Phospho-STAT1 (Tyr701) Antibody - #AF3300

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产品: 磷酸化 STAT1 (Tyr701) 抗体
货号: AF3300
描述: Rabbit polyclonal antibody to Phospho-STAT1 (Tyr701)
应用: WB IHC IF/ICC IP
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
蛋白号: P42224
RRID: AB_2834719

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WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-STAT1 (Tyr701) Antibody detects endogenous levels of STAT1 only when phosphorylated at Tyrosine 701.
RRID:
AB_2834719
引用格式: Affinity Biosciences Cat# AF3300, RRID:AB_2834719.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CANDF7; DKFZp686B04100; ISGF 3; ISGF3; OTTHUMP00000163552; OTTHUMP00000165046; OTTHUMP00000165047; OTTHUMP00000205845; Signal transducer and activator of transcription 1; Signal transducer and activator of transcription 1, 91kDa; Signal transducer and activator of transcription 1-alpha/beta; Stat1; STAT1_HUMAN; STAT91; Transcription factor ISGF-3 components p91/p84;

抗原和靶标

免疫原:

A synthesized peptide derived from human STAT1 around the phosphorylation site of Tyr701.

基因/基因ID:
STAT1(6772)
描述:
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

文献引用

1). Calcium silicate-human serum albumin composite hydrogel decreases random pattern skin flap necrosis by attenuating vascular endothelial cell apoptosis and inflammation. Chemical Engineering Journal, 2021 [IF=13.3]
2). A Constant Filgotinib Delivery Adhesive Platform Based on Polyethylene Glycol (PEG) Hydrogel for Accelerating Wound Healing via Restoring Macrophage Mitochondrial Homeostasis. Small (Weinheim an der Bergstrasse, Germany), 2024 (PubMed: 39679768) [IF=13.0]
3). Targeting CDCP1 boost CD8+ T cells-mediated cytotoxicity in cervical cancer via the JAK/STAT signaling pathway. Journal for immunotherapy of cancer, 2024 (PubMed: 39455095) [IF=10.9]
4). Resveratrol-loaded sulfated Hericium erinaceus β-glucan-chitosan nanoparticles: Preparation, characterization and synergistic anti-inflammatory effects. Carbohydrate polymers, 2024 (PubMed: 38431417) [IF=10.7]
5). Chemical structure and anti-inflammatory activity of a branched polysaccharide isolated from Phellinus baumii. Carbohydrate Polymers, 2021 (PubMed: 34127216) [IF=10.7]

Application: WB    Species: Mouse    Sample: RAW 264.7 cells

Fig. 5. |Effects of SHPS-1 on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The internal panels represent the effect of SHPS-1 and LPS on (A) cell proliferation, (B) nitric oxide concentration in cell culture medium, (C) gene expression, (D) proteins levels analyzed by western blot.

Application: IF/ICC    Species: Mouse    Sample: RAW 264.7 cells

Fig. 5. |Effects of SHPS-1 on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The internal panels represent the effect of SHPS-1 and LPS on (A) cell proliferation, (B) nitric oxide concentration in cell culture medium, (C) gene expression, (D) proteins levels analyzed by western blot.(E) NF-KB p65 nucleus translocation analyzed by immunofluorescence (IF), (F) phosphorylation level of STAT1 analyzed by IF
6). Aligned electrospun poly(l-lactide) nanofibers facilitate wound healing by inhibiting macrophage M1 polarization via the JAK-STAT and NF-κB pathways. JOURNAL OF NANOBIOTECHNOLOGY, 2022 (PubMed: 35883095) [IF=10.2]

Application: WB    Species: Mice    Sample:

Fig. 3 The underlying mechanism by which aligned fibers affected macrophage polarization. A Venn diagram showing differentially expressed genes. B KEGG pathway analysis between the A20 and R20 groups. C Heatmap of differentially expressed genes among the three groups. D Heatmap of macrophage polarization-related genes between the A20 and R20 groups. E Volcano diagram of differentially expressed genes. F Western blot analysis of the NF-κB signaling pathway. G Immunofluorescence staining showing the nuclear translocation of NF-κB p65. The nucleus is stained blue, and NF-κB p65 protein is stained red. H Western blot images and semiquantitative analysis of the JAK-STAT signaling pathway (*p < 0.05, **p < 0.01, n = 3)
7). IFN-γ signaling links ventriculomegaly to choroid plexus and ependyma dysfunction following maternal immune activation. Journal of neuroinflammation, 2025 (PubMed: 40089736) [IF=9.3]
8). Tofacitinib Promotes Functional Recovery after Spinal Cord Injury by Regulating Microglial Polarization via JAK/STAT Signaling Pathway. International Journal of Biological Sciences, 2023 (PubMed: 37781508) [IF=8.2]

Application: WB    Species: Rat    Sample: spinal cord

Figure 8 TOF normalizes the activated STAT signaling in injured spinal cord and ex vivo microglia under inflammatory conditions. (A) Western blot analysis performed for p-STAT1, STAT1, p-STAT3 and STAT3 expression in spinal cord obtained on day 7 post-injury. (B) Western blot analysis of p-STAT1, STAT1, p-STAT3 and STAT3 expression in primary microglia. (C) Quantification of the ratio of p-STAT1/ STAT1 and p-STAT3/ STAT3 (The values are presented as mean ± SD; *p
9). FAM210B activates STAT1/IRF9/IFIT3 axis by upregulating IFN-α/β expression to impede the progression of lung adenocarcinoma. Cell Death & Disease, 2025 [IF=8.1]
10). FAM210B activates STAT1/IRF9/IFIT3 axis by upregulating IFN-α/β expression to impede the progression of lung adenocarcinoma. Cell death & disease, 2025 (PubMed: 39900908) [IF=8.1]
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