RIPK1 Antibody - #AF7877

Figure 2. |FnEVs promote TNF-α induced Caco2 cells death and barrier loss. (a) Diagram of groups. (b) Apoptosis was analyzed by using the annexin V FITC/PI assay. (c) Bar graphs show the relative mean of Caco2 cells apoptosis rate in different view fields. (d) The releasing levels of LDH were detected. (e) Representative images of TUNEL stainings of Caco-2 cells (green, TUNEL positive; blue, DAPI). Scale bar = 50 um. (f) Immunoblot analysis of protein extracts from Caco2 cells with the indicated antibodies

Figure 6. |RIPK1 mediated epithelial cell death drives exacerbated barrier loss in FnEVs-treated colitis mice. (a) Representative images of TUNEL stainings of colon sections on day 3 after colitis induction (red, TUNEL positive; blue, DAPI). Scale bar = 50 um (up) or 20 um (down). (b) Representative images of immunohistochemical stainings of FADD, RIPK1 and cCASP3 in the colon sections on day 3 after colitis induction. Scale bar = 50 um or 20 um (downmost).

Fig. 2 TI ameliorated t‑BHP induced cell necroptosis via RIP1/RIP3/MLKL pathway. a H9c2 cells were cultured with Nec-1 for 12 h. b Cells were exposed to t-BHP (150 μM) for 10 h after treated with Nec-1 for 2 h. c Cells were exposed to t-BHP (150 μM) for 10 h after treated with TI (1 μM) or Nec-1 (100 μM) for 2 h. MTT was employed to detect cell viability. d Cells were treated with t-BHP (150 μM) for 10 h when pretreated with TI (1 μM) or Nec-1 (100 μM) for 2 h, the LDH level was monitored by LDH kit. e–h Pretreated with TI (0.25, 0.5, and 1 μM) or Nec-1 (100 μM) for 2 h respectively, then exposed to t-BHP (150 μM) for 4 h, the protein expression was determined by western blotting. n = 3. *p < 0.05, **p < 0.01, ***p < 0.001 vs. t-BHP group

FIGURE 2 | Irradiation-induced cellular apoptosis in liver tissues. (A and B) Irradiated liver tissues were collected at days 1 and 3 after TBI. Representative pictures by TUNEL staining are shown (A, n = 6). Scale bar = 50 μm. Arrows indicate TUNEL-positive cells. The ratios of apoptotic cells in each field are presented in (B) (N.D: non-detected). (C and D) RIPK1 expression in liver tissues (n = 3). Expression of RIPK1 in liver tissues was detected by western blotting (C). GAPDH was used as a housekeeping control. Expression of RIPK1 was quantitated by ImageJ software (D).