产品: MAP3K6 抗体
货号: AF0461
描述: Rabbit polyclonal antibody to MAP3K6
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Horse, Rabbit, Dog
分子量: 142kDa; 143kD(Calculated).
蛋白号: O95382
RRID: AB_2834199

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Horse(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
MAP3K6 Antibody detects endogenous levels of total MAP3K6.
RRID:
AB_2834199
引用格式: Affinity Biosciences Cat# AF0461, RRID:AB_2834199.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Apoptosis signal regulating kinase 2; Apoptosis signal-regulating kinase 2; ASK2; EC 2.7.11.25; M3K6_HUMAN; MAP3K6; MAPKKK6; MEKK6; MGC125653; MGC20114; Mitogen-activated protein kinase kinase kinase 6;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
a member of the STE11 protein kinase family. Activates JNK, but not ERK or p38. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported
序列:
MAGPCPRSGAERAGSCWQDPLAVALSRGRQLAAPPGRGCARSRPLSVVYVLTREPQPGLEPREGTEAEPLPLRCLREACAQVPRPRPPPQLRSLPFGTLELGDTAALDAFYNADVVVLEVSSSLVQPSLFYHLGVRESFSMTNNVLLCSQADLPDLQALREDVFQKNSDCVGSYTLIPYVVTATGRVLCGDAGLLRGLADGLVQAGVGTEALLTPLVGRLARLLEATPTDSCGYFRETIRRDIRQARERFSGPQLRQELARLQRRLDSVELLSPDIIMNLLLSYRDVQDYSAIIELVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDRAKALSVLLPLVQLEGSVAPDLYCMCGRIYKDMFFSSGFQDAGHREQAYHWYRKAFDVEPSLHSGINAAVLLIAAGQHFEDSKELRLIGMKLGCLLARKGCVEKMQYYWDVGFYLGAQILANDPTQVVLAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTPEPPGGPPRRAHFWLHFLLQSCQPFKTACAQGDQCLVLVLEMNKVLLPAKLEVRGTDPVSTVTLSLLEPETQDIPSSWTFPVASICGVSASKRDERCCFLYALPPAQDVQLCFPSVGHCQWFCGLIQAWVTNPDSTAPAEEAEGAGEMLEFDYEYTETGERLVLGKGTYGVVYAGRDRHTRVRIAIKEIPERDSRFSQPLHEEIALHRRLRHKNIVRYLGSASQGGYLKIFMEEVPGGSLSSLLRSVWGPLKDNESTISFYTRQILQGLGYLHDNHIVHRDIKGDNVLINTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGRPPFHELGSPQAAMFQVGMYKVHPPMPSSLSAEAQAFLLRTFEPDPRLRASAQTLLGDPFLQPGKRSRSPSSPRHAPRPSDAPSASPTPSANSTTQSQTFPCPQAPSQHPPSPPKRCLSYGGTSQLRVPEEPAAEEPASPEESSGLSLLHQESKRRAMLAAVLEQELPALAENLHQEQKQEQGARLGRNHVEELLRCLGAHIHTPNRRQLAQELRALQGRLRAQGLGPALLHRPLFAFPDAVKQILRKRQIRPHWMFVLDSLLSRAVRAALGVLGPEVEKEAVSPRSEELSNEGDSQQSPGQQSPLPVEPEQGPAPLMVQLSLLRAETDRLREILAGKEREYQALVQRALQRLNEEARTYVLAPEPPTALSTDQGLVQWLQELNVDSGTIQMLLNHSFTLHTLLTYATRDDLIYTRIRGGMVCRIWRAILAQRAGSTPVTSGP

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Bovine
78
Sheep
78
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O95382 作为底物

Site PTM Type Enzyme
S251 Phosphorylation
S367 Phosphorylation
K540 Ubiquitination
K677 Ubiquitination
Y717 Phosphorylation
K773 Ubiquitination
K795 Ubiquitination
T801 Phosphorylation
T806 Phosphorylation O95382 (MAP3K6)
S896 Phosphorylation
T899 Phosphorylation
K910 Ubiquitination
S912 Phosphorylation
S917 Phosphorylation
S964 Phosphorylation
S984 Phosphorylation
K999 Ubiquitination
S1129 Phosphorylation
S1149 Phosphorylation
S1281 Phosphorylation

翻译修饰 - O95382 作为激酶

Substrate Site Source
O95382 (MAP3K6) T806 Uniprot
P45983 (MAPK8) T183 Uniprot
P45983 (MAPK8) Y185 Uniprot
Q16539 (MAPK14) T180 Uniprot
Q16539 (MAPK14) Y182 Uniprot
Q99683 (MAP3K5) T838 Uniprot

研究背景

功能:

Component of a protein kinase signal transduction cascade. Activates the JNK, but not ERK or p38 kinase pathways.

亚基结构:

Binds both upstream activators and downstream substrates in multimolecular complexes.

蛋白家族:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

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