产品: SENP6 抗体
货号: AF0277
描述: Rabbit polyclonal antibody to SENP6
应用: WB IHC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 126kDa; 126kD(Calculated).
蛋白号: Q9GZR1
RRID: AB_2834163

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
SENP6 Antibody detects endogenous levels of total SENP6.
RRID:
AB_2834163
引用格式: Affinity Biosciences Cat# AF0277, RRID:AB_2834163.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2810017C20Rik; FLJ11355; FLJ11887; KIAA0389; KIAA0797; SENP 6; Senp6; SENP6_HUMAN; Sentrin specific protease 6; Sentrin-specific protease 6; Sentrin/SUMO specific protease SENP6; Sentrin/SUMO-specific protease SENP6; SSP 1; SSP1; SUMO 1 specific protease 1; SUMO 1 specific protease; SUMO-1-specific protease 1; SUMO1 specific protease 1; SUMO1 specific protease; SUMO1/sentrin specific peptidase 6; SUSP 1; SUSP1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9GZR1 SENP6_HUMAN:

Highly expressed in reproductive organs, such as testis, ovary and prostate.

描述:
SENP6 Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Does not seem to be involved in the processing of full-length SUMO proteins to their mature forms. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. May act preferentially on substrates containing 3 or more SUMO2 or SUMO3 moieties. Belongs to the peptidase C48 family.
序列:
MAAGKSGGSAGEITFLEALARSESKRDGGFKNNWSFDHEEESEGDTDKDGTNLLSVDEDEDSETSKGKKLNRRSEIVANSSGEFILKTYVRRNKSESFKTLKGNPIGLNMLSNNKKLSENTQNTSLCSGTVVHGRRFHHAHAQIPVVKTAAQSSLDRKERKEYPPHVQKVEINPVRLSRLQGVERIMKKTEESESQVEPEIKRKVQQKRHCSTYQPTPPLSPASKKCLTHLEDLQRNCRQAITLNESTGPLLRTSIHQNSGGQKSQNTGLTTKKFYGNNVEKVPIDIIVNCDDSKHTYLQTNGKVILPGAKIPKITNLKERKTSLSDLNDPIILSSDDDDDNDRTNRRESISPQPADSACSSPAPSTGKVEAALNENTCRAERELRSIPEDSELNTVTLPRKARMKDQFGNSIINTPLKRRKVFSQEPPDALALSCQSSFDSVILNCRSIRVGTLFRLLIEPVIFCLDFIKIQLDEPDHDPVEIILNTSDLTKCEWCNVRKLPVVFLQAIPAVYQKLSIQLQMNKEDKVWNDCKGVNKLTNLEEQYIILIFQNGLDPPANMVFESIINEIGIKNNISNFFAKIPFEEANGRLVACTRTYEESIKGSCGQKENKIKTVSFESKIQLRSKQEFQFFDEEEETGENHTIFIGPVEKLIVYPPPPAKGGISVTNEDLHCLNEGEFLNDVIIDFYLKYLVLEKLKKEDADRIHIFSSFFYKRLNQRERRNHETTNLSIQQKRHGRVKTWTRHVDIFEKDFIFVPLNEAAHWFLAVVCFPGLEKPKYEPNPHYHENAVIQKCSTVEDSCISSSASEMESCSQNSSAKPVIKKMLNKKHCIAVIDSNPGQEESDPRYKRNICSVKYSVKKINHTASENEEFNKGESTSQKVADRTKSENGLQNESLSSTHHTDGLSKIRLNYSDESPEAGKMLEDELVDFSEDQDNQDDSSDDGFLADDNCSSEIGQWHLKPTICKQPCILLMDSLRGPSRSNVVKILREYLEVEWEVKKGSKRSFSKDVMKGSNPKVPQQNNFSDCGVYVLQYVESFFENPILSFELPMNLANWFPPPRMRTKREEIRNIILKLQEDQSKEKRKHKDTYSTEAPLGEGTEQYVNSISD

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9GZR1 作为底物

Site PTM Type Enzyme
S6 Phosphorylation
S9 Phosphorylation
S22 Phosphorylation
S35 Phosphorylation
S42 Phosphorylation
S55 Phosphorylation
K99 Methylation
T100 Phosphorylation
K102 Methylation
K189 Sumoylation
Y214 Phosphorylation
T217 Phosphorylation
S221 Phosphorylation
S224 Phosphorylation
K226 Ubiquitination
K264 Ubiquitination
K274 Sumoylation
K319 Ubiquitination
T323 Phosphorylation
S335 Phosphorylation
S336 Phosphorylation
T345 Phosphorylation
S350 Phosphorylation
S352 Phosphorylation
S361 Phosphorylation
S362 Phosphorylation
T398 Phosphorylation
K406 Sumoylation
S412 Phosphorylation
T416 Phosphorylation
K419 Sumoylation
K534 Ubiquitination
S577 Phosphorylation
Y599 Phosphorylation
S606 Phosphorylation
K736 Ubiquitination
S819 Phosphorylation
S869 Phosphorylation
K910 Ubiquitination
S916 Phosphorylation
S919 Phosphorylation
S985 Phosphorylation
K1086 Ubiquitination
S1111 Phosphorylation

研究背景

功能:

Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in reproductive organs, such as testis, ovary and prostate.

亚基结构:

Interacts with RXRA. Forms a complex with KAT5-TIP60 and UBE2I in response to UV irradiation. Interacts with RPA1 to maintain it in hyposumoylated state during S phase preventing DNA repair initiation.

蛋白家族:

Belongs to the peptidase C48 family.

文献引用

1). SENP6-Mediated deSUMOylation of VEGFR2 Enhances Its Cell Membrane Transport in Angiogenesis. International Journal of Molecular Sciences (PubMed: 36768878) [IF=5.6]

Application: WB    Species: Human    Sample: HUVECs

Figure 3 SENP6−reduced HUVECs attenuate AGE−induced angiogenic signaling. (A) Heat map of mRNA showing the differences in SENPs expression between the control and AGEs groups. “Red” indicates up-regulation and “Green” indicates doregulation. ** indicates that p < 0.01 and *** indicates that p < 0.001 between the indicated groups. (B) Detection of SENP6 mRNA expression by qPCR at 0, 4, 8, and 12 h of AGE−stimulated HUVECs, n = 4, * p < 0.05 vs. 0 h. (C) The temporal effect of AGE stimulation on HUVECs was investigated using Western blot, which showed SENP6 protein expression at 0, 4, 8, and 12 h. (D) Analysis of protein expression level results, n = 6, * p < 0.05 vs. 0 h. AGE−induced angiogenic responses were inhibited by SENP6 silencing (Supplemental Figure S1C). HUVECs were transfected with NC siRNA or SENP6 siRNA for 48 h. Cells were subjected to HUVECs migration and tube formation in response to AGEs. (E) Transwell migration assay for the migration ability. (F) Analysis of migration assay results, n = 3, * p < 0.05 vs. NC siRNA, # p < 0.05 vs. NC siRNA + AGEs, Scale bar: 100 μm. (G) HUVECs tube formation in a Matrigel assay. Representative images are shown in (G). (H,I) Analysis of total tube length and total branching points results, n = 4, * p < 0.05 vs. NC siRNA, # p < 0.05 vs. NC siRNA + AGEs, Scale bar: 100 μm (J) HUVECs migration by a scratch assay for indicated times. (K) Wound healing (% closure) was quantified, n = 5, * p < 0.05 vs. NC siRNA, # p < 0.05 vs. NC siRNA + AGEs, Scale bar: 100 μm. * and # indicate significance between the indicated groups.

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