产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3334, RRID:AB_2834749.
展开/折叠
FLJ43106; LAM; OTTHUMP00000158940; OTTHUMP00000198394; OTTHUMP00000198395; PPP1R160; Protein phosphatase 1, regulatory subunit 160; tsc2; TSC2_HUMAN; TSC4; TSC4 gene, formerly; TSC4, formerly; Tuberin; Tuberous sclerosis 2; Tuberous sclerosis 2 protein; Tuberous sclerosis 2 protein homolog;
抗原和靶标
Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
- P49815 TSC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P49815 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K3 | Ubiquitination | Uniprot | |
K7 | Acetylation | Uniprot | |
K7 | Ubiquitination | Uniprot | |
K12 | Acetylation | Uniprot | |
K16 | Ubiquitination | Uniprot | |
T23 | Phosphorylation | Uniprot | |
K34 | Ubiquitination | Uniprot | |
K69 | Acetylation | Uniprot | |
K69 | Ubiquitination | Uniprot | |
K72 | Ubiquitination | Uniprot | |
K106 | Ubiquitination | Uniprot | |
K128 | Ubiquitination | Uniprot | |
K144 | Ubiquitination | Uniprot | |
S188 | Phosphorylation | Uniprot | |
K250 | Ubiquitination | Uniprot | |
K258 | Ubiquitination | Uniprot | |
K438 | Ubiquitination | Uniprot | |
S540 | Phosphorylation | Uniprot | |
K634 | Ubiquitination | Uniprot | |
K658 | Ubiquitination | Uniprot | |
S664 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
K704 | Ubiquitination | Uniprot | |
R751 | Methylation | Uniprot | |
T760 | Phosphorylation | Uniprot | |
K914 | Ubiquitination | Uniprot | |
S923 | Phosphorylation | Uniprot | |
T927 | Phosphorylation | Uniprot | |
K930 | Ubiquitination | Uniprot | |
S932 | Phosphorylation | Uniprot | |
S937 | Phosphorylation | Uniprot | |
T938 | Phosphorylation | Uniprot | |
S939 | Phosphorylation | P31749 (AKT1) , P31751 (AKT2) | Uniprot |
S946 | Phosphorylation | Uniprot | |
K954 | Ubiquitination | Uniprot | |
S960 | Phosphorylation | Uniprot | |
K964 | Ubiquitination | Uniprot | |
K967 | Methylation | Uniprot | |
S981 | Phosphorylation | P31749 (AKT1) | Uniprot |
S999 | Phosphorylation | Uniprot | |
K1043 | Ubiquitination | Uniprot | |
K1058 | Ubiquitination | Uniprot | |
S1096 | Phosphorylation | Uniprot | |
S1097 | Phosphorylation | Uniprot | |
K1106 | Ubiquitination | Uniprot | |
K1111 | Ubiquitination | Uniprot | |
R1122 | Methylation | Uniprot | |
S1130 | Phosphorylation | Uniprot | |
S1132 | Phosphorylation | Uniprot | |
S1155 | Phosphorylation | Uniprot | |
K1168 | Ubiquitination | Uniprot | |
K1180 | Ubiquitination | Uniprot | |
Y1186 | Phosphorylation | Uniprot | |
T1203 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
K1242 | Ubiquitination | Uniprot | |
Y1250 | Phosphorylation | Uniprot | |
K1251 | Ubiquitination | Uniprot | |
S1252 | Phosphorylation | Uniprot | |
S1254 | Phosphorylation | P49137 (MAPKAPK2) | Uniprot |
K1262 | Ubiquitination | Uniprot | |
S1269 | Phosphorylation | Uniprot | |
T1271 | Phosphorylation | Uniprot | |
S1304 | Phosphorylation | Uniprot | |
S1334 | Phosphorylation | Uniprot | |
S1337 | Phosphorylation | Uniprot | |
S1338 | Phosphorylation | Uniprot | |
S1340 | Phosphorylation | Uniprot | |
S1341 | Phosphorylation | Uniprot | |
S1346 | Phosphorylation | Uniprot | |
R1355 | Methylation | Uniprot | |
S1364 | Phosphorylation | Uniprot | |
S1365 | Phosphorylation | Uniprot | |
S1371 | Phosphorylation | Uniprot | |
S1375 | Phosphorylation | Uniprot | |
S1379 | Phosphorylation | Uniprot | |
S1383 | Phosphorylation | Uniprot | |
S1385 | Phosphorylation | Uniprot | |
S1386 | Phosphorylation | Uniprot | |
