vs. control (Kruskal-Wallis, Dunn’s test). mRNA levels of fkbp5: e16, * p < 0.05 vs. control (Kruskal-Wallis, Dunn’s test); p1, * p < 0.05 vs. control (Kruskal-Wallis, Dunn’s test); && p < 0.01 between MH and PI (Kruskal-Wallis, Dunn’s test).
2). Prenatal Hypoxia Triggers a Glucocorticoid-Associated Depressive-like Phenotype in Adult Rats, Accompanied by Reduced Anxiety in Response to Stress. International journal of molecular sciences, 2024
(PubMed: 38892090)
[IF=5.6]
Application: WB Species: Rat Sample:
Figure 2. The effects of prenatal hypoxia and learned helplessness on the levels of GR and 5HT7R protein expression in the hippocampus (a), prefrontal cortex (b), amygdala (c), and on GR and CRH protein expression levels in the hypothalamus (d), and their respective representative Western blot images. (a) GR diagram: ** Welch ANOVA W (3, 7.06) = 17.66, p = 0.001; intact PSH vs. intact control, Welch’s t-test p = 0.004; * control LH vs. intact control, Welch’s t-test p = 0.02; # PSH LH vs. intact PSH, Welch’s t-test p = 0.015, n = 5. (a) 5HT7R diagram: * two-way ANOVA LH F (1, 16) = 7.833, p = 0.012; control LH vs. intact control, Fisher’s LSD test p = 0.01, n = 5. (b) GR diagram: * two-way ANOVA LH F (1, 16) = 12.14, p = 0.003; control LH vs. intact control, Fisher’s LSD test p = 0.04; # PSH LH vs. intact PSH, Fisher’s LSD test p = 0.016, n = 5. (c) GR diagram: *** two-way ANOVA LH F (1, 16) = 30.22, p < 0.0001; control LH vs. intact control, Fisher’s LSD test p = 0.0007. ** PSH LH vs. intact PSH, Fisher’s LSD test p = 0.002, n = 5. (c) 5HT7R diagram: * two-way ANOVA LH F (1, 16) = 9.8, p = 0.006; PSH LH vs. intact PSH, Fisher’s LSD test p = 0.01, n = 5. (d) CRH diagram: * Kruskal–Wallis, p = 0.004; intact control vs. intact PSH, Dunn test p = 0.009. # significant differences vs. intact PSH, intact PSH vs. PSH LH, Dunn test p = 0.009; control LH vs. intact PSH, Dunn test p = 0.008, n = 5.
3). CTNNAL1 enhances glucocorticoid sensitivity in HDM-induced asthma mouse model through deactivating hsp90 signaling pathway. LIFE SCIENCES, 2023
(PubMed: 36535402)
[IF=5.2]
4). Aucubin improves chronic unpredictable mild stress-induced depressive behavior in mice via the GR/NF-κB/NLRP3 axis. International Immunopharmacology, 2023
(PubMed: 37523973)
[IF=4.8]
5). The prenatal hypoxic pathology associated with maternal stress predisposes to dysregulated expression of the chrna7 gene and the subsequent development of nicotine addiction in adult offspring. Neuroendocrinology, 2024
(PubMed: 38198758)
[IF=3.2]
6). Effects of Chronic Restraint Stress on Apoptosis of Amygdala Cells in Rats. Fa yi xue za zhi, 2022
(PubMed: 36426688)
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