Phospho-STAT3 (Ser727) Antibody - #AF3294

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产品: 磷酸化 STAT3 (Ser727) 抗体
货号: AF3294
描述: Rabbit polyclonal antibody to Phospho-STAT3 (Ser727)
应用: WB IHC IF/ICC IP
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
蛋白号: P40763
RRID: AB_2834713

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, IP 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-STAT3 (Ser727) Antibody detects endogenous levels of STAT3 only when phosphorylated at Serine 727.
RRID:
AB_2834713
引用格式: Affinity Biosciences Cat# AF3294, RRID:AB_2834713.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

1110034C02Rik; Acute Phase Response Factor; Acute-phase response factor; ADMIO; APRF; AW109958; DNA binding protein APRF; FLJ20882; HIES; MGC16063; Signal transducer and activator of transcription 3 (acute phase response factor); Signal transducer and activator of transcription 3; STAT 3; Stat3; STAT3_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human STAT3 around the phosphorylation site of Ser727.

基因/基因ID:
STAT3(6774)
描述:
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Mesenchymal Stem Cells Overexpressing ACE2 Favorably Ameliorate LPS-Induced Inflammatory Injury in Mammary Epithelial Cells. Frontiers in Immunology, 2022 (PubMed: 35095873) [IF=5.7]

Application: WB    Species: Mice    Sample: EpH4-Ev cells

Figure 7 MSC-ACE2 upregulates IL-10/STAT3/SOCS3 signaling pathway expression to suppress LPS-induced inflammation in EpH4-Ev cells. (A) Detection of relative protein expression levels of IL-10, phosphorylation levels of STAT3, STAT3 and SOCS3 by Western blot; (B–D), Statistics of the IL-10, phosphorylation levels of STAT3, STAT3 and SOCS3 Western blot results. Experiments were repeated three times and data were presented as the mean ± SEM (n = 4). * P < 0.05 vs. EpH4-Ev; # P < 0.05 vs. LPS; $ P < 0.05 vs. MSC; & P < 0.05 vs. MSC-GFP.
2). Threonine Deficiency Increases Triglyceride Deposition in Primary Duck Hepatocytes by Reducing STAT3 Phosphorylation. International journal of molecular sciences, 2024 (PubMed: 39125712) [IF=5.6]
3). Gondoic acid alleviates LPS‑induced Kupffer cells inflammation by inhibiting ROS production and PKCθ/ERK/STAT3 signaling pathway. International Immunopharmacology, 2022 (PubMed: 35998508) [IF=4.8]
4). Qing-Luo-Yin Alleviated Experimental Arthritis in Rats by Disrupting Immune Feedback Between Inflammatory T Cells and Monocytes: Key Evidences from Its Effects on Immune Cell Phenotypes. Journal of Inflammation Research, 2023 (PubMed: 35002280) [IF=4.5]

Application: WB    Species: Rat    Sample:

Figure 6 Effects of QLY-related anti-rheumatic compounds on AIA lymphocytes in vitro. (A) Levels of IFN-γ and IL-17α in the medium released by compounds-treated AIA lymphocytes, determined by ELISA; (B) expression of protein (p)-STAT3 in compounds-treated AIA lymphocytes, assessed by Western blot analysis; (C) quantification of assay B; (D) mRNA expression of IL-4, SIRT1, IFN-γ and IL-17α in compounds-treated AIA lymphocytes, evaluated by RT-qPCR. Statistical significance: *p < 0.05 and **p < 0.01 compared with untreated AIA lymphocytes; #p < 0.05 and ##p < 0.01 compared with AIA lymphocytes receiving combination stimulus (SIN + SCA + MT). Abbreviations: QLY, Qing-Luo-Yin; AIA, adjuvant-induced arthritis; SIN, sinomenine; SCA, sophocarpine; MT, matrine.
5). Umbilical cord‑derived mesenchymal stem cells can inhibit the biological functions of melanoma A375 cells. ONCOLOGY REPORTS, 2018 (PubMed: 29767256) [IF=3.8]
6). LYPD8 regulates the proliferation and migration of colorectal cancer cells through inhibiting the secretion of IL‑6 and TNF‑α. Oncology Reports, 2019 (PubMed: 30816524) [IF=3.8]
7). Cyclin G2 inhibits oral squamous cell carcinoma growth and metastasis by binding to IGFBP3 and regulating the FAK-SRC-STAT signaling pathway. Frontiers in Oncology, 2020 (PubMed: 33240810) [IF=3.5]

Application: WB    Species: Human    Sample: SCC-9 cells

Figure 5 Cyclin G2 inhibits the FAK-SRC-STAT pathway in vitro and in vivo. (A) The protein expressions along with quantification of FAK, P-FAK, SRC, P-SRC, STAT3, P-STAT3, Bcl-2, c-Myc, and MMP9 in the FAK-SRC-STAT pathway. (B–D) The relative mRNA expressions of Bcl-2, c-Myc and MMP9. (E) Analysis of MMP9 secretion and its quantification in SCC-9 cells. (F, G) Immunohistochemical staining and analysis of the expression of cyclin G2, p-FAK, p-SRC and p-STAT3. Scale bar = 100 µm. *p < 0.05, **p < 0.01, ***p < 0.001 vs. vector.
8). High expression of malic enzyme 1 predicts adverse prognosis in patients with cytogenetically normal acute myeloid leukaemia and promotes leukaemogenesis through the IL-6/JAK2/STAT3 pathways. Therapeutic advances in hematology, 2024 (PubMed: 39610460) [IF=3.4]
9). STAT3/miR-15a-5p/CX3CL1 Loop Regulates Proliferation and Migration of Vascular Endothelial Cells in Atherosclerosis. International Journal of Medical Sciences, 2021 (PubMed: 33456354) [IF=3.2]

Application: WB    Species: Human    Sample: HUVECs

Figure 2 CX3CL1 expression activated in HUVECs by STAT3 signaling pathway. A-D: The effect of treated with IL-6 on CX3CL1 expression in HUVECs was detected by western blot analysis and qRT-PCR. Cells were stimulated by IL-6 at various concentrations (0, 10, 20, 40 60 and 80, 100 ng/mL) for 24 hours or at different times (0, 6, 12, 24, and 48h) at a concentration of 80ng/mL. All experiments were repeated three times in duplicate, and the semi-quantitative analysis was calculated by SPSS statistical soft. (**P< 0.01). E and F: The effect of treated with overexpression STAT3 or knocked down STAT3 on the expression of CX3CL1 was examined by western blot analysis and qRT-PCR in HUVECs. All experiments were repeated three times in duplicate, and the semi-quantitative analysis was calculated by SPSS statistical soft. (**P< 0.01)
10). Biological functions of lung cancer cells are suppressed in co-culture with mesenchymal stem cells isolated from umbilical cord. Experimental and Therapeutic Medicine, 2018 (PubMed: 29399109) [IF=2.4]
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