NCK2 Antibody - #AF0679
| 产品: | NCK2 抗体 |
| 货号: | AF0679 |
| 描述: | Rabbit polyclonal antibody to NCK2 |
| 应用: | WB IHC |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Rabbit, Dog, Chicken |
| 分子量: | 42kDa; 43kD(Calculated). |
| 蛋白号: | O43639 |
| RRID: | AB_2834155 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0679, RRID:AB_2834155.
展开/折叠
Cytoplasmic protein NCK2; GRB4; Growth factor receptor bound protein 4; NCK adaptor protein 2; Nck-2; NCK2; NCK2_HUMAN; NCKbeta; Non-catalytic region of tyrosine kinase adaptor protein 2; Noncatalytic region of tyrosine kinase beta; SH2/SH3 adaptor protein NCK-beta;
抗原和靶标
- O43639 NCK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O43639 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Acetylation | Uniprot | |
| T37 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | Uniprot | |
| Y50 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| T74 | Phosphorylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| T92 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| Y99 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| Y110 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| Y271 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| Y342 | Phosphorylation | Uniprot |
研究背景
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
Phosphorylated.
Cytoplasm. Endoplasmic reticulum.
Ubiquitous.
Interacts with DOCK1, LIMS1 and TGFB1I1. Part of a complex containing PPP1R15B, PP1 and NCK2. Interacts with FASLG (By similarity). Interacts with AXL. Interacts with PAK1, PKN2 and SOS1. Interacts (via SH2 domain) with EGFR. Interacts (via SH2 domain) with DDR1.
研究领域
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
限制条款
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