Phospho-FOXO3A (Ser253) Antibody - #AF3020

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产品: 磷酸化 FOXO3A (Ser253) 抗体
货号: AF3020
描述: Rabbit polyclonal antibody to Phospho-FOXO3A (Ser253)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: O43524
RRID: AB_2834427

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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-FOXO3A (Ser253) Antibody detects endogenous levels of FOXO3A only when phosphorylated at Serine 253.
RRID:
AB_2834427
引用格式: Affinity Biosciences Cat# AF3020, RRID:AB_2834427.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AF6q21; AF6q21 protein; DKFZp781A0677; FKHR2; FKHRL 1; FKHRL1; FKHRL1P2; Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1; Forkhead box O3; Forkhead box O3A; Forkhead box protein O3; Forkhead box protein O3A; Forkhead Drosophila homolog of in rhabdomyosarcoma like 1; Forkhead homolog (rhabdomyosarcoma) like 1; Forkhead in rhabdomyosarcoma like 1; Forkhead in rhabdomyosarcoma-like 1; FOX O3A; FOXO2; foxo3; FOXO3_HUMAN; FOXO3A; MGC12739; MGC31925;

抗原和靶标

免疫原:

A synthesized peptide derived from human FOXO3A around the phosphorylation site of Ser253.

基因/基因ID:
FOXO3(2309)
描述:
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death.

研究领域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

文献引用

1). Exercise-induced Musclin determines the fate of fibro-adipogenic progenitors to control muscle homeostasis. Cell stem cell, 2024 (PubMed: 38232727) [IF=19.8]
2). Thread-structural microneedles loaded with engineered exosomes for annulus fibrosus repair by regulating mitophagy recovery and extracellular matrix homeostasis. Bioactive materials, 2024 (PubMed: 38515611) [IF=18.9]
3). Sleep Deprivation Triggers the Excessive Activation of Ovarian Primordial Follicles via β2 Adrenergic Receptor Signaling. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 39229959) [IF=15.1]
4). Bexarotene improves motor function after spinal cord injury in mice. Neural Regeneration Research, 2023 (PubMed: 37449638) [IF=5.9]

Application: WB    Species: Mouse    Sample: spinal cord

Figure 10 Bexarotene activates TFE3 through the AMPK-mTOR and AMPK-SPK2- CARM1 signaling pathways in SCI. (A) Western blot assay of the cytoplasmic expression levels of AMPK, p-AMPK, mTOR, and p-mTOR. (B) Quantification of AMPK, p-AMPK, mTOR, and p-mTOR from A, normalized to GADPH. (C) Western blot assay of the nuclear expression levels of AMPK, p-AMPK, FOXO3a, p-FOXO3a, SKP2, and CARM1. (D) Quantification of AMPK, p-AMPK, FOXO3a, p-FOXO3a, SKP2, and CARM1 from C, normalized to H3. (E, F) Immunoprecipitation images showing nuclear CARM1–TFE3 binding. Data are expressed as the mean ± SEM (n = 6 per group). **P < 0.01, vs. SCI group; ##P < 0.01, vs. SCI + Bex group. One-way analysis of variance with least significance difference post hoc test. (p-)AMPK: (phospho-) adenosine 5′-monophosphate-activated protein kinase; (p-)FOXO3a: (phospho-)forkhead box O3; (p-)mTOR: (phospho-)mammalian target of rapamycin; Bex: bexarotene; CARM1: coactivator-associated arginine methyltransferase 1; CC: compound C; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; H3: histone protein H3; SCI: spinal cord injury; SKP2: S-phase kinase-associated protein 2; TFE3: transcription factor E3.
5). 贝沙罗汀改善脊髓损伤后运动功能的机制. 中国神经再生研究(英文版), 2023 (PubMed: 37449638) [IF=5.9]

Application: WB    Species: Mouse    Sample: spinal cord

Figure 10 Bexarotene activates TFE3 through the AMPK-mTOR and AMPK-SPK2- CARM1 signaling pathways in SCI. (A) Western blot assay of the cytoplasmic expression levels of AMPK, p-AMPK, mTOR, and p-mTOR. (B) Quantification of AMPK, p-AMPK, mTOR, and p-mTOR from A, normalized to GADPH. (C) Western blot assay of the nuclear expression levels of AMPK, p-AMPK, FOXO3a, p-FOXO3a, SKP2, and CARM1. (D) Quantification of AMPK, p-AMPK, FOXO3a, p-FOXO3a, SKP2, and CARM1 from C, normalized to H3. (E, F) Immunoprecipitation images showing nuclear CARM1–TFE3 binding. Data are expressed as the mean ± SEM (n = 6 per group). **P < 0.01, vs. SCI group; ##P < 0.01, vs. SCI + Bex group. One-way analysis of variance with least significance difference post hoc test. (p-)AMPK: (phospho-) adenosine 5′-monophosphate-activated protein kinase; (p-)FOXO3a: (phospho-)forkhead box O3; (p-)mTOR: (phospho-)mammalian target of rapamycin; Bex: bexarotene; CARM1: coactivator-associated arginine methyltransferase 1; CC: compound C; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; H3: histone protein H3; SCI: spinal cord injury; SKP2: S-phase kinase-associated protein 2; TFE3: transcription factor E3.
6). Luteolin inhibits triple-negative breast cancer by inducing apoptosis and autophagy through SGK1-FOXO3a-BNIP3 signaling. Frontiers in pharmacology, 2023 (PubMed: 37346292) [IF=5.6]

