产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0579, RRID:AB_2834246.
展开/折叠
DM 1; DM; DM kinase; DM protein kinase; DM-kinase; DM1; DM1 protein kinase; DM1PK; DMK; DMPK; DMPK_HUMAN; Dystrophia myotonica 1; Dystrophia myotonica protein kinase; MDPK; MT PK; MT-PK; Myotonic dystrophy associated protein kinase; Myotonic dystrophy protein kinase; Myotonin protein kinase A; Myotonin protein kinase; Myotonin-protein kinase; Thymopoietin homolog;
抗原和靶标
A synthesized peptide derived from human DMPK, corresponding to a region within N-terminal amino acids.
Most isoforms are expressed in many tissues including heart, skeletal muscle, liver and brain, except for isoform 2 which is only found in the heart and skeletal muscle, and isoform 14 which is only found in the brain, with high levels in the striatum, cerebellar cortex and pons.
- Q09013 DMPK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSAEVRLRRLQQLVLDPGFLGLEPLLDLLLGVHQELGASELAQDKYVADFLQWAEPIVVRLKEVRLQRDDFEILKVIGRGAFSEVAVVKMKQTGQVYAMKIMNKWDMLKRGEVSCFREERDVLVNGDRRWITQLHFAFQDENYLYLVMEYYVGGDLLTLLSKFGERIPAEMARFYLAEIVMAIDSVHRLGYVHRDIKPDNILLDRCGHIRLADFGSCLKLRADGTVRSLVAVGTPDYLSPEILQAVGGGPGTGSYGPECDWWALGVFAYEMFYGQTPFYADSTAETYGKIVHYKEHLSLPLVDEGVPEEARDFIQRLLCPPETRLGRGGAGDFRTHPFFFGLDWDGLRDSVPPFTPDFEGATDTCNFDLVEDGLTAMVSGGGETLSDIREGAPLGVHLPFVGYSYSCMALRDSEVPGPTPMELEAEQLLEPHVQAPSLEPSVSPQDETAEVAVPAAVPAAEAEAEVTLRELQEALEEEVLTRQSLSREMEAIRTDNQNFASQLREAEARNRDLEAHVRQLQERMELLQAEGATAVTGVPSPRATDPPSHLDGPPAVAVGQCPLVGPGPMHRRHLLLPARVPRPGLSEALSLLLFAVVLSRAAALGCIGLVAHAGQLTAVWRRPGAARAP
翻译修饰 - Q09013 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| T533 | Phosphorylation | Uniprot | |
| T536 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| R579 | Methylation | Uniprot |
翻译修饰 - Q09013 作为激酶
研究背景
Non-receptor serine/threonine protein kinase which is necessary for the maintenance of skeletal muscle structure and function. May play a role in myocyte differentiation and survival by regulating the integrity of the nuclear envelope and the expression of muscle-specific genes. May also phosphorylate PPP1R12A and inhibit the myosin phosphatase activity to regulate myosin phosphorylation. Also critical to the modulation of cardiac contractility and to the maintenance of proper cardiac conduction activity probably through the regulation of cellular calcium homeostasis. Phosphorylates PLN, a regulator of calcium pumps and may regulate sarcoplasmic reticulum calcium uptake in myocytes. May also phosphorylate FXYD1/PLM which is able to induce chloride currents. May also play a role in synaptic plasticity.
Phosphorylated. Autophosphorylates. Phosphorylation by RAF1 may result in activation of DMPK.
Proteolytic processing of the C-terminus may remove the transmembrane domain and release the kinase from membranes stimulating its activity.
Endoplasmic reticulum membrane>Single-pass type IV membrane protein>Cytoplasmic side. Nucleus outer membrane>Single-pass type IV membrane protein>Cytoplasmic side. Mitochondrion outer membrane>Single-pass type IV membrane protein. Sarcoplasmic reticulum membrane. Cell membrane. Cytoplasm>Cytosol.
Note: Localizes to sarcoplasmic reticulum membranes of cardiomyocytes.
Mitochondrion membrane.
Mitochondrion membrane.
Most isoforms are expressed in many tissues including heart, skeletal muscle, liver and brain, except for isoform 2 which is only found in the heart and skeletal muscle, and isoform 14 which is only found in the brain, with high levels in the striatum, cerebellar cortex and pons.
Homodimer; homodimerization stimulates the kinase activity. Interacts with HSPB2; may enhance DMPK kinase activity. Interacts with PLN; phosphorylates PLN. May interact with RAC1; may regulate DMPK kinase activity. Interacts with LMNA; may regulate nuclear envelope stability.
The coiled coil domain is required for homodimerization and regulates the enzymatic activity.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
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