(2)
(12)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0352, RRID:AB_2834197.
展开/折叠
EBBP; Estrogen responsive B box protein; Estrogen-responsive B box protein; TRI16_HUMAN; TRIM 16; TRIM16; Tripartite motif containing 16; Tripartite motif containing protein 16; Tripartite motif-containing protein 16;
抗原和靶标
Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.
- O95361 TRI16_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAELDLMAPGPLPRATAQPPAPLSPDSGSPSPDSGSASPVEEEDVGSSEKLGRETEEQDSDSAEQGDPAGEGKEVLCDFCLDDTRRVKAVKSCLTCMVNYCEEHLQPHQVNIKLQSHLLTEPVKDHNWRYCPAHHSPLSAFCCPDQQCICQDCCQEHSGHTIVSLDAARRDKEAELQCTQLDLERKLKLNENAISRLQANQKSVLVSVSEVKAVAEMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMAAISNTVQFLEEYCKFKNTEDITFPSVYVGLKDKLSGIRKVITESTVHLIQLLENYKKKLQEFSKEEEYDIRTQVSAVVQRKYWTSKPEPSTREQFLQYAYDITFDPDTAHKYLRLQEENRKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVEIFGAGTYVGLTCKGIDRKGEERNSCISGNNFSWSLQWNGKEFTAWYSDMETPLKAGPFRRLGVYIDFPGGILSFYGVEYDTMTLVHKFACKFSEPVYAAFWLSKKENAIRIVDLGEEPEKPAPSLVGTAP
翻译修饰 - O95361 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S24 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| T55 | Phosphorylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K188 | Methylation | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| K264 | Acetylation | Uniprot | |
| K266 | Acetylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| K354 | Ubiquitination | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| Y429 | Phosphorylation | Uniprot | |
| Y430 | Phosphorylation | Uniprot | |
| Y441 | Phosphorylation | Uniprot | |
| Y531 | Phosphorylation | Uniprot | |
| S537 | Phosphorylation | Uniprot | |
| K554 | Ubiquitination | Uniprot |
研究背景
E3 ubiquitin ligase that plays an essential role in the organization of autophagic response and ubiquitination upon lysosomal and phagosomal damages. Plays a role in the stress-induced biogenesis and degradation of protein aggresomes by regulating the p62-KEAP1-NRF2 signaling and particularly by modulating the ubiquitination levels and thus stability of NRF2. Acts as a scaffold protein and facilitates autophagic degradation of protein aggregates by interacting with p62/SQSTM, ATG16L1 and LC3B/MAP1LC3B. In turn, protects the cell against oxidative stress-induced cell death as a consequence of endomembrane damage.
Phosphorylated by ULK1.
Auto-ubiquitinates via its B-Boxes.
Cytoplasm.
Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.
Homodimerizes via its coiled-coil domain. Heterodimerizes with MID1, TRIM24 and PML. Interacts with Galectin-3/LGALS3 in a ULK1-dependent manner; this interaction mediates autophagy of damage endomembranes. Interacts with BECN1. Interacts with ATG16L1. Interacts with p62/SQSTM and LC3B/MAP1LC3B.
Belongs to the TRIM/RBCC family.
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