产品: 磷酸化 p70 S6 Kinase (Ser371) 抗体
货号: AF3227
描述: Rabbit polyclonal antibody to Phospho-p70 S6 Kinase (Ser371)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 70kDa; 59kD(Calculated).
蛋白号: P23443
RRID: AB_2834328

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
Phospho-p70 S6 Kinase (Ser371) Antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at Serine 371.
RRID:
AB_2834328
引用格式: Affinity Biosciences Cat# AF3227, RRID:AB_2834328.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

70 kDa ribosomal protein S6 kinase 1; KS6B1_HUMAN; p70 alpha; P70 beta 1; p70 ribosomal S6 kinase alpha; p70 ribosomal S6 kinase beta 1; p70 S6 kinase alpha; P70 S6 Kinase; p70 S6 kinase, alpha 1; p70 S6 kinase, alpha 2; p70 S6K; p70 S6K-alpha; p70 S6KA; p70(S6K) alpha; p70(S6K)-alpha; p70-alpha; p70-S6K 1; p70-S6K; P70S6K; P70S6K1; p70S6Kb; PS6K; Ribosomal protein S6 kinase 70kDa polypeptide 1; Ribosomal protein S6 kinase beta 1; Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; RPS6KB1; S6K; S6K-beta-1; S6K1; Serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; STK14A;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P23443 KS6B1_HUMAN:

Widely expressed.

描述:
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein.
序列:
MRRRRRRDGFYPAPDFRDREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Dog
100
Chicken
100
Rabbit
100
Bovine
92
Sheep
92
Xenopus
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P23443 作为底物

Site PTM Type Enzyme
S40 Phosphorylation
S53 Phosphorylation Q9HC98 (NEK6)
K85 Ubiquitination
K99 Ubiquitination
K104 Ubiquitination
K118 Ubiquitination
S243 Phosphorylation
T248 Phosphorylation
T250 Phosphorylation
T252 Phosphorylation P42336 (PIK3CA) , O15530 (PDPK1)
T256 Phosphorylation
S278 Phosphorylation
K304 Acetylation
K364 Ubiquitination
K370 Ubiquitination
S371 Phosphorylation
T389 Phosphorylation
T390 Phosphorylation P42345 (MTOR)
S394 Phosphorylation P06493 (CDK1) , P42345 (MTOR)
T399 Phosphorylation
S403 Phosphorylation Q9HC98 (NEK6)
T412 Phosphorylation O94768 (STK17B) , O15530 (PDPK1) , Q8TDX7 (NEK7) , Q9HC98 (NEK6) , P42336 (PIK3CA) , P42345 (MTOR) , P23443 (RPS6KB1)
Y413 Phosphorylation
S417 Phosphorylation
S421 Phosphorylation
K425 Ubiquitination
S427 Phosphorylation
S434 Phosphorylation P06493 (CDK1) , P42345 (MTOR) , P45984 (MAPK9) , P27361 (MAPK3) , P45983 (MAPK8) , P28482 (MAPK1)
S441 Phosphorylation
T444 Phosphorylation P06493 (CDK1) , P28482 (MAPK1)
S447 Phosphorylation P28482 (MAPK1) , P06493 (CDK1) , P42345 (MTOR)
K450 Ubiquitination
S452 Phosphorylation
S462 Phosphorylation
T470 Phosphorylation
K516 Acetylation
R522 Methylation

