IRF7 Antibody - #DF7503

Fig. 7 Inhibition of IRF7 or IRF3 can alleviate lung injury and macrophage pyroptosis. A–C We induced SAP in wild-type mice after tail vein injection of si-IRF7 (n = 6 per group). HE staining and IHC of IRF7 in lung tissue (A). Score of lung injury and IHC (B). The levels of IL-1β, IL-6 and TNF-α in serum (C). D–F We induced SAP in wild-type mice after tail vein injection of si-IRF3 (n = 6 per group). HE staining and IHC of IRF3 in lung tissue (D). Score of lung injury and IHC (E). The levels of IL-1β, IL-6 and TNF-α in serum (F). G Flow cytometry was used to visualize pyroptosis of alveolar macrophages

FIGURE 5 Prolonged phagocytosis facilitates STING (stimulator of interferon genes) activation and phenotypic shift in microglia and macrophages. A mixture of brain-derived danger-associated molecular patterns (DAMPs) was extracted from naïve mouse brains and administered to primary macrophage and microglia for short-term (24 h) and prolonged (48 h) phagocytosis. Following treatment, we assessed the inflammatory properties and STING activation in microglia and macrophages. Representative plots and the percentage of TNF-α+/IL-10+ macrophages (A) and microglia (B) in control, short-term, and prolonged phagocytosis groups. Experiments were conducted four times. *p 

Fig. 7| mRNA and protein expression of the TLR7 signaling pathway in lung tissue. (a) Expression of TLR7, MyD88, TRAF6, IRF7, and NF-κB mRNA in lung tissue (n = 5). (b) Western blot of the protein expression of TLR7, MyD88, TRAF6, IRF7, P-IRF7, and IκBα in lung tissue (n = 3). (c) Quantification of TLR7, MyD88, TRAF6, P-IRF7/IRF7, and IκBα protein expression relative to β-actin. Data are presented as the means ± SD. *P < 0.05, **P < 0.01 vs the model group. #P < 0.05, ##P < 0.01 vs the control group

Fig. 2ANXA2 regulated inflammatory gene mRNA and protein expression in HK2 cells. A Top ten GO biological processes terms enriched by upregulated DEGs in shANXA2 cells vs shCtrl cells. B Top ten KEGG functional pathways enriched by upregulated DEGs in shANXA2 cells vs shCtrl cells. C Top ten KEGG functional pathways enriched by downregulated DEGs in shANXA2 cells vs shCtrl cells. D Validation of mRNA expression of CCL5, IFI6, IFI44, IFITM1,and LTB by qRT-PCR assay. E Validation of mRNA expression of IRF7 and ISG15 by qRT-PCR assay. F Representative images showing protein levels of ANXA2, CCL5, IFI6, IFI44, IFITM1, LTB, IRF7 and ISG15 in LV-shANXA2 group vs LV-shCtrl group. Results are represented as mean ± SD.(n = 3,* P