PKA alpha/beta/gamma CAT Antibody - #AF7746

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产品: PKA alpha/beta/gamma CAT 抗体
货号: AF7746
描述: Rabbit polyclonal antibody to PKA alpha/beta/gamma CAT
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Dog, Xenopus
蛋白号: P17612 | P22694 | P22612
RRID: AB_2844110

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
PKA alpha/beta/gamma CAT Antibody detects endogenous levels of total PKA alpha/beta/gamma CAT.
RRID:
AB_2844110
引用格式: Affinity Biosciences Cat# AF7746, RRID:AB_2844110.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

cAMP dependent protein kinase alpha catalytic subunit; cAMP dependent protein kinase beta catalytic subunit; cAMP dependent protein kinase catalytic beta subunit isoform 4ab; cAMP dependent protein kinase catalytic subunit alpha; cAMP dependent protein kinase catalytic subunit alpha, isoform 1; cAMP dependent protein kinase catalytic subunit beta; cAMP-dependent protein kinase catalytic subunit alpha; KAPCA_HUMAN; PKA C alpha; PKA C beta; PKA C-alpha; PKACA; PKACB; PPNAD4; PRKACA; PRKACAA; PRKACB; Protein kinase A catalytic subunit alpha; Protein kinase A catalytic subunit; Protein kinase A catalytic subunit beta; Protein kinase, cAMP dependent, catalytic, alpha; Protein kinase, cAMP dependent, catalytic, beta; cAMP-dependent protein kinase catalytic beta subunit isoform 4ab; cAMP-dependent protein kinase catalytic subunit beta; KAPCB_HUMAN; PKA C beta; PKA C-beta; PKACB; Prkacb; protein kinase A catalytic subunit beta; Protein kinase cAMP dependent catalytic beta; cAMP-dependent protein kinase catalytic subunit gamma; KAPCG_HUMAN; KAPG; PKA C gamma; PKA C-gamma; PKACg; PRKACG; Protein kinase cAMP dependent catalytic gamma; Serine (threonine) protein kinase;

抗原和靶标

免疫原:

A synthesized peptide derived from human PKA alpha/beta/gamma CAT, corresponding to a region within the internal amino acids.

基因/基因ID:
PRKACG(5568) | PRKACA(5566) | PRKACB(5567)

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Morphine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > GABAergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Sensory system > Taste transduction.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

文献引用

1). Beta-Cell Tipe1 Orchestrates Insulin Secretion and Cell Proliferation by Promoting Gαs/cAMP Signaling via USP5. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 38417114) [IF=15.1]

Application: WB    Species: Mouse    Sample: MIN6 cells

Figure 3 Tipe1 maintains β cell function via Gαs/cAMP pathway. A) Tipe1 interaction proteins in MIN6 cells and differentially expressed genes in human IGT and T2D (Hba1c > 6%, 0.2 < logFC
2). β-asarone improves cognitive impairment and alleviates autophagy in mice with vascular dementia via the cAMP/PKA/CREB pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38039902) [IF=6.7]
3). Noncoding SNP at rs1663689 represses ADGRG6 via interchromosomal interaction and reduces lung cancer progression. EMBO reports, 2023 (PubMed: 37154297) [IF=6.5]
4). Protective effects of 4-geranyloxy-2,6-dihydroxybenzophenonel on DSS-induced ulcerative colitis in mice via regulation of cAMP/PKA/CREB and NF-κB signaling pathways. Phytotherapy Research, 2023 (PubMed: 36428266) [IF=6.1]
5). Knockout of Stearoyl-CoA Desaturase 1 Decreased Milk Fat and Unsaturated Fatty Acid Contents of the Goat Model Generated by CRISPR/Cas9. Journal of agricultural and food chemistry, 2022 (PubMed: 35343224) [IF=5.7]
6). GnRH-driven FTO-mediated RNA m6A modification promotes gonadotropin synthesis and secretion. BMC biology, 2024 (PubMed: 38702712) [IF=5.4]

