Tubulin alpha Antibody - #AF0524

Figure 4. OTUB1 interacts with β-catenin and regulates its stability. A. Heatmap of RNA-seq expression data for EJ cell transfected with control or shOTUB1 lentivirus. B. GSEA of RNA-seq data revealed that OTUB1 target genes were involved in Wnt/β-catenin signaling pathway. C, D. Relative expression of β-catenin following overexpressed or knockdown OTUB1. E. Relative expression of β-catenin following overexpressed or knockdown OTUB1 (treated with or without MG132 for 8h). F. Relative expression of β-catenin following overexpressed or knockdown OTUB1 (treated with MG132 or chloroquine for 8h). G. Relative expression of β-catenin following overexpressed or knockdown OTUB1 (treated with or without CHX for 0h, 4h, 8h and 24h). H. Relative mRNA expression of OTUB1 and β-catenin following knockdown OTUB1. I. The interaction between OTUB1 and β-catenin was determined by a co-immunoprecipitation assay.

Fig. 2 Conditional knockout of PP2Cm in DRG neurons induces pain hypersensitivity a, Representative cartoons of pAAV9-hSyn-(Cre) injection. Intrathecal injection of pAAV9-hSyn-Cre or pAAV9-hSyn into male and female PP2Cmfl/fl mice resulted in the generation of PP2Cm-cKO and PP2Cm-ctrl mice. b, Representative immunoblots of PP2Cm in the L4-L5 DRGs on day 21 post-injection in PP2Cm-cKO and PP2Cm-ctrl mice of both sexes. male n = 3 experimental repeats (6 mice)/group, female n = 3 experimental repeats (6 mice)/group. c-e, Mechanical withdrawal thresholds (c), withdrawal latencies to heat (d) and cold stimulus (e) in PP2Cm-cKO and PP2Cm-ctrl mice. n = 10 mice/group, both sexes. f, BCAA concentrations in the L4-L5 DRGs of PP2Cm-cKO and PP2Cm-ctrl mice. n = 5 mice/group, both sexes. g-i, Mechanical withdrawal thresholds (g), withdrawal latencies to heat (h) and cold stimulus (i) in PP2Cm-cKO mice with or without BT2 supplementation after 7 consecutive days, PP2Cm-cKO + Vehicle vs. PP2Cm-cKO + BT2. n = 6 mice/group, both sexes. j, BCAA concentrations in the L4-L5 DRGs of PP2Cm-cKO + Vehicle and PP2Cm-cKO + BT2 mice. n = 6 mice/group, both sexes. Data are shown as the mean ± SEM. Statistical tests used were unpaired two-tailed Student’s t-test (c-e, g-i), one-way ANOVA with Tukey’s multiple comparisons test (b), and two-way repeated-measures ANOVA with Bonferroni’s post hoc test (f, j). *p