Application: WB    Species: Mouse    Sample: breast cancer cells

FIGURE 5. Luteolin encouraged apoptosis and autophagy in triple-negative breast cancer cells via the SGK1-FOXO3a-BNIP3 signaling pathway. (A) Differentially expressed genes involved in the FoxO signaling pathway. Red color represents upregulated genes and blue color represents downregulated genes. (B) Co-expression analysis of SGK1, FOXO3a, BNIP3, and apoptosis and autophagy-related genes by the string. (C) Expression levels of related protein of SGK1-FOXO3a-BNIP3 signaling pathways. (D) Expression levels of the apoptosis-related protein. (E) Expression levels of the autophagy-related protein. (F) The submicroscopic structural morphology was performed by transmission electron microscopy. Scale bar, 5 μm, and 1 μm. All results are represented as the mean ± SEM for at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ApoBDs: apoptotic bodies; AS: autophagosomes; ASS: autophagolysosomes.
7). Calycosin inhibited autophagy and oxidative stress in chronic kidney disease skeletal muscle atrophy by regulating AMPK/SKP2/CARM1 signalling pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020 (PubMed: 32910538) [IF=5.3]

Application: WB    Species: mouse    Sample: C2C12 cells

FIGURE 8| Effect of calycosin on the expression of proteins associated with the AMPK/SKP2/CARM1 signalling pathway in TNF-αinduced C2C12 cells in vitro. A, Representative images of H3R17me2a in C2C12 cells treated with TNF-α or calycosin. B-C, Representative immunoblot of key proteins associated with the AMPK/SKP2/CARM1 signalling pathway in C2C12 cells treated with TNF-α or calycosin.
8). Formononetin ameliorates muscle atrophy by regulating myostatin‐mediated PI3K/Akt/FoxO3a pathway and satellite cell function in chronic kidney disease. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021 (PubMed: 33405354) [IF=5.3]

Application: WB    Species: Mouse    Sample: C2C12 myoblasts

FIGURE 5 FMN inhibits myostatin-mediated dephosphorylation on the PI3K/Akt/FoxO3a signalling pathway in the muscle of CKD rats and C2C12 myotubes. (A) Protein levels of p-PI3K, PI3K, p-Akt, Akt, p-FoxO3a and FoxO3a in gastrocnemius muscle analysed using Western blotting (n = 3/group). (B) Protein levels of MAFbx and MuRF-1 in gastrocnemius muscles analysed using Western blotting (n = 3/ group). (C) C2C12 myotubes were treated with FMN (50 μmol/L) in the presence or absence of TNF-α (40 ng/mL) for 48 h following 24 h of incubation with si-myostatin or si-NC. The myotubes were divided into four groups: si-NC, si-NC + TNF-α, si-NC + TNF-α + FMN (50 μmol/L) and si-myostatin + TNF-α. Protein levels of p-PI3K, PI3K, p-Akt, Akt, p-FoxO3a and FoxO3a in C2C12 myotubes (n = 3/ group). (D) Protein levels of MAFbx and MuRF-1 in C2C12 myotubes (n = 3/group). (E) Myostatin OE transfection was used to overexpress myostatin in C2C12 myotubes, and they were incubated with FMN (50 μmol/L) and TNF-α for another 48 h. The myotubes were divided into four groups: vector NC, vector NC + TNF-α, vector NC + TNF-α + FMN (50 μmol/L) and myostatin OE + TNF-α + FMN (50 μmol/L). The protein levels of p-PI3K, PI3K, p-Akt, Akt, p-FoxO3a and FoxO3a in the myotubes were analysed using Western blotting. (F) Protein levels of MAFbx and MuRF1 in C2C12 myotubes analysed by Western blotting (n = 3/group). *P < .05, **P < .01
9). Study on the effects and mechanisms of Wenzhong Bushen Formula in improving ovarian reserve decline in mice based on network pharmacology. Journal of ethnopharmacology, 2024 (PubMed: 38218503) [IF=4.8]
10). DNA damage induced PARP-1 overactivation confers paclitaxel-induced neuropathic pain by regulating mitochondrial oxidative metabolism. CNS neuroscience & therapeutics, 2024 (PubMed: 39215404) [IF=4.8]

Application: WB    Species: Rat    Sample: spinal cord

FIGURE 7. Effect of paclitaxel injection on deacetylase activity of SIRT3 in the DRGs and spinal cord in rats. (A–D) The ratios of p‐FoxO3a/FoxO3a were significantly upregulated in the DRGs and spinal cord after paclitaxel injection. One‐way ANOVA followed by Bonferroni post hoc test, *p 
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