MARK4 Antibody - #AF0693

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产品: MARK4 抗体
货号: AF0693
描述: Rabbit polyclonal antibody to MARK4
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Dog
分子量: 82kDa; 83kD(Calculated).
蛋白号: Q96L34
RRID: AB_2834302

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产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
MARK4 Antibody detects endogenous levels of total MARK4.
RRID:
AB_2834302
引用格式: Affinity Biosciences Cat# AF0693, RRID:AB_2834302.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ90097; KIAA1860; MAP / microtubule affinity regulating kinase 4; MAP / microtubule affinity regulating kinase 4L; MAP/microtubule affinity regulating kinase 4; MAP/microtubule affinity regulating kinase like 1; MAP/microtubule affinity-regulating kinase 4; MAP/microtubule affinity-regulating kinase-like 1; MARK 4; MARK 4L; MARK L1; MARK4; MARK4 serine / threonine protein kinase; MARK4 serine/threonine protein kinase; MARK4_HUMAN; MARK4S; MARKL 1; MARKL1; Nbla00650; Putative protein product of Nbla00650;

抗原和靶标

免疫原:
Uniprot:

Q96L34(MARK4_HUMAN) >>Visit HPA database.

基因/基因ID:
MARK4(57787)
表达:
Q96L34 MARK4_HUMAN:

Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945).

描述:
MARK4 a member of the CAMKL family of protein kinases. Normally expressed in neural progenitors and is re-expressed in gliomas.
序列:
MSSRTVLAPGNDRNSDTHGTLGSGRSSDKGPSWSSRSLGARCRNSIASCPEEQPHVGNYRLLRTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVKLFEVIETEKTLYLVMEYASAGEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHRDLKAENLLLDAEANIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDIWSLGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVPFYMSTDCESILRRFLVLNPAKRCTLEQIMKDKWINIGYEGEELKPYTEPEEDFGDTKRIEVMVGMGYTREEIKESLTSQKYNEVTATYLLLGRKTEEGGDRGAPGLALARVRAPSDTTNGTSSSKGTSHSKGQRSSSSTYHRQRRHSDFCGPSPAPLHPKRSPTSTGEAELKEERLPGRKASCSTAGSGSRGLPPSSPMVSSAHNPNKAEIPERRKDSTSTPNNLPPSMMTRRNTYVCTERPGAERPSLLPNGKENSSGTPRVPPASPSSHSLAPPSGERSRLARGSTIRSTFHGGQVRDRRAGGGGGGGVQNGPPASPTLAHEAAPLPAGRPRPTTNLFTKLTSKLTRRVADEPERIGGPEVTSCHLPWDQTETAPRLLRFPWSVKLTSSRPPEALMAALRQATAAARCRCRQPQPFLLACLHGGAGGPEPLSHFEVEVCQLPRPGLRGVLFRRVAGTALAFRTLVTRISNDLEL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
60
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q96L34 作为底物

Site PTM Type Enzyme
S15 Phosphorylation
T17 Phosphorylation
T20 Phosphorylation
S23 Phosphorylation
S26 Phosphorylation
S27 Phosphorylation
K29 Ubiquitination
S45 Phosphorylation
S48 Phosphorylation
R60 Methylation
T64 Phosphorylation
K67 Ubiquitination
K88 Ubiquitination
K92 Ubiquitination
K102 Ubiquitination
K111 Ubiquitination
K183 Ubiquitination
T214 Phosphorylation Q15831 (STK11)
S218 Phosphorylation
K230 Ubiquitination
K297 Ubiquitination
K306 Ubiquitination
K320 Ubiquitination
K356 Ubiquitination
S391 Phosphorylation
T393 Phosphorylation
T394 Phosphorylation
S404 Phosphorylation
S423 Phosphorylation
S438 Phosphorylation
T440 Phosphorylation
S441 Phosphorylation
T442 Phosphorylation
S458 Phosphorylation
S496 Phosphorylation
S504 Phosphorylation
T507 Phosphorylation
T511 Phosphorylation
S543 Phosphorylation
S545 Phosphorylation
S546 Phosphorylation
R561 Methylation
S563 Phosphorylation
T564 Phosphorylation
S567 Phosphorylation
T568 Phosphorylation
S594 Phosphorylation
K622 Ubiquitination

翻译修饰 - Q96L34 作为激酶

Substrate Site Source
Q96L34-2 (MARK4) S26 Uniprot
Q96L34-2 (MARK4) T214 Uniprot

研究背景

功能:

Serine/threonine-protein kinase. Phosphorylates the microtubule-associated protein MAPT/TAU. Also phosphorylates the microtubule-associated proteins MAP2 and MAP4. Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles. Required for the initiation of axoneme extension during cilium assembly. Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro. Plays a role in cell cycle progression, specifically in the G1/S checkpoint. Reduces neuronal cell survival. Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation.

翻译修饰:

Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.

Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylated throughout the cell cycle.

细胞定位:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center. Cytoplasm>Cytoskeleton>Cilium basal body. Cytoplasm>Cytoskeleton>Cilium axoneme. Cytoplasm. Cell projection>Dendrite.
Note: Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous. Isoform 2 is brain-specific. Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen.

亚基结构:

Interacts with MAPT/TAU. Interacts with gamma-tubulin. Interacts with ODF2. Interacts with USP9X. Interacts with YWHAQ.

蛋白家族:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

文献引用

1). Microtubule affinity regulating kinase 4 promoted activation of the NLRP3 inflammasome-mediated pyroptosis in periodontitis. Journal of Oral Microbiology, 2022 (PubMed: 34992737) [IF=4.5]

Application: IHC    Species: Human    Sample: gingival tissues

Figure 1. Increased MARK4 and the NLRP3 inflammasome activation in inflamed gingival tissues (a) Gene transcription in the healthy (n = 10) and inflamed (n = 10) gingiva was measured by real time qPCR. (b and c) Samples from healthy (n = 6) and inflamed gingiva (n = 6) were stained by the immunolohistological chemistry. The scale bar represents 50 µm. (d) Protein levels in the gingival tissue from chronic periodontitis (CP, n = 3) or normal control (NC, n = 3) were evaluated by Western blot. *p < 0.05; **p < 0.01; ***p < 0.001.

Application: WB    Species: Human    Sample: gingival tissues

Figure 1. Increased MARK4 and the NLRP3 inflammasome activation in inflamed gingival tissues (a) Gene transcription in the healthy (n = 10) and inflamed (n = 10) gingiva was measured by real time qPCR. (b and c) Samples from healthy (n = 6) and inflamed gingiva (n = 6) were stained by the immunolohistological chemistry. The scale bar represents 50 µm. (d) Protein levels in the gingival tissue from chronic periodontitis (CP, n = 3) or normal control (NC, n = 3) were evaluated by Western blot. *p < 0.05; **p < 0.01; ***p < 0.001.

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