Phospho-Raptor (Ser863) Antibody - #AF8506

Fig. 2 Upregulation of mTORC1 and downregulation of mTORC2 in the GDM fetal lungs. A The protein expression of TRAF2, OTUD7B, p-mTOR, mTOR, and GβL in the control and GDM fetal lung tissues. B The determined protein levels of p-Sin1, Sin1, p-Rictor, Rictor, p-Raptor, Raptor, p-Akt, and Akt in the control and GDM fetal lung tissues. C The relative protein levels of TRAF2, OTUD7B, p-mTOR, mTOR, and GL in the control and GDM fetal lung tissues. D The relative protein levels of p-Sin1, Sin1, p-Rictor, Rictor, p-Raptor, Raptor, p-Akt, and Akt in the control and GDM fetal lung tissues (n = 3 in triplicate, **p 

Fig. 6. Validation of the proteins in the regulation of the PI38-Akt signaling pathway and mitochondrial activity. A) Representative Western blotting images of SREBP-1, p-Raptor, Raptor, p-AMPK, AMPK, p-Akt, Akt, FoxO1. B) Relative expression of srebp-1 **P < 0.01. C) Relative expression of mTORC1. D) PI3K-Akt signaling pathway. E) Grey value analyses of SREBP-1, p-Raptor, p-AMPK, p-Akt, and FoxO1 *P < 0.05, n = 3 rats per group. F) Directionality of the changes in mTORC1 and PI3K-Akt signaling pathway, frame with red background: proteins up-regulated in MDL, frame with claybank background: proteins down-regulated in MDL, green arrows: proteins regulated by LCF. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)