RPL31 Antibody - #AF0854

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产品: RPL31 抗体
货号: AF0854
描述: Rabbit polyclonal antibody to RPL31
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 14kDa; 14kD(Calculated).
蛋白号: P62899
RRID: AB_2834226

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实验方案
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IHC Protocol
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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
RPL31 Antibody detects endogenous levels of total RPL31.
RRID:
AB_2834226
引用格式: Affinity Biosciences Cat# AF0854, RRID:AB_2834226.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

60S ribosomal protein L31; L31; MGC88191; OTTHUMP00000202933; OTTHUMP00000202940; OTTHUMP00000202941; OTTHUMP00000202942; OTTHUMP00000202943; Ribosomal protein L31; RL31_HUMAN; rpl31;

抗原和靶标

免疫原:
Uniprot:

P62899(RL31_HUMAN) >>Visit HPA database.

基因/基因ID:
RPL31(6160)
描述:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L31E family of ribosomal proteins. It is located in the cytoplasm. Higher levels of expression of this gene in familial adenomatous polyps compared to matched normal tissues have been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
序列:
MAPAKKGGEKKKGRSAINEVVTREYTINIHKRIHGVGFKKRAPRALKEIRKFAMKEMGTPDVRIDTRLNKAVWAKGIRNVPYRIRVRLSRKRNEDEDSPNKLYTLVTYVPVTTFKNLQTVNVDEN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P62899 作为底物

Site PTM Type Enzyme
M1 Acetylation
R14 Methylation
S15 Phosphorylation
Y25 Phosphorylation
T26 Phosphorylation
K31 Methylation
K31 Ubiquitination
K39 Acetylation
K39 Methylation
K39 Ubiquitination
K55 Acetylation
K55 Ubiquitination
R63 Methylation
K70 Ubiquitination
K75 Acetylation
K75 Ubiquitination
S89 Phosphorylation
S98 Phosphorylation
K101 Ubiquitination
Y103 Phosphorylation
T104 Phosphorylation
T107 Phosphorylation
Y108 Phosphorylation
T113 Phosphorylation
K115 Methylation
K115 Ubiquitination
T119 Phosphorylation

研究背景

蛋白家族:

Belongs to the eukaryotic ribosomal protein eL31 family.

研究领域

· Genetic Information Processing > Translation > Ribosome.

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