产品: SKP2/p45 抗体
货号: AF0505
描述: Rabbit polyclonal antibody to SKP2/p45
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 47kDa; 48kD(Calculated).
蛋白号: Q13309
RRID: AB_2834158

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(100%), Dog(90%), Chicken(80%)
克隆:
Polyclonal
特异性:
SKP2/p45 Antibody detects endogenous levels of total SKP2/p45.
RRID:
AB_2834158
引用格式: Affinity Biosciences Cat# AF0505, RRID:AB_2834158.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CDK2/Cyclin A associated protein p45; Cyclin A/CDK2 associated protein p45; Cyclin-A/CDK2-associated protein p45; F box protein Skp2; F box/LRR repeat protein 1; F-box protein Skp2; F-box/LRR-repeat protein 1; FBL 1; FBL1; FBXL 1; FBXL1; FLB 1; FLB1; MGC1366; p45; p45skp2; S phase kinase associated protein 2 (p45); S phase kinase associated protein 2; S-phase kinase-associated protein 2; S-phase kinase-associated protein 2 E3 ubiquitin protein ligase; SKP 2; Skp2; SKP2_HUMAN;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
SKP2 an F-box protein of the SCF (SKP1-CUL1-F- box protein) E3 ubiquitin ligase system. The F-box component of the SCF complex is responsible for recognizing different substrates for ubiquitination. The SCF E3 complex mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Three alternatively-spliced isoforms have been described.
序列:
MHRKHLQEIPDLSSNVATSFTWGWDSSKTSELLSGMGVSALEKEEPDSENIPQELLSNLGHPESPPRKRLKSKGSDKDFVIVRRPKLNRENFPGVSWDSLPDELLLGIFSCLCLPELLKVSGVCKRWYRLASDESLWQTLDLTGKNLHPDVTGRLLSQGVIAFRCPRSFMDQPLAEHFSPFRVQHMDLSNSVIEVSTLHGILSQCSKLQNLSLEGLRLSDPIVNTLAKNSNLVRLNLSGCSGFSEFALQTLLSSCSRLDELNLSWCFDFTEKHVQVAVAHVSETITQLNLSGYRKNLQKSDLSTLVRRCPNLVHLDLSDSVMLKNDCFQEFFQLNYLQHLSLSRCYDIIPETLLELGEIPTLKTLQVFGIVPDGTLQLLKEALPHLQINCSHFTTIARPTIGNKKNQEIWGIKCRLTLQKPSCL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Rabbit
100
Horse
90
Dog
90
Chicken
80
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13309 作为底物

Site PTM Type Enzyme
K43 Sumoylation
S48 Phosphorylation
S57 Phosphorylation
S64 Phosphorylation P11309 (PIM1) , P24941 (CDK2)
K68 Acetylation
K71 Acetylation
S72 Phosphorylation P31749 (AKT1) , P11309 (PIM1)
K73 Ubiquitination
S75 Phosphorylation
K77 Ubiquitination
K125 Ubiquitination
K145 Ubiquitination
S179 Phosphorylation
S212 Phosphorylation
K228 Ubiquitination
K299 Ubiquitination
S303 Phosphorylation
K405 Ubiquitination
K413 Ubiquitination
T417 Phosphorylation P11309 (PIM1)
K420 Ubiquitination

研究背景

功能:

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.

Through the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, has an antiviral activity towards that virus.

翻译修饰:

Ubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Deubiquitinated by USP13.

Acetylation at Lys-68 and Lys-71 increases stability through impairment of APC/C-mediated proteolysis and promotes cytoplasmic retention. Deacetylated by SIRT3.

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Part of a SCF(SKP2) complex consisting of CUL1, RBX1, SKP1 and SKP2. Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Interacts directly with CUL1 and SKP1. Interacts with CKS1. Interacts with the cyclin-A-CDK2 complex. Interacts with ORC1, phosphorylated CDT1, phosphorylated RBL2, ELF4, phosphorylated RAG2, FOXO1, UBP43, MYC, TOB1, TAL1 and KMT2A/MLL1. Interacts with TRIM21. Interacts with IFI27; promotes the ubiquitin-mediated proteasomal degradation of NS5A. Interacts with hepatitis C virus/HCV non-structural protein NS5A; promotes the ubiquitin-mediated proteasomal degradation of NS5A.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

文献引用

1). Wang X et al. Bexarotene improves motor function after spinal cord injury in mice. Neural Regeneration Research 2023 Dec;18(2733-2742) (PubMed: 37449638) [IF=6.1]

