Phospho-GSK3 beta (Ser9) Antibody - #AF2016

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产品: 磷酸化 GSK3 beta (Ser9) 抗体
货号: AF2016
描述: Rabbit polyclonal antibody to Phospho-GSK3 beta (Ser9)
应用: WB IHC IF/ICC IP
文献验证: WB, IHC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Zebrafish, Rabbit, Dog, Xenopus
蛋白号: P49841
RRID: AB_2834439

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:500, IF/ICC 1:100-1:500, IP 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
Phospho-GSK3 beta (Ser9) Antibody detects endogenous levels of GSK3 beta only when phosphorylated at Serine 9.
RRID:
AB_2834439
引用格式: Affinity Biosciences Cat# AF2016, RRID:AB_2834439.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Glycogen Synthase Kinase 3 Beta; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK-3 beta; GSK3B; GSK3B_HUMAN; GSK3beta isoform; Serine/threonine-protein kinase GSK3B;

抗原和靶标

免疫原:

A synthesized peptide derived from human GSK3 beta around the phosphorylation site of Ser9.

基因/基因ID:
GSK3B(2932)
描述:
GSK3B a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation .

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

文献引用

1). MicroRNA-200c impairs uterine receptivity formation by targeting FUT4 and α1,3-fucosylation. CELL DEATH AND DIFFERENTIATION, 2017 (PubMed: 28914881) [IF=13.7]

Application: WB    Species: human    Sample:

Figure 4 miR-200c decreases α1,3-fucosylation on CD44 and inactivates Wnt/β-catenin signaling pathway. (a, e) Western/lectin blot analysis of effect of miR-200c on α1,3- fucosylation and LeY biosynthesis in RL95-2 (a) and Ishikawa (e) cells. CBB: coomassie brilliant blue. LTL: Lotus tetragonolobus lectin. (b, f) Immunoprecipitation and western blot analysis of α 1,3-fucosylation and LeY on CD44 in RL95-2 (b) and Ishikawa (f) cells. Immunoprecipitation (IP): anti-CD44 antibody pulled down protein. Immune blot (IB): detection of α 1,3-fucosylation by LTL lectin and anti-LeY antibody. (c, g) Western blot analysis of CD44, LTL and LeY blocking on activation of p-GSK3β, GSK3β and β-catenin in RL95-2 (c) and Ishikawa (g) cells. (d, h) Western blot and statistical analysis of p-GSK3β, GSK3β and β-catenin in RL95-2 (d) and Ishikawa (h) cells. DKK: inhibitor of Wnt/β- catenin signal pathway. *Po0.05, **Po0.01, ***Po0.
2). Polydatin accelerates osteoporotic bone repair by inducing the osteogenesis-angiogenesis coupling of bone marrow mesenchymal stem cells via the PI3K/AKT/GSK-3β/β-catenin pathway. International journal of surgery (London, England), 2025 (PubMed: 39248296) [IF=12.5]
3). Polydatin accelerates osteoporotic bone repair by inducing the osteogenesis-angiogenesis coupling of bone marrow mesenchymal stem cells via the PI3K/AKT/GSK-3β/β-catenin pathway. International journal of surgery (London, England), 2024 (PubMed: 39248296) [IF=12.5]

Application: WB    Species: Rat    Sample:

Figure 3. The effect of POL action was via the PI3K/AKT/GSK3-β/β-catenin pathway. (A-B) The protein expression levels of total PI3K, p-PI3K, total AKT, pAKT, total GSK3β, p-GSK3β, total β-catenin, and p-β-catenin were evaluated by western blot (n = 3); (C) Images of immunofluorescence staining for β-catenin (scale bar = 100 μm); (D-E) Quantification of immunofluorescence staining for β-catenin (n = 5). Data were presented as mean ± SEM. Compared with control group: * P < 0.05, **P < 0.01, ***P < 0.001. Compared with POL group: ##P < 0.01, ### P < 0.001.
4). Baicalin ameliorates neuroinflammation-induced depressive-like behavior through inhibition of toll-like receptor 4 expression via the PI3K/AKT/FoxO1 pathway. Journal of Neuroinflammation, 2019 (PubMed: 31068207) [IF=9.3]

