产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3205, RRID:AB_2834637.
展开/折叠
PTP1B; Non receptor tyrosine phosphatase 1; Protein phosphotyrosylphosphatase 1B; Protein tyrosine phosphatase 1B; Protein tyrosine phosphatase non receptor type 1; Protein tyrosine phosphatase placental; Protein-tyrosine phosphatase 1B; PTN1_HUMAN; PTP 1B; PTP-1B; PTPN 1; PTPN1; Tyrosine protein phosphatase non receptor type 1; Tyrosine-protein phosphatase non-receptor type 1;
抗原和靶标
- P18031 PTN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEMEKEFEQIDKSGSWAAIYQDIRHEASDFPCRVAKLPKNKNRNRYRDVSPFDHSRIKLHQEDNDYINASLIKMEEAQRSYILTQGPLPNTCGHFWEMVWEQKSRGVVMLNRVMEKGSLKCAQYWPQKEEKEMIFEDTNLKLTLISEDIKSYYTVRQLELENLTTQETREILHFHYTTWPDFGVPESPASFLNFLFKVRESGSLSPEHGPVVVHCSAGIGRSGTFCLADTCLLLMDKRKDPSSVDIKKVLLEMRKFRMGLIQTADQLRFSYLAVIEGAKFIMGDSSVQDQWKELSHEDLEPPPEHIPPPPRPPKRILEPHNGKCREFFPNHQWVKEETQEDKDCPIKEEKGSPLNAAPYGIESMSQDTEVRSRVVGGSLRGAQAASPAKGEPSLPEKDEDHALSYWKPFLVNMCVATVLTAGAYLCYRFLFNSNT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P18031 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K5 | Ubiquitination | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| Y20 | Phosphorylation | Uniprot | |
| C32 | S-Nitrosylation | Uniprot | |
| Y46 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | P49759 (CLK1) , P31749 (AKT1) , P49760 (CLK2) | Uniprot |
| S55 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| Y66 | Phosphorylation | P00533 (EGFR) , P06213 (INSR) | Uniprot |
| S70 | Phosphorylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| C92 | S-Nitrosylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K131 | Acetylation | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| K141 | Acetylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| Y152 | Phosphorylation | P06213 (INSR) | Uniprot |
| Y153 | Phosphorylation | P06213 (INSR) | Uniprot |
| S201 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| C215 | S-Nitrosylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S242 | Phosphorylation | P49759 (CLK1) , P49760 (CLK2) | Uniprot |
| S243 | Phosphorylation | P49759 (CLK1) , P49760 (CLK2) | Uniprot |
| K247 | Ubiquitination | Uniprot | |
| S286 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S295 | Phosphorylation | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| K335 | Sumoylation | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| T338 | Phosphorylation | Uniprot | |
| K347 | Sumoylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| S352 | Phosphorylation | P68400 (CSNK2A1) , P19784 (CSNK2A2) | Uniprot |
| Y359 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| T368 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | P68400 (CSNK2A1) , P17252 (PRKCA) , P19784 (CSNK2A2) | Uniprot |
| R380 | Methylation | Uniprot | |
| S386 | Phosphorylation | P06493 (CDK1) , P68400 (CSNK2A1) , P19784 (CSNK2A2) | Uniprot |
| S393 | Phosphorylation | P53350 (PLK1) | Uniprot |
研究背景
Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.
S-nitrosylation of Cys-215 inactivates the enzyme activity.
Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.
Endoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Interacts with EPHA3 at the cell membrane.
Expressed in keratinocytes (at protein level).
Interacts with EPHA3 (phosphorylated); dephosphorylates EPHA3 and may regulate its trafficking and function. Interacts with MET.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.