产品: TNFSF14 抗体
货号: AF0329
描述: Rabbit polyclonal antibody to TNFSF14
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Dog
分子量: 26kDa; 26kD(Calculated).
蛋白号: O43557
RRID: AB_2834173

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产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Dog(83%)
克隆:
Polyclonal
特异性:
TNFSF14 Antibody detects endogenous levels of total TNFSF14.
RRID:
AB_2834173
引用格式: Affinity Biosciences Cat# AF0329, RRID:AB_2834173.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD 258; CD258; CD258 antigen; Delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator A; Herpesvirus entry mediator ligand; herpesvirus entry mediator-ligand; HVEM L; HVEM-L; HVEML; Ligand for herpesvirus entry mediator; LIGHT; LTg; soluble form; TNF14; TNF14_HUMAN; TNFSF 14; Tnfsf14; TNFSF14 protein; TR 2; TR2; Tumor necrosis factor (ligand) superfamily, member 14; Tumor necrosis factor ligand superfamily member 14; Tumor necrosis factor ligand superfamily, member 14; Tumor necrosis factor receptor like 2; tumor necrosis factor receptor-like 2; Tumor necrosis factor superfamily member 14; Tumor necrosis factor superfamily member LIGHT;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
O43557 TNF14_HUMAN:

Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.

描述:
Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
序列:
MEESVVRPSVFVVDGQTDIPFTRLGRSHRRQSCSVARVGLGLLLLLMGAGLAVQGWFLLQLHWRLGEMVTRLPDGPAGSWEQLIQERRSHEVNPAAHLTGANSSLTGSGGPLLWETQLGLAFLRGLSYHDGALVVTKAGYYYIYSKVQLGGVGCPLGLASTITHGLYKRTPRYPEELELLVSQQSPCGRATSSSRVWWDSSFLGGVVHLEAGEKVVVRVLDERLVRLRDGTRSYFGAFMV

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Dog
83
Rabbit
70
Horse
60
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O43557 作为底物

Site PTM Type Enzyme
S32 Phosphorylation
S34 Phosphorylation
T161 Phosphorylation
T163 Phosphorylation
Y167 Phosphorylation
T170 Phosphorylation

研究背景

功能:

Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Acts as a ligand for TNFRSF14/HVEM. Upon binding to TNFRSF14/HVEM, delivers costimulatory signals to T cells, leading to T cell proliferation and IFNG production.

翻译修饰:

N-glycosylated.

The soluble form of isoform 1 derives from the membrane form by proteolytic processing.

细胞定位:

Cell membrane>Single-pass type II membrane protein.

Secreted.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.

亚基结构:

Homotrimer. Interacts with TNFRSF14.

蛋白家族:

Belongs to the tumor necrosis factor family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

文献引用

1). Hepatic stellate cell mediates transcription of TNFSF14 in hepatocellular carcinoma cells via H2S/CSE-JNK/JunB signaling pathway. Cell Death & Disease (PubMed: 35292636) [IF=9.0]

Application: WB    Species: Human    Sample: HCC cells

Fig. 3 H2S upregulates pro-apoptotic factor TNFSF14 expression in HCC cells. A The heatmap. B Venn diagram of gene predictions based on the control group and HepG2 cells treated with NaHS and co-cultured with LX-2 cells. C The expression levels of TNFSF14 mRNA changed in HepG2 cells treated with NaHS and co-cultured with LX-2 cells. D RT-qPCR and (E) Western blotting were performed to examine the effects of various concentrations of NaHS on expression of TNFSF14 gene in HepG2 and PLC/PRF/5 cells. F RT-qPCR and (G) Western blotting analysis of the expression of TNFSF14 under 10−3 M NaHS treatment with different time in HepG2 and PLC/PRF/5 cells. H RT-qPCR and (I) Western blotting were used to detect the effects of PPG treated on TNFSF14 expression in HCC cells. All Bars, ± SD; n = 3; *P < 0.05 compared with control in HepG2 cells, #P < 0.05 compared with control in PLC/PRF/5 cells, &P < 0.05 compared with the co-culture group in HCC cells.

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