Phospho-MITF (Ser180) Antibody - #AF3027

Figure 5. The comparison of core melanogenesis genes in skins between WT and YM snakeheads. (a) Western blotting detection of TYR protein levels in skins of WT and YM snakeheads (mean ± s.e.m., n = 4). (b) qPCR detection of mRNA levels of the melanogenic genes (tyr, tyrp1a, tyrp1b, dct and pmel) at different ontogenetic stages (mean ± s.e.m., n = 3). (c) qPCR detection of mRNA levels of mitfa and mitfb in skins of WT and YM snakeheads (mean ± s.e.m., n = 3). (d) Western blotting detection of phospho-MITF(Ser180) protein levels in skins of WT and YM snakeheads (mean ± s.e.m., n = 4). The western blots are cropped and blot source data are available in the electronic supplementary material, figures S3 and S4. * p < 0.05, ** p < 0.01, *** p < 0.001, n.s.: not significant.

FIGURE 2|CDP promotes melanogenesis in HEMs. The HEMs were treated with CDP at different concentrations (20, 40 and 80 μg/mL) or medium alone (NC) for 48 h, then we observed the melanin by Fontana-Masson staining, measured the melanin content (OD value, 470 nm) by NaOH assay, and measured the tyrosinase activity (OD value, 475 nm) by tyrosinase activity assay; meanwhile, we measured the protein levels of MITF, p-MITF, TYR, TRP1, TRP2, RAB27A and FSCN1 by Western blotting and measure the grey value of protein bands by Image J: (A) melanin staining; (B) the melanin content; (C) the tyrosinase activity; (D) the protein levels of melanogenesis-related genes

Fig. 8.| (A) Phosphorylated MpMITF (p-MITF) expression in the haemocytes of M. petechialis in the control group and p38 inhibitor group, as measured by Western blot. β-actin was used as the internal reference to normalize the total protein quantity between samples.

Fig. 6 LDF promote melanogenesis in juvenile zebrafish. A. Toxicity assays in embryos with drugs administration of LDF (0–120 μg/ml); B. The effect of LDF on melanogenesis with drugs administration of LDF (20–80 μg/ml and 1 μM methoxsalen) for 72 h; C-D. The effect of LDH on melanin contents; E. The effect of LDH on the protein expression of MITF, p-MITF, TYR, TRP1, and TRP2.