Application: WB    Species: Mouse    Sample: spinal cord

Figure 10 Bexarotene activates TFE3 through the AMPK-mTOR and AMPK-SPK2- CARM1 signaling pathways in SCI. (A) Western blot assay of the cytoplasmic expression levels of AMPK, p-AMPK, mTOR, and p-mTOR. (B) Quantification of AMPK, p-AMPK, mTOR, and p-mTOR from A, normalized to GADPH. (C) Western blot assay of the nuclear expression levels of AMPK, p-AMPK, FOXO3a, p-FOXO3a, SKP2, and CARM1. (D) Quantification of AMPK, p-AMPK, FOXO3a, p-FOXO3a, SKP2, and CARM1 from C, normalized to H3. (E, F) Immunoprecipitation images showing nuclear CARM1–TFE3 binding. Data are expressed as the mean ± SEM (n = 6 per group). **P < 0.01, vs. SCI group; ##P < 0.01, vs. SCI + Bex group. One-way analysis of variance with least significance difference post hoc test. (p-)AMPK: (phospho-) adenosine 5′-monophosphate-activated protein kinase; (p-)FOXO3a: (phospho-)forkhead box O3; (p-)mTOR: (phospho-)mammalian target of rapamycin; Bex: bexarotene; CARM1: coactivator-associated arginine methyltransferase 1; CC: compound C; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; H3: histone protein H3; SCI: spinal cord injury; SKP2: S-phase kinase-associated protein 2; TFE3: transcription factor E3.

2). Wang X et al. 贝沙罗汀改善脊髓损伤后运动功能的机制. 中国神经再生研究(英文版) 2023 Dec;18(2733-2742) (PubMed: 37449638) [IF=6.1]

Application: WB    Species: Mouse    Sample:

Figure 10 Bexarotene activates TFE3 through the AMPK-mTOR and AMPK-SPK2- CARM1 signaling pathways in SCI. (A) Western blot assay of the cytoplasmic expression levels of AMPK, p-AMPK, mTOR, and p-mTOR. (B) Quantification of AMPK, p-AMPK, mTOR, and p-mTOR from A, normalized to GADPH. (C) Western blot assay of the nuclear expression levels of AMPK, p-AMPK, FOXO3a, p-FOXO3a, SKP2, and CARM1. (D) Quantification of AMPK, p-AMPK, FOXO3a, p-FOXO3a, SKP2, and CARM1 from C, normalized to H3. (E, F) Immunoprecipitation images showing nuclear CARM1–TFE3 binding. Data are expressed as the mean ± SEM (n = 6 per group). **P < 0.01, vs. SCI group; ##P < 0.01, vs. SCI + Bex group. One-way analysis of variance with least significance difference post hoc test. (p-)AMPK: (phospho-) adenosine 5′-monophosphate-activated protein kinase; (p-)FOXO3a: (phospho-)forkhead box O3; (p-)mTOR: (phospho-)mammalian target of rapamycin; Bex: bexarotene; CARM1: coactivator-associated arginine methyltransferase 1; CC: compound C; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; H3: histone protein H3; SCI: spinal cord injury; SKP2: S-phase kinase-associated protein 2; TFE3: transcription factor E3.

3). Hu R et al. Calycosin inhibited autophagy and oxidative stress in chronic kidney disease skeletal muscle atrophy by regulating AMPK/SKP2/CARM1 signalling pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 2020 Oct;24(19):11084-11099. (PubMed: 32910538) [IF=5.3]

Application: WB    Species: rat    Sample: muscles

FIGURE 5|Effect of calycosin on the expression of CARM1 and H3R17me2a,and related proteins in rat muscles. A-B,Representative images and scores of the expression of CARM1 and H3R17me2a in muscle sections using IHC staining (×200).Bar = 50 µm. *P < 0.05 compared to the sham group. ##P < 0.01 compared to the 5/6 Nx model group. C-E, Representative immune blots of key proteins involved in muscle atrophy and proteins associated with the AMPK/SKP2/CARM1 signalling pathway

4). Zhang Q et al. S-phase kinase-associated protein 2 impairs the inhibitory effects of miR-1236-3p on bladder tumors. American Journal of Translational Research 2018 Mar 15;10(3):731-743 (PubMed: 29636863) [IF=2.2]

Application: WB    Species: human    Sample: 5637, T24, EJ and J82 cells and SV-HUC-1

Figure 1. |Skp2 is over-expressed in BCa samples and cell lines.(E) Skp2 mRNA and protein (G) expression was increased in 5637, T24, EJ and J82 cell lines compared with primary normal human bladder epithelial cells (SV-HUC-1), (*P<0.05, **P<0.01). GAPDH levels were used as an internal control.

5). Yan Lv et al. WISP2/CCN5 gene knockdown in vitro and in vivo exhibits proliferation promotion of breast cancer through targeting Skp2 and p27Kip1. bioRxiv 2020 Jan 29

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