Phospho-IRF7 (Ser477) Antibody - #AF8486

FIGURE 5 Prolonged phagocytosis facilitates STING (stimulator of interferon genes) activation and phenotypic shift in microglia and macrophages. A mixture of brain-derived danger-associated molecular patterns (DAMPs) was extracted from naïve mouse brains and administered to primary macrophage and microglia for short-term (24 h) and prolonged (48 h) phagocytosis. Following treatment, we assessed the inflammatory properties and STING activation in microglia and macrophages. Representative plots and the percentage of TNF-α+/IL-10+ macrophages (A) and microglia (B) in control, short-term, and prolonged phagocytosis groups. Experiments were conducted four times. *p 

Fig. 7| mRNA and protein expression of the TLR7 signaling pathway in lung tissue. (a) Expression of TLR7, MyD88, TRAF6, IRF7, and NF-κB mRNA in lung tissue (n = 5). (b) Western blot of the protein expression of TLR7, MyD88, TRAF6, IRF7, P-IRF7, and IκBα in lung tissue (n = 3). (c) Quantification of TLR7, MyD88, TRAF6, P-IRF7/IRF7, and IκBα protein expression relative to β-actin. Data are presented as the means ± SD. *P < 0.05, **P < 0.01 vs the model group. #P < 0.05, ##P < 0.01 vs the control group