Cytokeratin 7 Antibody - #AF0195

Figure 2. - Effect of KRT7 on HEY and OVCAR433 cell proliferation and colony formation. (A) Protein expression levels of KRT7 in ovarian cell lines were assessed using western blotting. (B) Overexpression of KRT7 in HEY cells was confirmed by western blotting. (C) Overexpression of KRT7 significantly increased the proliferative capacity of HEY cells. (D) Overexpression of KRT7 in HEY cells significantly increased colony formation. (E) Knockdown of KRT7 in OVCAR433 cells was confirmed by western blotting. Knockdown of KRT7 in OVCAR433 cells significantly reduced (F) cell proliferation and (G) colony formation. All experiments were performed at least three times. Results are presented as the mean ± standard deviation. **P<0.01. KRT7, keratin 7; sh, short hairpin RNA; NC, negative control; OD, optical density.

Figure 1. - KRT7 expression is indicative of a poor prognosis in patients with ovarian cancer. (A) Representative images of KRT7 staining in normal and ovarian tumor tissues, and immunohistochemical score of KRT7 in normal and ovarian tumor tissues. (B) mRNA expression levels of KRT7 in normal and ovarian tumor tissues. Associations between the (C) pathological grades and (D) clinical stages of ovarian cancer with KRT7 expression. (E) Effect of mRNA expression levels of KRT7 on the median survival time of patients with ovarian cancer. (F) Gene Ontology analysis of the differentially expressed genes between the high- and low-expression KRT7 groups in patients with ovarian cancer. Data were obtained from the Human Protein Atlas and The Cancer Genome Atlas database. *P<0.05, **P<0.01. KRT7, keratin 7; ECM, extracellular matrix.

Fig. 2. Identification of human sweat gland cells and verification of integrin β6 (ITGB6) overexpression transfection efficiency. A. Immunofluorescence staining of CEA (green) and CK7 (red) in sweat gland cells extracted from patients with primary palmar hyperhidrosis. The cell nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (blue); B. Quantitative polymerase chain reaction (ITGB6 compared to control, Tukey’s honest significant difference (HSD), p < 0.001; ITGB6 compared to negative control (NC), Tukey’s HSD, p < 0.001); C,D. Western blot detection of the transfection efficiency of ITGB6 overexpression analysis results (ITGB6 compared to control, Tukey’s HSD, p < 0.001; ITGB6 compared to NC, Tukey’s HSD, p < 0.001). The data are presented as mean ± 95% confidence interval (95% CI)

FIGURE 4 Immunostaining of cytoskeletal proteins of the ceruminous gland of a (a) human, (b) goat, (c) dog, and (d) pig. CK7, CK8, CK18, and CK19 are expressed in the EAC glandular cells of humans, goats, dogs, and pig. The positive expressions appeared to be brown or dark brown. Scale bar = 50 μm