产品: MMP7 抗体
货号: AF0218
描述: Rabbit polyclonal antibody to MMP7
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Sheep, Rabbit
分子量: 29kDa; 30kD(Calculated).
蛋白号: P09237
RRID: AB_2833348

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 50ul RMB¥ 1250 现货
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 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(94%), Sheep(83%), Rabbit(83%)
克隆:
Polyclonal
特异性:
MMP7 Antibody detects endogenous levels of total MMP7.
RRID:
AB_2833348
引用格式: Affinity Biosciences Cat# AF0218, RRID:AB_2833348.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Matrilysin; Matrin; Matrix Metalloproteinase 7; Matrix metalloproteinase-7; MMP 7; MMP-7; MMP7; MMP7_HUMAN; MPSL1; PUMP 1; Pump 1 protease; Pump-1 protease; PUMP1; Uterine matrilysin; Uterine metalloproteinase;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
MMP7 Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. Belongs to the peptidase M10A family. Note: This description may include information from UniProtKB.
序列:
MRLTVLCAVCLLPGSLALPLPQEAGGMSELQWEQAQDYLKRFYLYDSETKNANSLEAKLKEMQKFFGLPITGMLNSRVIEIMQKPRCGVPDVAEYSLFPNSPKWTSKVVTYRIVSYTRDLPHITVDRLVSKALNMWGKEIPLHFRKVVWGTADIMIGFARGAHGDSYPFDGPGNTLAHAFAPGTGLGGDAHFDEDERWTDGSSLGINFLYAATHELGHSLGMGHSSDPNAVMYPTYGNGDPQNFKLSQDDIKGIQKLYGKRSNSRKK

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
94
Sheep
83
Rabbit
83
Bovine
78
Dog
75
Chicken
71
Xenopus
59
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P09237 作为底物

Site PTM Type Enzyme
S47 Phosphorylation
T49 Phosphorylation
K84 Acetylation

研究背景

功能:

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.

细胞定位:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

研究领域

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

文献引用

1). Xu et al. SETD3 is regulated by a couple of microRNAs and plays opposing roles in proliferation and metastasis of hepatocellular carcinoma. Clinical Science 2019 Oct 30;133(20):2085-2105 (PubMed: 31654063) [IF=6.0]

2). Yin Y et al. MMP‐7 affects peritoneal ultrafiltration associated with elevated aquaporin‐1 expression via MAPK/ERK pathway in peritoneal mesothelial cells. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 2021 Jun 11. (PubMed: 34117704) [IF=5.3]

Application: WB    Species: Human    Sample: peritoneal mesothelial cells

FIGURE 2 High glucose induced the expression of MMP‐7 in peritoneal mesothelial cells. (a, b) Peritoneal mesothelial cells were seeded in the 6‐well plate (1 × 106 cells/well) and then stimulated with different concentrations of glucose for 24 hours. The mRNA level of MMP‐7 was detected through quantitative RT‐PCR (a), the protein level of MMP‐7 in the cells was detected by Western blotting analysis (b), and the production of MMP‐7 in the culture medium was detected by ELISA (c). (d‐e) Cells were incubated in dialysate with 4.25% glucose for indicated time points. The mRNA level of MMP‐7 was detected through quantitative RT‐PCR (d), the protein level of MMP‐7 in the cells was detected by Western blotting analysis (e), and the production of MMP‐7 in the culture medium was detected by ELISA (F). *P < 0.05, **P < 0.01, one of the three independent experiments is shown

Application: IF/ICC    Species: Human    Sample: peritoneal mesothelial cells

FIGURE 3 Recombinant MMP‐7 protein up‐regulated cell volume and enhanced aquaporin‐1 expression in peritoneal mesothelial cells. The HMrSV5 cells were seeded in the 6‐well plate (1 × 106 cells/well). (a) Cells were stimulated with MMP‐7 protein for 12 hours, followed by incubating in normal saline (NS) or dialysate with 4.25% glucose for 1 minute. The cell diameter of peritoneal mesothelial cells was evaluated by the Scepter 2.0 cell counter. The changes of cell diameter after glucose stimulation, with or without MMP‐7 incubation, were calculated. (b, c) The cells were treated with 50 ng/ml MMP‐7 for different time points. The mRNA level of AQP‐1 was detected by quantitative RT‐PCR (b), and the protein levels of AQP‐1 were detected by Western blotting (c). (d, e) The cells were treated with different concentrations of MMP‐7 for different time points. The mRNA level of AQP‐1 was detected by quantitative RT‐PCR (d), and the protein levels of AQP‐1 were detected by Western blotting (e). (f) The cells were treated with indicated 50 ng/ml MMP‐7 for 12 hours. The cells were then permeabilized by Triton‐X 100, and immunofluorescence assay was used to detect the expression of AQP‐1 (red, with DAPI‐stained blue nuclei). (g, h) The cells were treated with the indicated 50 ng/ml MMP‐7 for 12 hours. The expression of AQP‐1 in the HMrSV5 cell membrane was evaluated by Western blotting (g) and immunofluorescence assay (H). *P < 0.05, **P < 0.01, one of the three independent experiments is shown