翻译修饰 - P23443 作为激酶

Substrate Site Source
O00418 (EEF2K) S366 Uniprot
O60825 (PFKFB2) S466 Uniprot
O94763 (URI1) S372 Uniprot
P03372 (ESR1) S167 Uniprot
P04637 (TP53) S392 Uniprot
P04792 (HSPB1) S15 Uniprot
P04792 (HSPB1) S78 Uniprot
P04792 (HSPB1) S82 Uniprot
P08151 (GLI1) S84 Uniprot
P10636-8 (MAPT) T212 Uniprot
P10636-8 (MAPT) S214 Uniprot
P10636-8 (MAPT) S262 Uniprot
P23443 (RPS6KB1) T412 Uniprot
P23588-1 (EIF4B) S422 Uniprot
P27708 (CAD) S1859 Uniprot
P35568 (IRS1) S270 Uniprot
P35568 (IRS1) S307 Uniprot
P35568 (IRS1) S527 Uniprot
P35568 (IRS1) S636 Uniprot
P35568 (IRS1) S1101 Uniprot
P42345 (MTOR) T2446 Uniprot
P42345 (MTOR) S2448 Uniprot
P49841 (GSK3B) S9 Uniprot
P62753 (RPS6) S235 Uniprot
P62753 (RPS6) S236 Uniprot
P62753 (RPS6) S240 Uniprot
P62753 (RPS6) S244 Uniprot
P62753 (RPS6) S247 Uniprot
P78371 (CCT2) S260 Uniprot
Q05195 (MXD1) S145 Uniprot
Q06787 (FMR1) S500 Uniprot
Q09161 (NCBP1) S7 Uniprot
Q09161 (NCBP1) T21 Uniprot
Q09161 (NCBP1) S22 Uniprot
Q15831-1 (STK11) S428 Uniprot
Q16873 (LTC4S) S36 Uniprot
Q53EL6 (PDCD4) S67 Uniprot
Q6R327 (RICTOR) T1135 Uniprot
Q8TB45 (DEPTOR) S286 Uniprot
Q8TB45 (DEPTOR) S287 Uniprot
Q8TB45 (DEPTOR) S291 Uniprot
Q92519 (TRIB2) S83 Uniprot
Q92934 (BAD) S99 Uniprot
Q9BY77 (POLDIP3) S383 Uniprot
Q9BY77 (POLDIP3) S385 Uniprot
Q9BYV9 (BACH2) S521 Uniprot
Q9H3D4 (TP63) S477 Uniprot
Q9H3D4 (TP63) T491 Uniprot
Q9H3D4 (TP63) S560 Uniprot
Q9UN36 (NDRG2) S332 Uniprot
Q9UN36 (NDRG2) S350 Uniprot

研究背景

功能:

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR. Following activation by mTORC1, phosphorylates EPRS and thereby plays a key role in fatty acid uptake by adipocytes and also most probably in interferon-gamma-induced translation inhibition.

翻译修饰:

Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (By similarity). Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.

细胞定位:

Cell junction>Synapse>Synaptosome. Mitochondrion outer membrane. Mitochondrion.
Note: Colocalizes with URI1 at mitochondrion.

Nucleus. Cytoplasm.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed.

亚基结构:

Interacts with PPP1R9A/neurabin-1 (By similarity). Interacts with RPTOR. Interacts with IRS1. Interacts with EIF3B and EIF3C. Interacts with TRAF4. Interacts with POLDIP3. Interacts (via N-terminus) with IER5.

蛋白家族:

The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.

The TOS (TOR signaling) motif is essential for activation by mTORC1.

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

文献引用

1). Melatonin Repairs Osteoporotic Bone Defects in Iron-Overloaded Rats through PI3K/AKT/GSK-3β/P70S6k Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2023 (PubMed: 36703914)

Application: WB    Species: Mouse    Sample: MC3T3-E1 cells

Figure 5 Showing how MT uses the PI3K/AKT/GSK-3β/P70S6k signaling pathway to treat iron-overloaded MC3T3-E1 cells. (a) All mRNA expression levels in MC3T3-E1 cells were determined by using RT-PCR for p-PI3K, p-GSK-3β, p-AKT, and p-P70S6k. (b) Western blotting was used to analyze the expression of PI3K, AKT, GSK-3β, P70S6k, p-PI3K, p-AKT, p-GSK-3β, p-P70S6k, and β-actin proteins in MC3T3-E1 cells across treatment groups. MT stands for melatonin group; FAC+MT+LY294002 stands for the ferric ammonium citrate+melatonin+LY294002 group; PI3K stands for phosphatidylinositol kinase; p-PI3K stands for phosphorylated phosphatidylinositol kinase; AKT stands for protein kinase B; p-AKT stands for phosphorylated protein kinase B; p-GSK-3β stands for phosphorylated glycogen synthase kinase-3β; GSK-3β stands for glycogen synthase kinase-3β; P70S6 stands for ribosomal S6 protein kinase; p-P70S6k stands for phosphorylated ribosomal S6 protein kinase;

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