SREBP1 Antibody - #AF4728

Figure 3. PCA promotes fatty acid metabolism through SIRT3 activation. (A-E) Western blotting and real-time PCR analysis of hepatic FASN, SREBP-1c, CPT1, Acox1 and PPARα protein (n=5) and mRNA (n=6) levels in mouse livers. *P< 0.05 vs. the WT ND group, #P< 0.05 vs. the WT HFD group, &P< 0.05 vs. the SIRT3−/− ND group. (F-I) The protein expression of FASN, SREBP-1c, CPT1, Acox1, PPARα and SIRT3 was measured by Western blotting (n=5). The mRNA levels of (J) FASN, SREBP-1c (K) CPT1, Acox1 and PPARα were measured with real-time PCR (n=6). *P< 0.05 vs. the si-Control group, #P< 0.05 vs. the si-Control+PA group, &P< 0.05 vs. the si-Control+PA+PCA group.

Figure 3. Effects of AMC on the protein levels of AMPKα-SREBP-1c/PPARα-regulating hepatic lipid metabolism in transported rats. (A–D) The protein levels of phosphorylated AMP-activated protein kinase alpha (p-AMPKα)/AMPKα, AMP-activated protein kinase alpha (AMPKα), AMPKα1, AMPKα2, sterol regulatory element-binding protein-1c (SREBP-1c), and Peroxisome proliferator-activated receptor alpha (PPARα) in the liver (A) on days −3 and 0, (B) at IAT, (C) on day 3, and (D) on day 10. Rats were assigned to one of six groups: Con, TS, ABT, VBT, AAT, and VAT groups. Data are expressed as the mean ± standard error of the mean (n = 6). * mean Con group on day −3 vs. day 0; Con vs. ABT or VBT on day 0; TS vs. Con, ABT, or VBT at IAT; and TS vs. Con, ABT, VBT, AAT, or VAT on days 3 and 10; # (black) mean ABT vs. VBT on days 0, 3, and 10 and at IAT, and AAT vs. VAT on days 3 and 10; # (red) mean ABT vs. AAT and VBT vs. VAT on days 3 and 10. *, # (black), and # (red): p < 0.05; **, ## (black), and ## (red): p < 0.01.

Fig. 2. Siah1 overexpression exacerbates HFD-induced lipid accumulation. A. Biochemical markers of liver function in the serum of Ad-Siah1 and Ad-GFP mice fed with 60% HFD for 16 weeks. B. Representative images of macroscopic examination, HE (original magnification, ×100) and oil red O (original magnification, ×400) staining of mice liver tissue obtained from Ad-Siah1 and Ad-GFP mice fed with 60% HFD for 16 weeks. C. Changes in liver weights. D. Spectrometer quantification of oil red O staining. E-F. Relative protein and mRNA expression levels of Siah1 and lipid metabolic genes (SREBP1, FASN and ACC1) in mice liver tissue obtained from Ad-Siah1 and Ad-GFP mice fed with 60% HFD for 16 weeks. G-H. Lipid accumulations displayed by oil red O staining (original magnification, ×200) and spectrometer quantification in Hepa1-6/Ad-Siah1 or AML12/Ad-Siah1 cells after 24 h OA/PA treatment. I-J. Relative protein and mRNA expression levels of Siah1 and lipid metabolic genes (SREBP1, FASN and ACC1) in Hepa1-6/Ad-Siah1 or (K-L) AML12/Ad-Siah1 cells after 24 h OA/PA treatment. The data are presented as mean ± SD. Student’s t-test was used to evaluate the statistical significance. *P < 0.05, **P < 0.01 vs. Ad-GFP.

Figure 5 Effects of Si-Ni-San (SNS) on the protein expressions related to lipid metabolism. The protein expressions of FAS, CPT-1, nuclear and cytosolic SREBP-1c and PPARα were determined by Western blot. (A and B) Typical bands of Western blot, (C–F) gray value of Western blot bands. The data are stated as the means ± SD. n = 3 *vs CON group (P < 0.05), #vs MOD group (P < 0.05). Liver was obtained from control group (CON), model group (MOD), low-dose SNS group (SNS-L), high-dose SNS group (SNS-H), and metformin group (MET).

Fig. 2 Validation of the predicted genes in ox-LDL-induced mouse macrophages. A, circSnd1, miR-485-3p, and Olr1 mRNA expression verification. B, Olr1 protein expression verification. C, Verification of the expression changes in inflammatory signaling molecules possibly downstream of the circSnd1/miR-485-3p/Olr1 axis in mouse macrophages after treatment with ox-LDL. D, Changes in the expression of key proteins in lipid metabolism in ox-LDL-induced mouse macrophages. *, #p < 0.05; **, ##p < 0.01.

Figure 7 The expression levels of lipid metabolism-related proteins after chronic lactate and forskolin injection. (A) Western blot analysis and relative fold protein expression of lipolysis-related proteins. (B) Western blot analysis and relative fold protein expression of lipogenesis-related proteins. CP, chronic PBS treated group; CL, chronic lactate treated group; CF, chronic forskolin treated group; CD, chronic DMSO treated group; and CLF, chronic lactate and forskolin treated group. Relative expression levels were normalized to GAPDH. Three bands are used for statistics. The data are presented as the mean±SD, and significant differences among the groups were analyzed with one-way ANOVA. * p<0.05.

Fig. 1 Effect of taurine on the TG level and FA synthesis-related enzymes and factors protein level in HepG2 cells. (a) TG levels; (b, d) protein band diagram; (c, e) Relative protein level. C: control group; CT: control supplemented with taurine; M: steatotic cell model; MT: steatotic model supplemented with taurine. ∗p < 0.05 vs. C group; #p < 0.05 vs. M group.