Application: WB    Species: Rat    Sample:

Fig. 3 FOXP2 regulates gonadotropin synthesis and secretion via activating the cAMP/PKA signaling pathway. A WB analysis cAMP, PKA, and p-PKA transfected with vector (Vector group) or FOXP2 overexpression plasmid (FOXP2 group) in LβT2 cells and primary rat adenohypophysis cells. B Detection of intracellular cAMP levels transfected with vector (Vector group) or FOXP2 overexpression plasmid (FOXP2 group) in LβT2 cells (left) and primary rat adenohypophysis cells (right). C Statistical analysis of cAMP protein expression changes in LβT2 cells (left, n = 2) and primary rat adenohypophysis cells (right, n = 2). D Statistical analysis of p-PKA protein expression changes in LβT2 cells (left, n = 2) and primary rat adenohypophysis cells (right, n = 2). E, F FOXP2 overexpression plasmid or vector was transfected into LβT2 cells with or without the indicated compounds (DDA, 10 μM; H89, 10 μM). The expression of Fshb and Lhb mRNA was assayed by RT-qPCR (E, n = 3). The secretion of FSH and LH was assayed by ELISA (F, n = 5). G, H FOXP2 siRNA or siNC were transfected into LβT2 cells with or without the indicated compounds (8-Bromo-cAMP, 500 μM; H89, 10 μM). The expression of Fshb and Lhb mRNA was assayed by RT-qPCR (G, n = 3). The secretion of FSH and LH was assayed by ELISA (H, n = 5). *, P 
7). Study of the mechanism by which Xiaoyan decoction combined with E7449 regulates tumorigenesis in lung adenocarcinoma. Journal of cellular and molecular medicine, 2024 (PubMed: 38898581) [IF=5.3]
8). Combination of pseudoephedrine and emodin ameliorates LPS-induced acute lung injury by regulating macrophage M1/M2 polarization through the VIP/cAMP/PKA pathway. Chinese Medicine, 2022 (PubMed: 35123524) [IF=5.3]

Application: WB    Species: Rat    Sample: lung tissues 

Fig. 6 Pseudoephedrine + emodin up-regulated VIP/CAMP/PKA pathways and Inhibited NF-κB in LPS-induced acute lung injury in rats. A, D VIP, cAMP mRNA expression was determined using Real-time PCR analysis. B, C, E–H Western blot analysis was performed to detect VIP, cAMP, p-PKA, p-IκBα and p-P65 protein expression. All data are expressed as mean ± S.D. (n = 3). ##p < 0.01, ###p < 0.001 vs. control group. *p < 0.05, **p < 0.01, ***p < 0.001 vs. LPS alone group. +p < 0.05, ++p < 0.01, +++p < 0.001 vs. combined treatment group (5 + 20 mg/kg)
9). NPY stimulates cholesterol synthesis acutely by activating the SREBP2-HMGCR pathway through the Y1 and Y5 receptors in murine hepatocytes. LIFE SCIENCES, 2020 (PubMed: 32976883) [IF=5.2]

Application: WB    Species: rat    Sample: BRL-3A hepatocytes

Fig. 6. | NPY activates the phosphorylation of ERK1/2 but not PKA.(B) NPY (25 nM) could not stimulate PKA phosphorylation at Thr197/Thr198 site in BRL-3A hepatocytes. The results are presented as the percentage change in phospho-ERK/ERK or phospho-PKA/PKA intensity compared with the control and normalized to 100%. The data are presented as the mean ± SE; *P < 0.05, **P < 0.01; one-way analysis of variance (ANOVA) followed by post hoc Dunnett's test in (A and B).
10). Inhibition of reinstatement of alcohol-induced conditioned place preference in mice by Lonicera japonica polysaccharide. Food & Function, 2022 (PubMed: 35899807) [IF=5.1]
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