Phospho-RPS6 (Tyr209) Antibody - #AF7331

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产品: 磷酸化 RPS6 (Tyr209) 抗体
货号: AF7331
描述: Rabbit polyclonal antibody to Phospho-RPS6 (Tyr209)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 32kDa; 29kD(Calculated).
蛋白号: P62753
RRID: AB_2843771

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(90%)
克隆:
Polyclonal
特异性:
Phospho-RPS6 (Tyr209) Antibody detects endogenous levels of RPS6 only when phosphorylated at Tyr209.
RRID:
AB_2843771
引用格式: Affinity Biosciences Cat# AF7331, RRID:AB_2843771.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

40S ribosomal protein S6; Air8; NP33; Phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; rps6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein;

抗原和靶标

免疫原:
Uniprot:

P62753(RS6_HUMAN) >>Visit HPA database.

基因/基因ID:
RPS6(6194)
序列:
MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
90
Chicken
90
Zebrafish
89
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P62753 作为底物

Site PTM Type Enzyme
K2 Methylation
S6 Phosphorylation
C12 S-Nitrosylation
K14 Acetylation
K14 Ubiquitination
R22 Methylation
K23 Ubiquitination
Y28 Phosphorylation
K30 Acetylation
K30 Ubiquitination
K46 Ubiquitination
S53 Phosphorylation
K58 Ubiquitination
K64 Ubiquitination
T69 Phosphorylation
S78 Phosphorylation
K79 Acetylation
K79 Ubiquitination
S82 Phosphorylation
K116 Ubiquitination
K119 Ubiquitination
T128 Phosphorylation
K143 Ubiquitination
S148 Phosphorylation
K149 Ubiquitination
R154 Methylation
K160 Ubiquitination
T181 Phosphorylation
K203 Ubiquitination
Y209 Phosphorylation
K211 Acetylation
K211 Ubiquitination
K218 Acetylation
S235 Phosphorylation Q13177 (PAK2) , P51812 (RPS6KA3) , P53355 (DAPK1) , Q05655 (PRKCD) , P23443 (RPS6KB1) , Q96RG2 (PASK) , Q15418 (RPS6KA1)
S236 Phosphorylation Q05655 (PRKCD) , P23443 (RPS6KB1) , P51812 (RPS6KA3) , Q13177 (PAK2) , Q15418 (RPS6KA1) , Q96RG2 (PASK)
S240 Phosphorylation Q13177 (PAK2) , P23443 (RPS6KB1) , Q15418 (RPS6KA1)
T241 Phosphorylation
S242 Phosphorylation Q13177 (PAK2)
S244 Phosphorylation Q15418 (RPS6KA1) , P23443 (RPS6KB1)
S246 Phosphorylation
S247 Phosphorylation P23443 (RPS6KB1)

研究背景

功能:

May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

翻译修饰:

Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Specifically hydroxylated (with R stereochemistry) at C-3 of Arg-137 by KDM8.

蛋白家族:

Belongs to the eukaryotic ribosomal protein eS6 family.

研究领域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > Ribosome.

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

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