MMP7 Antibody - #AF0218

FIGURE 2 High glucose induced the expression of MMP‐7 in peritoneal mesothelial cells. (a, b) Peritoneal mesothelial cells were seeded in the 6‐well plate (1 × 106 cells/well) and then stimulated with different concentrations of glucose for 24 hours. The mRNA level of MMP‐7 was detected through quantitative RT‐PCR (a), the protein level of MMP‐7 in the cells was detected by Western blotting analysis (b), and the production of MMP‐7 in the culture medium was detected by ELISA (c). (d‐e) Cells were incubated in dialysate with 4.25% glucose for indicated time points. The mRNA level of MMP‐7 was detected through quantitative RT‐PCR (d), the protein level of MMP‐7 in the cells was detected by Western blotting analysis (e), and the production of MMP‐7 in the culture medium was detected by ELISA (F). *P < 0.05, **P < 0.01, one of the three independent experiments is shown

FIGURE 3 Recombinant MMP‐7 protein up‐regulated cell volume and enhanced aquaporin‐1 expression in peritoneal mesothelial cells. The HMrSV5 cells were seeded in the 6‐well plate (1 × 106 cells/well). (a) Cells were stimulated with MMP‐7 protein for 12 hours, followed by incubating in normal saline (NS) or dialysate with 4.25% glucose for 1 minute. The cell diameter of peritoneal mesothelial cells was evaluated by the Scepter 2.0 cell counter. The changes of cell diameter after glucose stimulation, with or without MMP‐7 incubation, were calculated. (b, c) The cells were treated with 50 ng/ml MMP‐7 for different time points. The mRNA level of AQP‐1 was detected by quantitative RT‐PCR (b), and the protein levels of AQP‐1 were detected by Western blotting (c). (d, e) The cells were treated with different concentrations of MMP‐7 for different time points. The mRNA level of AQP‐1 was detected by quantitative RT‐PCR (d), and the protein levels of AQP‐1 were detected by Western blotting (e). (f) The cells were treated with indicated 50 ng/ml MMP‐7 for 12 hours. The cells were then permeabilized by Triton‐X 100, and immunofluorescence assay was used to detect the expression of AQP‐1 (red, with DAPI‐stained blue nuclei). (g, h) The cells were treated with the indicated 50 ng/ml MMP‐7 for 12 hours. The expression of AQP‐1 in the HMrSV5 cell membrane was evaluated by Western blotting (g) and immunofluorescence assay (H). *P < 0.05, **P < 0.01, one of the three independent experiments is shown

Figure 3. miR-708-5p suppresses epithelial-to-mesenchymal transition (EMT) of OS cells. (A) mRNA and (C, left column) protein levels of MMP2, MMP7, MMP9, N-cadherin, vimentin, E-cadherin and Snail after miR-708-5p overexpression (708-5p mimics) compared to the scramble negative control (NC mimic) in MG63 cells. (B) mRNA and (C, right column) protein levels of MMP2, MMP9, N-cadherin, vimentin, E-cadherin and Snail after miR-708-5p overexpression (708-5p mimics) compared to the scramble negative control (NC mimic) in SaOS-2 cells. Relative protein expression levels in (D) MG63 and (E) SaOS-2 cells. All data are presented as mean ± SD from at least three independent experiments. *P<0.05, **P<0.01, NC mimic vs. 708-5p mimic. OS, osteosarcoma; MMP, matrix metalloproteinase.

Figure 12–Effects of fungal proteins extracted from Hericium erinaceus on tumor tissues in xenografted cancer mice. (A-H) is the QT-PCR analysis results of some key genes in the tumor tissues; (I) is the western bolting analysis results of some key proteins in the tumor tissues; (J) is the level of LPS in serum; (K) is the plasma concentration of 5-Fu. Animals were randomly divided into nine groups: control (no treatments), xenograft-only (Model), HEP-only (FP), pre-xenograft HEP (PN), pre-xenograft HEP+5-Fu (PF), HEP+5-Fu (NP), 5-Fu-only (NF), antibiotics+HEP+ 5-Fu (AP), and antibiotics+5-Fu (AF). Only the control and HEP-only groups had no xenografts. Values are expressed as means ± SDs (n = 8), #P < 0.05 compared with control group, ∗P < 0.05, ∗∗P < 0.01 compared with model group, indicates significant differences compared to the model group.

Figure 5 KRT17 regulates LSCC progression by affecting AKT/mTOR and Wnt/β-catenin axes (a). The protein-protein interaction (PPI) network of the KRT17 from STRING online database (https://string-db.org) was constructed (b). The protein levels in AMC-HN-8 cells were evaluated using western blot (c). The protein levels in TU177 cells were estimated using western blot. ∗A significant difference compared with the sh-NC group. ∗∗P < 0.01 and ∗∗∗P < 0.001.

Figure 7: Ectopic expression of miR-326 in A549 and SPC-A-1 cells reduces cell migration and invasion motility. (E) Expression of MMP-7 and MMP-9 protein in A549 and SPC-A-1 cells after transfection.