SYVN1 Antibody - #DF12235

Fig. 6. The XBP1-Hrd1 arm of the ERS pathway was triggered by high glucose. The XBP1s and Hrd1 expression in tissue samples was detected by immunohistochemical staining (n = 3) (A). Semi-quantitative analysis of XBP1s and Hrd1 expression in each group (n = 3) (B). The qRT-PCR results of Hrd1 in NC, DM, TUDCA, and PBS group (n = 3) (C). The protein expression of Hrd1 in each group of mice (n = 3) (D - E). The fluorescence intensity of XBP1s and Hrd1 in HK-2 cells was measured by the confocal fluorescence microscope. Blue represented the cell nucleus and red represented XBP1s or Hrd1 (n = 3) (F - G). Fluorescence intensity was quantified using Image J (n = 3) (H - I). The mRNA level of Hrd1 among Ctrl, HG, Mannitol, TUDCA, and DMSO groups (n = 3) (J). Immunoblot and semi-quantitative analysis of Hrd1 in each group cells (n = 3) (K - L). The qRT-PCR results of XBP1s and Hrd1 in cells transfected with empty vectors or XBP1 overexpression plasmids. At 6 h post-transfection, cells were changed to a medium containing 30 mmol/L glucose with or without TUDCA (200 μM) for 48 h (n = 3) (M). Immunoblot and quantitative analysis of XBP1s and Hrd1 under the same conditions (n = 3) (N - O). Data are expressed as the mean ± SEM.

Fig. 6. The XBP1-Hrd1 arm of the ERS pathway was triggered by high glucose. The XBP1s and Hrd1 expression in tissue samples was detected by immunohistochemical staining (n = 3) (A). Semi-quantitative analysis of XBP1s and Hrd1 expression in each group (n = 3) (B). The qRT-PCR results of Hrd1 in NC, DM, TUDCA, and PBS group (n = 3) (C). The protein expression of Hrd1 in each group of mice (n = 3) (D - E). The fluorescence intensity of XBP1s and Hrd1 in HK-2 cells was measured by the confocal fluorescence microscope. Blue represented the cell nucleus and red represented XBP1s or Hrd1 (n = 3) (F - G). Fluorescence intensity was quantified using Image J (n = 3) (H - I). The mRNA level of Hrd1 among Ctrl, HG, Mannitol, TUDCA, and DMSO groups (n = 3) (J). Immunoblot and semi-quantitative analysis of Hrd1 in each group cells (n = 3) (K - L). The qRT-PCR results of XBP1s and Hrd1 in cells transfected with empty vectors or XBP1 overexpression plasmids. At 6 h post-transfection, cells were changed to a medium containing 30 mmol/L glucose with or without TUDCA (200 μM) for 48 h (n = 3) (M). Immunoblot and quantitative analysis of XBP1s and Hrd1 under the same conditions (n = 3) (N - O). Data are expressed as the mean ± SEM.

Fig. 6. The XBP1-Hrd1 arm of the ERS pathway was triggered by high glucose. The XBP1s and Hrd1 expression in tissue samples was detected by immunohistochemical staining (n = 3) (A). Semi-quantitative analysis of XBP1s and Hrd1 expression in each group (n = 3) (B). The qRT-PCR results of Hrd1 in NC, DM, TUDCA, and PBS group (n = 3) (C). The protein expression of Hrd1 in each group of mice (n = 3) (D - E). The fluorescence intensity of XBP1s and Hrd1 in HK-2 cells was measured by the confocal fluorescence microscope. Blue represented the cell nucleus and red represented XBP1s or Hrd1 (n = 3) (F - G). Fluorescence intensity was quantified using Image J (n = 3) (H - I). The mRNA level of Hrd1 among Ctrl, HG, Mannitol, TUDCA, and DMSO groups (n = 3) (J). Immunoblot and semi-quantitative analysis of Hrd1 in each group cells (n = 3) (K - L). The qRT-PCR results of XBP1s and Hrd1 in cells transfected with empty vectors or XBP1 overexpression plasmids. At 6 h post-transfection, cells were changed to a medium containing 30 mmol/L glucose with or without TUDCA (200 μM) for 48 h (n = 3) (M). Immunoblot and quantitative analysis of XBP1s and Hrd1 under the same conditions (n = 3) (N - O). Data are expressed as the mean ± SEM.