Application: WB    Species: mouse    Sample: hippocampus

Fig. 10 | A discussion of the potential targets of baicalin. c Western blot was performed to measure the levels of p-AKT and p-GSK3β in the hippocampus of CUMS mice after treatment with LY294002 and baicalin (n = 4).Data are presented as means ± SEM. *p < 0.05, **p < 0.01 vs. control; #p < 0.05, ##p < 0.01 vs. CUMS; &p < 0.05, &&p < 0.01 vs. CUMS+BA60
5). m6A methylation-mediated PGC-1α contributes to ferroptosis via regulating GSTK1 in arsenic-induced hepatic insulin resistance. The Science of the total environment, 2023 (PubMed: 37730054) [IF=8.2]

Application: WB    Species: human    Sample: L-02. Cells

Fig. 1. Ferroptosis was involved in sodium arsenite (NaAsO2)-induced hepatic insulin resistance (IR). (A–C) Relative acyl-CoA synthetase long-chain family member 4 (ACLS4) and glutathione peroxidase 4 (GPX4) expression in rat livers. (D–H) The relative content of liver iron, serum iron, Glutathione (GSH), total-superoxide dismutase (T-SOD), and malondialdehyde (MDA) in the control and NaAsO2-treated rats. (I–K) Expression of total and phosphorylated AKT at Ser 473 (p-AKT) and glycogen synthase kinase 3 beta (p-GSK3β) at Ser 9 in L-02. Cells were pretreated with ferrostatin-1 (Fer-1) for 4 h before treatment with 4 μM NaAsO2 for 48 h. Insulin was applied for 10 min before the cells were collected. (L) The effect of Fer-1 on insulin-stimulated glucose uptake in cells. **P < 0.01 versus the control; #P < 0.05 and ##P < 0.01 versus the NaAsO2 (n = 3).
6). Oxyberberine, an absorbed metabolite of berberine, possess superior hypoglycemic effect via regulating the PI3K/Akt and Nrf2 signaling pathways. BIOMEDICINE & PHARMACOTHERAPY, 2021 (PubMed: 33524788) [IF=6.9]
7). Ursolic Acid promotes apoptosis and mediates transcriptional suppression of CT45A2 gene expression in NSCLCs harboring EGFR T790M. British Journal of Pharmacology, 2019 (PubMed: 31322286) [IF=6.8]

Application: WB    Species: human    Sample: H1975 cells

FIGURE 6| Ursolic acid (UA) inhibited β-catenin/TCF4 signalling pathway in H1975 cells. (D) Left: Protein expression of β-catenin, GSK-3β and their phosphorylated forms, were determined by western blot analysis. Phosphorylation of β-catenin and GSK-3β was decreased after UA treatment. Erlotinib was used as the negative control. Right: The graph shows the mean values from western blotting. Protein levels were quantified using gray value analyses by Image J software.
8). A possible mechanism to the antidepressant-like effects of 20 (S)-protopanaxadiol based on its target protein 14-3-3 ζ. Journal of Ginseng Research, 2022 (PubMed: 36090685) [IF=6.8]

Application: WB    Species: Rat    Sample:

Fig. 5 Effects of fluoxetine (FLU) or PPD on the immobility time in the forced swimming test (FST) (A) and tail suspension test (TST) (B) in the corticosterone (CORT)-induced depression model. Representative Western blot images of GSK 3β, p-Ser9 GSK 3β, CREB, p-Ser133 CREB, BDNF, and β-actin expression levels (C). Western blot analysis showing relative protein levels of p-Ser133 CREB (D), p-Ser9 GSK 3β (E), and BDNF (F). ∗p < 0.05 vs. the control group, nsno statistical significance vs. the control group, #p < 0.05 vs. the CORT group.
9). Quercetin induces MGMT+ glioblastoma cells apoptosis via dual inhibition of Wnt3a/β-Catenin and Akt/NF-κB signaling pathways. Phytomedicine, 2023 (PubMed: 37451151) [IF=6.7]
10). 2-Ethylhexyl diphenyl phosphate aggravates colitis-induced neuroinflammation and behavioral abnormalities by inhibiting the PI3K-AKT-NF-κB and Wnt/GSK3β signaling pathways. Ecotoxicology and environmental safety, 2024 (PubMed: 39405964) [IF=6.2]

Application: WB    Species: Mouse    Sample:

Fig. 4. EHDPHP aggravated neuroinflammation and neurobehavioral impairments by inhibiting the Wnt/GSK3β signaling pathway. A, B: Western blots showing the protein expression levels of GSK3β, P-GSK3β and β-catenin. Significant differences were evaluated with one-way ANOVA followed by the Tukey post hoc test for multiple comparisons. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).
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