SIX6 Antibody - #AF0724

(2)  
产品: SIX6 抗体
货号: AF0724
描述: Rabbit polyclonal antibody to SIX6
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 28kDa; 28kD(Calculated).
蛋白号: O95475
RRID: AB_2834201

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
SIX6 Antibody detects endogenous levels of total SIX6.
RRID:
AB_2834201
引用格式: Affinity Biosciences Cat# AF0724, RRID:AB_2834201.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Homeobox protein SIX6; Homeodomain protein OPTX2; MCOPCT 2; MCOPCT2; Optic homeobox 2; OPTX 2; OPTX2; Sine oculis homeobox homolog 6; SIX 6; Six 9; SIX homeobox 6; Six6; SIX6_HUMAN; Six9;

抗原和靶标

免疫原:
Uniprot:

O95475(SIX6_HUMAN) >>Visit HPA database.

基因/基因ID:
SIX6(4990)
表达:
O95475 SIX6_HUMAN:

Expressed in the developing and adult retina. Also expressed in the hypothalamic and the pituitary regions.

描述:
SIX6 May be involved in eye development. Belongs to the SIX/Sine oculis homeobox family. Note: This description may include information from UniProtKB.
序列:
MFQLPILNFSPQQVAGVCETLEESGDVERLGRFLWSLPVAPAACEALNKNESVLRARAIVAFHGGNYRELYHILENHKFTKESHAKLQALWLEAHYQEAEKLRGRPLGPVDKYRVRKKFPLPRTIWDGEQKTHCFKERTRHLLREWYLQDPYPNPSKKRELAQATGLTPTQVGNWFKNRRQRDRAAAAKNRLQQQVLSQGSGRALRAEGDGTPEVLGVATSPAASLSSKAATSAISITSSDSECDI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Bovine
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O95475 作为底物

Site PTM Type Enzyme
S10 Phosphorylation
K49 Ubiquitination
Y96 Phosphorylation
K112 Ubiquitination
K131 Ubiquitination
K136 Ubiquitination
Y147 Phosphorylation
Y152 Phosphorylation
S156 Phosphorylation
S198 Phosphorylation
S201 Phosphorylation
T212 Phosphorylation
T220 Phosphorylation
S221 Phosphorylation
S225 Phosphorylation
S227 Phosphorylation
S228 Phosphorylation

研究背景

功能:

May be involved in eye development.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the developing and adult retina. Also expressed in the hypothalamic and the pituitary regions.

亚基结构:

Interacts with TLE4 and TLE5.

蛋白家族:

Belongs to the SIX/Sine oculis homeobox family.

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