产品: HSP105 抗体
货号: AF0617
描述: Rabbit polyclonal antibody to HSP105
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 100kDa; 97kD(Calculated).
蛋白号: Q92598
RRID: AB_2834147

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(83%)
克隆:
Polyclonal
特异性:
HSP105 Antibody detects endogenous levels of total HSP105.
RRID:
AB_2834147
引用格式: Affinity Biosciences Cat# AF0617, RRID:AB_2834147.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Antigen NY CO 25; Antigen NY-CO-25; DKFZp686M05240; Heat shock 105kD alpha; Heat shock 105kD; Heat shock 105kD beta; Heat shock 105kDa protein 1; Heat shock 105kDa protein; Heat shock 105kDa/110kDa protein 1; Heat shock 110 kDa protein; Heat shock 110kDa protein; Heat shock protein 105 kDa; HS105_HUMAN; HSP105; HSP105A; HSP105B; HSP110; HSPH 1; Hsph1; KIAA0201; NY CO 25;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q92598 HS105_HUMAN:

Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.

描述:
HSP105 a molecular chaperone of the heat shock protein 70 family. Has ATPase activity. Expressed at especially high levels in mammalian brain and has been shown to suppress apoptosis in neuronal cells and to prevent the aggregation of proteins following heat shock. Known to interact with a variety of proteins including alpha-tubulin and the androgen receptor.
序列:
MSVVGLDVGSQSCYIAVARAGGIETIANEFSDRCTPSVISFGSKNRTIGVAAKNQQITHANNTVSNFKRFHGRAFNDPFIQKEKENLSYDLVPLKNGGVGIKVMYMGEEHLFSVEQITAMLLTKLKETAENSLKKPVTDCVISVPSFFTDAERRSVLDAAQIVGLNCLRLMNDMTAVALNYGIYKQDLPSLDEKPRIVVFVDMGHSAFQVSACAFNKGKLKVLGTAFDPFLGGKNFDEKLVEHFCAEFKTKYKLDAKSKIRALLRLYQECEKLKKLMSSNSTDLPLNIECFMNDKDVSGKMNRSQFEELCAELLQKIEVPLYSLLEQTHLKVEDVSAVEIVGGATRIPAVKERIAKFFGKDISTTLNADEAVARGCALQCAILSPAFKVREFSVTDAVPFPISLIWNHDSEDTEGVHEVFSRNHAAPFSKVLTFLRRGPFELEAFYSDPQGVPYPEAKIGRFVVQNVSAQKDGEKSRVKVKVRVNTHGIFTISTASMVEKVPTEENEMSSEADMECLNQRPPENPDTDKNVQQDNSEAGTQPQVQTDAQQTSQSPPSPELTSEENKIPDADKANEKKVDQPPEAKKPKIKVVNVELPIEANLVWQLGKDLLNMYIETEGKMIMQDKLEKERNDAKNAVEEYVYEFRDKLCGPYEKFICEQDHQNFLRLLTETEDWLYEEGEDQAKQAYVDKLEELMKIGTPVKVRFQEAEERPKMFEELGQRLQHYAKIAADFRNKDEKYNHIDESEMKKVEKSVNEVMEWMNNVMNAQAKKSLDQDPVVRAQEIKTKIKELNNTCEPVVTQPKPKIESPKLERTPNGPNIDKKEEDLEDKNNFGAEPPHQNGECYPNEKNSVNMDLD

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
83
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q92598 作为底物

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
T25 Phosphorylation
S31 Phosphorylation
R33 Methylation
S43 Phosphorylation
K44 Ubiquitination
K53 Ubiquitination
K68 Acetylation
K68 Methylation
K68 Ubiquitination
K82 Ubiquitination
K84 Ubiquitination
Y89 Phosphorylation
K95 Ubiquitination
K102 Ubiquitination
K126 Ubiquitination
S132 Phosphorylation
T138 Phosphorylation
T149 Phosphorylation
K185 Ubiquitination
K194 Acetylation
K194 Ubiquitination
K221 Ubiquitination
K234 Acetylation
K234 Ubiquitination
K239 Ubiquitination
K249 Acetylation
K249 Ubiquitination
R265 Methylation
K272 Acetylation
K272 Ubiquitination
K274 Acetylation
K275 Ubiquitination
K295 Ubiquitination
K300 Ubiquitination
S304 Phosphorylation
Y322 Phosphorylation
K351 Ubiquitination
K356 Ubiquitination
K360 Acetylation
K360 Ubiquitination
T365 Phosphorylation
S384 Phosphorylation
K388 Methylation
K388 Ubiquitination
S429 Phosphorylation
K430 Acetylation
K430 Ubiquitination
T433 Phosphorylation
Y446 Phosphorylation
K458 Ubiquitination
S468 Phosphorylation
K471 Ubiquitination
K475 Ubiquitination
T486 Phosphorylation
S493 Phosphorylation
T494 Phosphorylation
T503 Phosphorylation
S554 Phosphorylation
S557 Phosphorylation
T561 Phosphorylation
S562 Phosphorylation
K588 Ubiquitination
K590 Ubiquitination
Y614 Phosphorylation
K626 Ubiquitination
K635 Ubiquitination
Y641 Phosphorylation
Y643 Phosphorylation
K648 Ubiquitination
K655 Ubiquitination
T670 Phosphorylation
T672 Phosphorylation
Y677 Phosphorylation
Y688 Phosphorylation
K691 Ubiquitination
K697 Ubiquitination
T700 Phosphorylation
K703 Ubiquitination
K714 Ubiquitination
K749 Ubiquitination
K772 Ubiquitination
K790 Ubiquitination
K804 Ubiquitination
K806 Ubiquitination
S809 Phosphorylation
K811 Acetylation
K811 Ubiquitination
T815 Phosphorylation
K823 Ubiquitination
K824 Ubiquitination
S852 Phosphorylation

研究背景

功能:

Acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release. Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (By similarity).

翻译修饰:

Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.

亚基结构:

Interacts with HSPA8/HSC70 (By similarity). Interacts with HSPA1A (via NBD) and HSPA1B (via NBD).

蛋白家族:

Belongs to the heat shock protein 70 family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

文献引用

1). Knockdown of HSP110 attenuates hypoxia-induced pulmonary hypertension in mice through suppression of YAP/TAZ-TEAD4 pathway. RESPIRATORY RESEARCH (PubMed: 35986277) [IF=5.8]

Application: IF/ICC    Species: Mouse    Sample: lung tissues

Fig. 1HSP110 is upregulated in lung tissues of the hypoxia-induced PH in mice. a The RVSP of the normoxia and hypoxia groups in mice (N = 8). b H&E staining of pulmonary arteries in lung tissues in normoxia and hypoxia group (N = 8). Scale bars, 50 μm. c Quantification of ratio of pulmonary arterial medial thickness to total vessel size (media/CSA) (N = 8). Relative mRNA level (d) and protein level (e, f) of HSP110 in pulmonary arteries (N = 8). g Double immunofluorescence staining of YAP (green) and HSP110 (red) in pulmonary arteries (N = 8). Scale bars, 50 μm. White arrows pointed to HSP110 and YAP double-positive cells. h Double immunofluorescence staining of α-SMA (green) and HSP110 (red) in pulmonary arteries (N = 8). Scale bars, 50 μm. White arrows pointed to HSP110 and α-SMA double-positive cells. Data are means ± SD from 8 mice per group. *p 

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