产品: Osteopontin 抗体
货号: AF0227
描述: Rabbit polyclonal antibody to Osteopontin
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Dog
分子量: 60kDa; 35kD(Calculated).
蛋白号: P10451
RRID: AB_2833402

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(88%), Dog(80%)
克隆:
Polyclonal
特异性:
Osteopontin Antibody detects endogenous levels of total Osteopontin.
RRID:
AB_2833402
引用格式: Affinity Biosciences Cat# AF0227, RRID:AB_2833402.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BNSP; Bone sialoprotein 1; BSP I; BSPI; Early T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; OPN; Osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; OSTP_HUMAN; PSEC0156; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1; SPP 1; SPP-1; SPP1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P10451 OSTP_HUMAN:

Bone. Found in plasma.

描述:
osteopontin Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. Ligand for integrin alpha-V/beta-3. 4 isoforms of the human protein are produced by alternative splicing.
序列:
MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Bovine
88
Dog
80
Sheep
78
Pig
73
Horse
67
Rabbit
33
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P10451 作为底物

Site PTM Type Enzyme
S24 Phosphorylation
S26 Phosphorylation
S27 Phosphorylation
S49 O-Glycosylation
S49 Phosphorylation
S62 Phosphorylation
S63 Phosphorylation
T66 Phosphorylation
S76 Phosphorylation
S78 Phosphorylation
S81 Phosphorylation
S99 Phosphorylation
S102 Phosphorylation
S105 Phosphorylation
S108 Phosphorylation
S117 Phosphorylation
S120 Phosphorylation
S123 Phosphorylation
S126 Phosphorylation
S129 Phosphorylation
T134 O-Glycosylation
T134 Phosphorylation
T138 O-Glycosylation
T138 Phosphorylation
T143 O-Glycosylation
T143 Phosphorylation
T147 O-Glycosylation
T147 Phosphorylation
T152 O-Glycosylation
T152 Phosphorylation
Y165 Phosphorylation
T185 Phosphorylation
T190 Phosphorylation
S191 Phosphorylation
S195 Phosphorylation
Y202 Phosphorylation
S215 Phosphorylation
S219 Phosphorylation
S224 Phosphorylation
Y225 Phosphorylation
T227 Phosphorylation
S228 Phosphorylation
S234 Phosphorylation
T237 Phosphorylation
S239 Phosphorylation
S243 Phosphorylation
S254 Phosphorylation
S258 Phosphorylation
S263 Phosphorylation
S267 Phosphorylation
S270 Phosphorylation
S275 Phosphorylation
S280 Phosphorylation
S291 Phosphorylation
S303 Phosphorylation
S308 Phosphorylation
S310 Phosphorylation
S311 Phosphorylation

研究背景

功能:

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

翻译修饰:

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.

O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Bone. Found in plasma.

亚基结构:

Ligand for integrin alpha-V/beta-3.

蛋白家族:

Belongs to the osteopontin family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

文献引用

1). Zeng J et al. Osteoblastic and anti-osteoclastic activities of strontium-substituted silicocarnotite ceramics: In vitro and in vivo studies. Bioactive Materials 2020 Apr 6;5(3):435-446. (PubMed: 32280833) [IF=18.9]

2). Chen J et al. Fusion peptide engineered “statically-versatile” titanium implant simultaneously enhancing anti-infection, vascularization and osseointegration. Biomaterials 2021 Jan;264:120446 (PubMed: 33069134) [IF=14.0]

3). Chen J et al. On-demand storage and release of antimicrobial peptides using Pandora's box-like nanotubes gated with a bacterial infection-responsive polymer. Theranostics 2020 Jan 1;10(1):109-122 (PubMed: 31903109) [IF=12.4]

Application: WB    Species: Human    Sample: Human bone mesenchymal stem cells(hBMSCs)

Figure 5. In vitro biocompatibilities and osteogenic activities of the substrates. (A) CCK-8 assay of hBMSCs on the indicated surfaces after 1, 3 and 7 days of culture. * denotes p < 0.05 and ** denotes p < 0.01 compared to Ti-NTs, # denotes p < 0.05 compared to the corresponding control group without peptides. Error bars denote the standard deviations over quadruplicate measurements with separately implants. (B) Confocal fluorescence microscopy images of hBMSCs stained with vinculin, F-actin and DAPI after being cultured for 24 h. Scale bar, 50 μm. (C-F) qRT-PCR assay of osteogenic gene expression of (C) ALP, (D) RUNX-2, (E) COL1 and (F) OPN of hBMSCs after 7 and 14 days of culture. * denotes p < 0.05, ** denotes p < 0.01 and *** denotes p < 0.001 compared to Ti-NTs-P-A; # denotes p < 0.05, & denotes p < 0.01 and $ denotes p < 0.001 compared to the corresponding control group without peptides. All error bars denote the standard deviations over quadruplicate measurements with separately implants. (G) Immunofluorescence staining of hBMSCs cultured on Ti-NTs-P-A for 7 days (ALP and RUNX-2) and 14 days (OPN). The images were obtained by confocal fluorescence microscopy. Scale bar, 50 μm. (H) Western blotting of hBMSCs cultured on the substrates for 7 and 14 days. At each time point, left lane was Ti-NTs, middle lane was Ti-NTs-A and right lane was Ti-NTs-P-A. * denotes p < 0.05, ** denotes p < 0.01 and *** denotes p < 0.001 compared to Ti-NTs-P-A. Error bars denote the standard deviations over triplicate measurements with separately Western blotting results.

