产品: 磷酸化 RPS6 (Ser235) 抗体
货号: AF3354
描述: Rabbit polyclonal antibody to Phospho-RPS6 (Ser235)
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 32kDa; 29kD(Calculated).
蛋白号: P62753
RRID: AB_2834769

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-RPS6 (Ser235) Antibody detects endogenous levels of RPS6 only when phosphorylated at Serine 235.
RRID:
AB_2834769
引用格式: Affinity Biosciences Cat# AF3354, RRID:AB_2834769.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

40S ribosomal protein S6; Air8; NP33; Phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; rps6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit.
序列:
MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P62753 作为底物

Site PTM Type Enzyme
K2 Methylation
S6 Phosphorylation
C12 S-Nitrosylation
K14 Acetylation
K14 Ubiquitination
R22 Methylation
K23 Ubiquitination
Y28 Phosphorylation
K30 Acetylation
K30 Ubiquitination
K46 Ubiquitination
S53 Phosphorylation
K58 Ubiquitination
K64 Ubiquitination
T69 Phosphorylation
S78 Phosphorylation
K79 Acetylation
K79 Ubiquitination
S82 Phosphorylation
K116 Ubiquitination
K119 Ubiquitination
T128 Phosphorylation
K143 Ubiquitination
S148 Phosphorylation
K149 Ubiquitination
R154 Methylation
K160 Ubiquitination
T181 Phosphorylation
K203 Ubiquitination
Y209 Phosphorylation
K211 Acetylation
K211 Ubiquitination
K218 Acetylation
S235 Phosphorylation Q13177 (PAK2) , P51812 (RPS6KA3) , P53355 (DAPK1) , Q05655 (PRKCD) , P23443 (RPS6KB1) , Q96RG2 (PASK) , Q15418 (RPS6KA1)
S236 Phosphorylation Q05655 (PRKCD) , P23443 (RPS6KB1) , P51812 (RPS6KA3) , Q13177 (PAK2) , Q15418 (RPS6KA1) , Q96RG2 (PASK)
S240 Phosphorylation Q13177 (PAK2) , P23443 (RPS6KB1) , Q15418 (RPS6KA1)
T241 Phosphorylation
S242 Phosphorylation Q13177 (PAK2)
S244 Phosphorylation Q15418 (RPS6KA1) , P23443 (RPS6KB1)
S246 Phosphorylation
S247 Phosphorylation P23443 (RPS6KB1)

研究背景

功能:

May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

翻译修饰:

Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Specifically hydroxylated (with R stereochemistry) at C-3 of Arg-137 by KDM8.

蛋白家族:

Belongs to the eukaryotic ribosomal protein eS6 family.

研究领域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > Ribosome.

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

文献引用

1). Wang L et al. Activation of integrated stress response and disordered iron homeostasis upon combined exposure to cadmium and PCB77. JOURNAL OF HAZARDOUS MATERIALS 2019 Dec 5:121833 (PubMed: 31837937) [IF=13.6]

Application: WB    Species: Human    Sample: Human erythroleukemia cell lines (HEL)

Fig. 5. Disordered iron homeostasis and inhibited mTORC1 activity upon exposure to CdCl2 and PCB77 at low dose. (A) The relative fluorescence intensity of CAeAM for measuring LIP to reflect intracellular iron availability (n = 3–4), and (B) Representative blots of FTH1 protein content to reflect iron storage. Analyses were performed after single or combined exposure to CdCl2 and PCB77 at 1 μM for 48 h. (C) Phosphorylated S6 and total S6 content to re- flect mTORC1 activity as measured by Western blot. Ratio of FTH1 to eIF2αP and ratio of pS6 to S6 in the control group were defined as 1. Analyses were performed after single or combined exposure to CdCl2 and PCB77 at 1 μM for 48 h. a- significantly different from the control group. Data were presented in mean ± SE. P < 0.05 was considered statistically significant.

2). Li JB et al. p85S6K sustains synaptic GluA1 to ameliorate cognitive deficits in Alzheimer’s disease. Translational Neurodegeneration 2023 Jan 09;12(1) (PubMed: 36624510) [IF=12.6]

3). You Y et al. Ketogenic diet aggravates cardiac remodeling in adult spontaneously hypertensive rats. Nutrition & Metabolism 2020 Oct 26;17:91. (PubMed: 33117428) [IF=4.5]

Application: WB    Species: Rat    Sample: heart tissue

Fig. 5 Ketogenic diet activated mTORC2 in the heart of spontaneously hypertensive rats. a The phosphorylation of AMPK and the ratio of p-S6/S6 and p-Akt/Akt in the heart of Wistar rats and SHRs were measured with Western blots. The quantitative result were shown in b–d. Values are the mean ± SEM. n = 4 for each group. *P < 0.05, **P < 0.01

4). Zhang JQ et al. Autophagy Contributes to Oxidative Stress-Induced Apoptosis in Porcine Granulosa Cells. Reproductive Sciences 2020 Oct 20. (PubMed: 33079330) [IF=2.9]

Application: WB    Species: Porcine    Sample: granulosa cell (GC)

Fig. 5 H2O2 induces GC autophagy through the mTOR and Ras-cAMPPKA signaling pathway. Primary cultured GC were treated with H2O2 (200 μmol/l) for different time periods. Then, the GC samples were analyzed by immunoblotting using the corresponding antibody. a The expression of mTOR protein was determined by western blotting. b The expression of PI3K, AKT, and RPS6 was determined by western blotting c The expression of Ras, cAMP, and PKA was determined by western blotting. d–j The relative expression of p-mTOR, p-PI3K, p-AKT, pRPS6, Ras, cAMP, and PKA were quantified by software Image J. Data represent as the means ± SE from three independent experiments. * p < 0.05, ** p < 0.01

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