S1387 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
S1388 | Phosphorylation | Uniprot | |
T1393 | Phosphorylation | Uniprot | |
S1411 | Phosphorylation | Uniprot | |
S1418 | Phosphorylation | Uniprot | |
S1420 | Phosphorylation | Uniprot | |
T1422 | Phosphorylation | Uniprot | |
S1427 | Phosphorylation | Uniprot | |
S1448 | Phosphorylation | Uniprot | |
S1449 | Phosphorylation | Uniprot | |
S1452 | Phosphorylation | Uniprot | |
S1454 | Phosphorylation | Uniprot | |
Y1461 | Phosphorylation | Uniprot | |
T1462 | Phosphorylation | P31749 (AKT1) , P31751 (AKT2) | Uniprot |
S1466 | Phosphorylation | Uniprot | |
S1469 | Phosphorylation | Uniprot | |
Y1571 | Phosphorylation | Uniprot | |
K1585 | Ubiquitination | Uniprot | |
S1647 | Phosphorylation | Uniprot | |
Y1650 | Phosphorylation | Uniprot | |
S1653 | Phosphorylation | Uniprot | |
K1658 | Ubiquitination | Uniprot | |
S1684 | Phosphorylation | Uniprot | |
K1689 | Ubiquitination | Uniprot | |
T1697 | Phosphorylation | Uniprot | |
K1701 | Ubiquitination | Uniprot | |
S1704 | Phosphorylation | Uniprot | |
S1730 | Phosphorylation | Uniprot | |
Y1736 | Phosphorylation | Uniprot | |
K1739 | Ubiquitination | Uniprot | |
Y1760 | Phosphorylation | Uniprot | |
S1761 | Phosphorylation | Uniprot | |
S1764 | Phosphorylation | Uniprot | |
K1775 | Ubiquitination | Uniprot | |
T1780 | Phosphorylation | Uniprot | |
Y1788 | Phosphorylation | Uniprot | |
S1798 | Phosphorylation | Q15418 (RPS6KA1) , P51812 (RPS6KA3) , Q9P1W9 (PIM2) | Uniprot |
S1799 | Phosphorylation | Uniprot |
研究背景
In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. May also play a role in microtubule-mediated protein transport (By similarity). Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity).
Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1. Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation. Phosphorylation by AMPK activates it and leads to negatively regulates the mTORC1 complex. Phosphorylated at Ser-1798 by RPS6KA1; phosphorylation inhibits TSC2 ability to suppress mTORC1 signaling. Phosphorylated by DAPK1.
Ubiquitinated by the DCX(FBXW5) E3 ubiquitin-protein ligase complex, leading to its subsequent degradation. Ubiquitinated by MYCBP2 independently of its phosphorylation status leading to subsequent degradation; association with TSC1 protects from ubiquitination.
Cytoplasm. Membrane>Peripheral membrane protein.
Note: At steady state found in association with membranes.
Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones CDC37, PPP5C, TSC1 and client protein TSC2, CDK4, AKT, RAF1 and NR3C1; this complex does not contain co-chaperones STIP1/HOP and PTGES3/p23. Forms a complex containing HSP90AA1, TSC1 and TSC2; TSC1 is required to recruit TCS2 to the complex thereby stabilizing TSC2. Interacts with TSC1 and HERC1; the interaction with TSC1 stabilizes TSC2 and prevents the interaction with HERC1. May also interact with the adapter molecule RABEP1. The final complex may contain TSC2 and RABEP1 linked to RAB5. Interacts with HSPA1 and HSPA8. Interacts with DAPK1. Interacts with FBXW5. Interacts with NAA10 (via C-terminal domain). Interacts with RRAGA (polyubiquitinated). Interacts with WDR45B. Interacts with RPAP3 and URI1.
(Microbial infection) Interacts with human cytomegalovirus protein UL38; this interaction inhibits cellular stress response mediated by mTORC1.
研究领域
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
文献引用
Application: WB Species: Human Sample: BT474 cell
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