3). Feng T et al. The microRNA‑708‑5p/ZEB1/EMT axis mediates the metastatic potential of osteosarcoma. ONCOLOGY REPORTS 2020 Feb;43(2):491-502 (PubMed: 31894343) [IF=4.2]

Application: WB    Species: Human    Sample: OS cells

Figure 3. miR-708-5p suppresses epithelial-to-mesenchymal transition (EMT) of OS cells. (A) mRNA and (C, left column) protein levels of MMP2, MMP7, MMP9, N-cadherin, vimentin, E-cadherin and Snail after miR-708-5p overexpression (708-5p mimics) compared to the scramble negative control (NC mimic) in MG63 cells. (B) mRNA and (C, right column) protein levels of MMP2, MMP9, N-cadherin, vimentin, E-cadherin and Snail after miR-708-5p overexpression (708-5p mimics) compared to the scramble negative control (NC mimic) in SaOS-2 cells. Relative protein expression levels in (D) MG63 and (E) SaOS-2 cells. All data are presented as mean ± SD from at least three independent experiments. *P<0.05, **P<0.01, NC mimic vs. 708-5p mimic. OS, osteosarcoma; MMP, matrix metalloproteinase.

4). Dongdong Wang et al. Auxiliary antitumor effects of fungal proteins from Hericium erinaceus by target on the gut microbiota. Journal of Food Science 2020 Jun;85(6):1872-1890. (PubMed: 32460371) [IF=3.9]

Application: WB    Species: Mice    Sample: tumor tissues

Figure 12–Effects of fungal proteins extracted from Hericium erinaceus on tumor tissues in xenografted cancer mice. (A-H) is the QT-PCR analysis results of some key genes in the tumor tissues; (I) is the western bolting analysis results of some key proteins in the tumor tissues; (J) is the level of LPS in serum; (K) is the plasma concentration of 5-Fu. Animals were randomly divided into nine groups: control (no treatments), xenograft-only (Model), HEP-only (FP), pre-xenograft HEP (PN), pre-xenograft HEP+5-Fu (PF), HEP+5-Fu (NP), 5-Fu-only (NF), antibiotics+HEP+ 5-Fu (AP), and antibiotics+5-Fu (AF). Only the control and HEP-only groups had no xenografts. Values are expressed as means ± SDs (n = 8), #P < 0.05 compared with control group, ∗P < 0.05, ∗∗P < 0.01 compared with model group, indicates significant differences compared to the model group.

5). Zhang Z et al. Downregulation of LncRNA Gas5 Inhibits Apoptosis and Inflammation after Spinal Cord Ischemia-Reperfusion in Rats. BRAIN RESEARCH BULLETIN 2020 Dec 11;S0361-9230(20)30714-0. (PubMed: 33316370) [IF=3.8]

6). Wang J et al. KRT17 Accelerates Cell Proliferative and Invasive Potential of Laryngeal Squamous Cell Carcinoma (LSCC) through Regulating AKT/mTOR and Wnt/β-Catenin Pathways. Evidence-based Complementary and Alternative Medicine 2022 Oct 5;2022:6176043. (PubMed: 36248412)

Application: WB    Species: Human    Sample: TU177 cells

Figure 5 KRT17 regulates LSCC progression by affecting AKT/mTOR and Wnt/β-catenin axes (a). The protein-protein interaction (PPI) network of the KRT17 from STRING online database (https://string-db.org) was constructed (b). The protein levels in AMC-HN-8 cells were evaluated using western blot (c). The protein levels in TU177 cells were estimated using western blot. ∗A significant difference compared with the sh-NC group. ∗∗P < 0.01 and ∗∗∗P < 0.001.

7). Dongdong et al. Fungal Proteins from Hericium Erinaceus Show Auxiliary Antitumor Effects with 5-Fluoro-2,4(1H,3H)-Pyrimidinedione by Improving the Gut Microbiota in Mice.

8). Sun C et al. Hsa-miR-326 targets CCND1 and inhibits non-small cell lung cancer development. Oncotarget 2016 Feb 16;7(7):8341-59 (PubMed: 26840018)

Application: WB    Species:    Sample:

Figure 7: Ectopic expression of miR-326 in A549 and SPC-A-1 cells reduces cell migration and invasion motility. (E) Expression of MMP-7 and MMP-9 protein in A549 and SPC-A-1 cells after transfection.

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