mTOR Antibody - #AF7803

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产品: mTOR 抗体
货号: AF7803
描述: Rabbit polyclonal antibody to mTOR
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: P42345
RRID: AB_2844167

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
mTOR Antibody detects endogenous levels of total mTOR.
RRID:
AB_2844167
引用格式: Affinity Biosciences Cat# AF7803, RRID:AB_2844167.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1); FK506 binding protein 12 rapamycin associated protein 1; FK506 binding protein 12 rapamycin associated protein 2; FK506 binding protein 12 rapamycin complex associated protein 1; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP rapamycin associated protein; FKBP12 rapamycin complex associated protein; FKBP12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FLJ44809; FRAP; FRAP1; FRAP2; Mammalian target of rapamycin; Mechanistic target of rapamycin; mTOR; MTOR_HUMAN; OTTHUMP00000001983; RAFT1; Rapamycin and FKBP12 target 1; Rapamycin associated protein FRAP2; Rapamycin target protein 1; Rapamycin target protein; RAPT1; Serine/threonine-protein kinase mTOR;

抗原和靶标

免疫原:

A synthesized peptide derived from human mTOR, corresponding to a region within C-terminal amino acids.

基因/基因ID:
MTOR(2475)

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - other.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Regulation of the PTEN/PI3K/AKT pathway in RCC using the active compounds of natural products in vitro. Molecular Medicine Reports, 2021 (PubMed: 34490473) [IF=3.4]

Application: WB    Species: Human    Sample: 786-O and OS-RC-2 cells

Figure 5. Naringenin inhibits renal cell carcinoma progression via activation of the PTEN/PI3K/AKT signaling pathway. Western blot analysis of PTEN, mTOR, p-mTOR, PI3K, AKT and p-AKT expression in 786-O and OS-RC-2 cells. Data are presented as the mean ± SD. *P<0.05, **P<0.01 vs. Abs. Abs, absolute ethanol; p-, phosphorylated.

Application: WB    Species: human    Sample: 786‑O and OS‑RC‑2 cells

Figure 5.| Naringenin inhibits renal cell carcinoma progression via activation of the PTEN/PI3K/AKT signaling pathway. Western blot analysis of PTEN,mTOR, p‑mTOR, PI3K, AKT and p‑AKT expression in 786‑O and OS‑RC‑2 cells. Data are presented as the mean ± SD. *P<0.05, **P<0.01 vs. Abs. Abs, abso‑lute ethanol; p‑, phosphorylated.
2). The miR‑136‑5p/ROCK1 axis suppresses invasion and migration, and enhances cisplatin sensitivity in head and neck cancer cells. Experimental and Therapeutic Medicine, 2021 (PubMed: 33717260) [IF=2.4]

Application: WB    Species: Human    Sample: laryngeal squamous cell carcinoma and head and neck squamous cell carcinoma cells

Figure 4 miR-136-5p overexpression increases the cisplatin sensitivity of laryngeal squamous cell carcinoma and head and neck squamous cell carcinoma cells. (A) After cells were transfected with miR-136-5p mimics or mimics-NC and treated with cisplatin (2.6 µM) for 24 h, cell viability was detected using MTT assays, n=6. (B) Flow cytometric detection of apoptosis using annexin V-FITC/PI staining and (C) quantification of apoptosis. Autophagy was detected using an autophagy double-labeled adenovirus infection assay in (D) FD-LSC-1 and (E) FaDu cells, scale bar=50 µm. Free yellow puncta indicates the autophagosome, free red puncta indicates the autophagolysosome. (F) The protein expression levels of ROCK1, p-Akt/Akt (Ser473), GSK-3β, p-GSK-3β (Ser9), p-mTOR (S2448)/mTOR, LC3II/I and P62 were measured using western blot analysis and normalized to the levels of β-actin. Data are presented as the mean ± SD, n=3. *P<0.05. GSK-3β, glycogen synthase kinase-3β; LC3, microtubule-associated protein 1 light chain 3; miR, microRNA; mTOR, mammalian target of rapamycin; NC, negative control; p-, phosphorylated.

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