Phospho-Smad3 (Ser208) Antibody - #AF3365

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产品: 磷酸化 Smad3 (Ser208) 抗体
货号: AF3365
描述: Rabbit polyclonal antibody to Phospho-Smad3 (Ser208)
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P84022
RRID: AB_2834780

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Smad3 (Ser208) Antibody detects endogenous levels of Smad3 only when phosphorylated at Serine 208.
RRID:
AB_2834780
引用格式: Affinity Biosciences Cat# AF3365, RRID:AB_2834780.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZP586N0721; DKFZp686J10186; hMAD 3; hMAD-3; hSMAD3; HSPC193; HST17436; JV15 2; JV15-2; JV152; LDS1C; LDS3; MAD (mothers against decapentaplegic Drosophila) homolog 3; MAD homolog 3; Mad homolog JV15 2; Mad protein homolog; MAD, mothers against decapentaplegic homolog 3; Mad3; MADH 3; MADH3; MGC60396; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD family member 3; SMAD, mothers against DPP homolog 3; Smad3; SMAD3_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human Smad3 around the phosphorylation site of Ser208.

基因/基因ID:
SMAD3(4088)
描述:
Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). Inhibition of p38 mitogen-activated protein kinases may attenuate scar proliferation after cleft lip surgery in rabbits via Smads signaling pathway. European Journal of Medical Research, 2022 (PubMed: 35858881) [IF=2.8]

Application: WB    Species: rabbit    Sample: fibroblast

Fig. 6 Effects of p38MAPK knockdown on extracellular matrix and Smads signaling pathway in fibroblast. A Effect of p38MAPK on the proliferation of scar fibroblasts was examined using the EdU assay. B mRNA expression levels of col I, col III, and α-SMA following p38MAPK knockdown as detected by RT-PCR. C Changes in protein expression of smad2, p-smad2, smad3, p-smad3, and α-SMA following p38MAPK knockdown as detected via western blotting. D Changes in protein expression of p-smad3 following p38MAPK knockdown as detected via immunofluorescence assay. Results are presented as the mean ± S.D,
2). Role of the CXCR4/ALK5/Smad3 Signaling Pathway in Cancer-Induced Bone Pain. Journal of pain research, 2020 (PubMed: 33116799) [IF=2.5]

Application: IF/ICC    Species: Rat    Sample:

Figure 1 Tumor cell implantation (TCI) increased the expression of CXCR4, ALK5 and P -Smad3 in the spinal dorsal horn of rats. (A–H) Compared with the sham-control group, expression of CXCR4, ALK5 and P-Smad3 in the rats receiving tumor cell injections (TCI) gradually increased over time; peak expression was detected at day 14 (*P < 0.05, **P < 0.01, ***P < 0.001, n = 4 in each group). (I) Increased expression was detected in the shallow layer of the dorsal horn of the spinal cord. Scale bar, 50 μm.

Application: WB    Species: Rat    Sample:

Figure 1 Tumor cell implantation (TCI) increased the expression of CXCR4, ALK5 and P -Smad3 in the spinal dorsal horn of rats. (A–H) Compared with the sham-control group, expression of CXCR4, ALK5 and P-Smad3 in the rats receiving tumor cell injections (TCI) gradually increased over time; peak expression was detected at day 14 (*P < 0.05, **P < 0.01, ***P < 0.001, n = 4 in each group). (I) Increased expression was detected in the shallow layer of the dorsal horn of the spinal cord. Scale bar, 50 μm.
3). Mechanistic insights into Y-Box binding protein-1 mediated regulation of lipid metabolism and oxidative stress in NAFLD via INHBE/TNF-β pathway. Biomolecules & biomedicine, 2024 (PubMed: 39748602)

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