4). Biomimetic hydroxyapatite coating on the 3D-printed bioactive porous composite ceramic scaffolds promoted osteogenic differentiation via PI3K/AKT/mTOR signaling pathways and facilitated bone regeneration in vivo. Journal of Materials Science & Technology [IF=10.9]

5). Wang et al. A scaffold with zinc-whitlockite nanoparticles accelerates bone reconstruction by promoting bone differentiation and angiogenesis. Nano Research [IF=9.9]

6). Yuan P et al. Kaempferol alleviates calcium oxalate crystal-induced renal injury and crystal deposition via regulation of the AR/NOX2 signaling pathway. PHYTOMEDICINE 2021 Mar 27;86:153555. (PubMed: 33852977) [IF=7.9]

Application: IHC    Species: Human    Sample: HK-2 cells

Fig. 1. Effect of kaempferol on renal CaOx crystal deposition and tubular injury in vivo. Eligible mice were divided into five groups (n = 8). After a period of 10-day drug treatments, all mice were sacrificed on the 11 th day for the following tests. Pathological results were shown by the (a) images as well as (b-g) corresponding quantitative analysis through HE staining, Pizzolato staining, PAS staining, TUNEL staining, OPN and CD44 immunohistochemistry staining of paraffin embedded kidney sections. (h-j) Renal dysfunction was determined by serum levels of BUN, creatinine and NAGL among the five groups. (k) Group annotations were applied for all histograms. Data were expressed as the fold changes of experimental group to NC group or GA group, and were represented as means ± SD. * p < 0.05 vs. NC group; ** p < 0.05 as GA+Kae (25mg/kg) vs. GA group, GA + Kae (50 mg/kg) vs. GA group, and GA + Kae (25 mg/kg) vs. GA + Kae (50 mg/kg) group, respectively.

7). Zhou J et al. Strontium-Containing Barium Titanate-Modified Titanium for Enhancement of Osteointegration. ACS Biomaterials Science & Engineering 2022 Feb 10. (PubMed: 35143178) [IF=5.8]

8). Zhou M et al. Synergistically Promoting Bone Regeneration by Icariin-Incorporated Porous Microcarriers and Decellularized Extracellular Matrix Derived From Bone Marrow Mesenchymal Stem Cells. Frontiers in Bioengineering and Biotechnology 2022 Apr 7;10:824025. (PubMed: 35464719) [IF=5.7]

Application: IF/ICC    Species: Rat    Sample: BMSCs

FIGURE 8 The expression of osteogenesis-related genes and proteins: (A) Immunofluorescent images of Col-I and OPN expressed by BMSCs cultured on the different microcarriers for 14 days which were observed under CLSM (Scale bar = 50 μm); (B,C) qRT-PCR analysis of Col-I and OPN. (*p < 0.05, **p < 0.01, n = 3).

9). Liu et al. Hypoxia‑induced mitophagy regulates proliferation, migration and odontoblastic differentiation of human dental pulp cells through FUN14 domain‑containing 1. International Journal of Molecular Medicine 2022 May;49(5):72. (PubMed: 35362539) [IF=5.4]

Application: WB    Species: Human    Sample: HDPCs

Figure 3 Hypoxia promotes proliferation, migration and odontoblastic differentiation in HDPCs. (A) Representative images of wound healing assays in the normoxic or hypoxic group. Scale bar, 1 mm. (B) Percentages of wound closure from three independent experiments are quantified. (C) Representative images of migratory cells stained with crystal violet. (D) Statistical quantification of the number of migratory cells from three independent experiments are shown. Scale bar, 100 µm. (E) Cell viability of HDPCs was detected using Cell Counting Kit-8 analysis. (F) ALP staining and (G) activity in the normoxic or hypoxic group on day 3. Scale bar, 200 µm. (H) Representative western blotting images showing the protein expression levels of RUNX2, Col I, OSX and OPN in the normoxic or hypoxic group on day 3, (I) which were quantified. Results are presented as the means ± SD from ≥ three independent experiments. **P<0.01 and ***P<0.001 vs. normoxia. Nor, normoxia; Hypo, hypoxia; OD, optical density; ALP, alkaline phosphatase; RUNX2, runt-related transcription factor 2; Col I, collagen type I; OSX, osterix; OPN, osteopontin.

10). Lu H et al. C/EBPα-Mediated Transcriptional Activation of PIK3C2A Regulates Autophagy, Matrix Metalloproteinase Expression, and Phenotypic of Vascular Smooth Muscle Cells in Aortic Dissection. Journal of Immunology Research 2022 Sep 12;2022:7465353. (PubMed: 36132983) [IF=4.1]

Application: WB    Species: Rat    Sample: VSMCs

Figure 4 The impact of C/EBPα in angiotensin II- (Ang II-) treated VSMCs. The relative protein expressions in Ang II-treated VSMCs with presence of C/EBPα overexpression or knockdown were analyzed by Western blot. GAPDH was used as an internal control. Data are presented as means ± SEM. (a, b) Evaluation of the influence of C/EBPα on the expression of α-SMA, SM-MHC, and OPN proteins. (c, d) Evaluation of the influence of C/EBPα on the expression of PIK3C2A protein. (e, f) Evaluation of the influence of C/EBPα on the expression of Beclin-1, p62, LC3II, and LC3I proteins. (g, h) Evaluation of the influence of C/EBPα on the expression of MMP-2 and MMP-9 proteins. (i) Intracellular ROS was measured by flow cytometry using DCFH-DA probe with presence of C/EBPα overexpression or knockdown. Control: control group; model: treated with 1 μM Ang II; vector: treated with 1 μM Ang II in vector transfected VSMCs; pcC/EBPα: treated with 1 μM Ang II in C/EBPα-overexpressed VSMCs; siNC: treated with 1 μM Ang II in siRNA NC-transfected VSMCs; siC/EBPα: treated with 1 μM Ang II in C/EBPα-knockdown VSMCs. ∗P < 0.05 vs. control,

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