Phospho-IRAK4 (Thr345/Ser346) Antibody - #DF7567

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产品: 磷酸化 IRAK4 (Thr345/Ser346) 抗体
货号: DF7567
描述: Rabbit polyclonal antibody to Phospho-IRAK4 (Thr345/Ser346)
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: Q9NWZ3
RRID: AB_2841060

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 100ul RMB¥ 2400 现货
 200ul RMB¥ 3200 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-IRAK4 (Thr345/Ser346) Antibody detects endogenous levels of IRAK4 only when phosphorylated at Thr345/Ser346.
RRID:
AB_2841060
引用格式: Affinity Biosciences Cat# DF7567, RRID:AB_2841060.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

IL-1 receptor-associated kinase 4; Interleukin 1 receptor associated kinase 4 mutant form 1; Interleukin-1 receptor-associated kinase 4; Interleukin1 receptor associated kinase 4; IPD1; IRAK 4; IRAK-4; IRAK4; IRAK4 mutated form 1; IRAK4_HUMAN; LOC 51135; NY REN 64; NY REN 64 antigen; NY-REN-64; REN64; Renal carcinoma antigen NY-REN-64;

抗原和靶标

免疫原:

A synthesized peptide derived from human IRAK4 around the phosphorylation site of Thr345/Ser346.

基因/基因ID:
IRAK4(51135)

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

文献引用

1). Discovery of Novel MyD88 Inhibitor A5S to Alleviate Acute Lung Injury with Favorable Drug-like Properties. Journal of medicinal chemistry, 2024 (PubMed: 39644263) [IF=7.3]
2). The novel mechanism of human norovirus induced diarrhea: Activation of PKD2 caused by HuNoVs destroyed AQP3 expression through AP2γ in intestinal epithelial cells. Life sciences, 2024 (PubMed: 38103725) [IF=5.2]
3). Artemisinin attenuates perinatal inflammation and consequent oxidative stress in oligodendrocyte precursor cells by inhibiting IRAK-4 and IRAK-1. International immunopharmacology, 2024 (PubMed: 39293313) [IF=4.8]

Application: WB    Species: Rat    Sample: OPCs

Fig. 4. LPS-induced activation of the IRAK-4/IRAK-1/NF-κB signalling pathway in OPCs was inhibited by ART. a, b, c, d Western blotting was used to quantify the protein levels of p-IRAK-4, IRAK-4, p-IRAK-4/IRAK-4, p-IRAK-1, IRAK-1, p-IRAK-1/IRAK-1, phospho-NF-kB p65, NF-κB p65, and phospho-NF-kB p65/NF-κB p65 in 4 groups (the Ctrl, ART, LPS, and LPS + ART groups). The data are expressed as the means ± SEMs (n = 6/group) (*p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001).

Application: IF/ICC    Species: Rat    Sample: OPCs

Fig. 5. ART reduces NF-κB nuclear aggregation after LPS stimulation. a, b, c Immunofluorescence was used to compare the relative fluorescence of p-IRAK-4 (red) and p-IRAK-1 (red) in OPCs across several groups (the Ctrl, ART, LPS, and LPS + ART groups), and the proportion (%) of nuclear NF-κB p65 (red) was determined. DAPI staining is shown in blue. Scale bar = 50 μm. The data are presented as the means ± SEMs (n = 6). ****P < 0.0001 versus the indicated groups.
4). Disulfiram attenuates cell and tissue damage and blood‒brain barrier dysfunction after intracranial haemorrhage by inhibiting the classical pyroptosis pathway. Scientific reports, 2024 (PubMed: 39300102) [IF=3.8]
5). Pharmacological inhibition of IRAK1 attenuates colitis‐induced tumorigenesis in mice by inhibiting the inflammatory response and epithelial–mesenchymal transition. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, 2021 (PubMed: 34273909) [IF=3.2]

Application: WB    Species: Human    Sample: HCT‐116 cells

FIGURE 4 IRAK1/4 inhibitor treatment inhibits epithelial–mesenchymal transition (EMT) in mice by reducing TLR/IL‐1R‐mediated NF‐κB activation. (A) IRAK1/4 inhibitor treatment decreased the expression of phosphorylated IRAK4, IRAK1, and P65. (B) IRAK1/4 inhibitor treatment inhibits EMT induced by exposure to azoxymethane/dextran sodium sulfate (AOM/DSS). Values are expressed as mean±SD. # Significantly different compared with control, p<0.05; *significantly different compared with model, p <0.05

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