产品: GRP94 抗体
货号: AF0735
描述: Rabbit polyclonal antibody to GRP94
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 92kDa; 92kD(Calculated).
蛋白号: P14625
RRID: AB_2834142

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(88%), Sheep(88%), Rabbit(88%), Dog(88%)
克隆:
Polyclonal
特异性:
GRP94 Antibody detects endogenous levels of total GRP94.
RRID:
AB_2834142
引用格式: Affinity Biosciences Cat# AF0735, RRID:AB_2834142.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

94 kDa glucose regulated protein; 94 kDa glucose-regulated protein; ECGP; Endoplasmin; Endothelial cell (HBMEC) glycoprotein; ENPL_HUMAN; Glucose regulated protein 94kDa; gp96; gp96 homolog; GRP 94; GRP-94; Heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; Heat shock protein, 90 kDa, beta, 1; HSP90B1; Stress inducible tumor rejection antigen GP96; TRA1; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; Tumor rejection antigen-1 (gp96);

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
GRP94 Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5.
序列:
MRALWVLGLCCVLLTFGSVRADDEVDVDGTVEEDLGKSREGSRTDDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALSGNEELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVIVTSKHNNDTQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFINFPIYVWSSKTETVEEPMEEEEAAKEEKEESDDEAAVEEEEEEKKPKTKKVEKTVWDWELMNDIKPIWQRPSKEVEEDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAPRGLFDEYGSKKSDYIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKIADDKYNDTFWKEFGTNIKLGVIEDHSNRTRLAKLLRFQSSHHPTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKESREAVEKEFEPLLNWMKDKALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEEMDVGTDEEEETAKESTAEKDEL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
88
Sheep
88
Dog
88
Rabbit
88
Horse
75
Xenopus
75
Chicken
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P14625 作为底物

Site PTM Type Enzyme
S42 Phosphorylation
T44 Phosphorylation
N62 N-Glycosylation
S64 Phosphorylation
K72 Ubiquitination
K75 Acetylation
K75 Ubiquitination
S92 Phosphorylation
Y94 Phosphorylation
K95 Ubiquitination
K97 Acetylation
K97 Ubiquitination
S106 Phosphorylation
N107 N-Glycosylation
S109 Phosphorylation
K114 Acetylation
K114 Ubiquitination
S127 Phosphorylation
T133 Phosphorylation
K135 Ubiquitination
K140 Acetylation
K142 Ubiquitination
T148 Phosphorylation
T150 Phosphorylation
K161 Ubiquitination
T165 Phosphorylation
K168 Ubiquitination
S169 Phosphorylation
T171 Phosphorylation
S172 Phosphorylation
K177 Methylation
T179 Phosphorylation
S187 Phosphorylation
Y200 Phosphorylation
S201 Phosphorylation
N217 N-Glycosylation
S225 Phosphorylation
S227 Phosphorylation
K252 Ubiquitination
S256 Phosphorylation
Y258 Phosphorylation
K265 Ubiquitination
K270 Ubiquitination
Y280 Phosphorylation
T288 Phosphorylation
S306 Phosphorylation
K340 Ubiquitination
S347 Phosphorylation
K348 Ubiquitination
K356 Ubiquitination
K360 Ubiquitination
S385 Phosphorylation
T391 Phosphorylation
S392 Phosphorylation
R395 Methylation
Y401 Phosphorylation
S403 Phosphorylation
K404 Acetylation
K404 Ubiquitination
K405 Ubiquitination
Y408 Phosphorylation
K410 Ubiquitination
Y429 Phosphorylation
K434 Ubiquitination
S439 Phosphorylation
N445 N-Glycosylation
S447 Phosphorylation
K455 Ubiquitination
K458 Ubiquitination
K467 Ubiquitination
T468 Phosphorylation
K473 Ubiquitination
K479 Acetylation
K479 Ubiquitination
K486 Methylation
K486 Ubiquitination
K493 Ubiquitination
S501 Phosphorylation
T504 Phosphorylation
R511 Methylation
S515 Phosphorylation
T519 Phosphorylation
Y527 Phosphorylation
S544 Phosphorylation
S545 Phosphorylation
S551 Phosphorylation
S552 Phosphorylation
K561 Ubiquitination
K586 Acetylation
K586 Sumoylation
K586 Ubiquitination
K593 Ubiquitination
T604 Phosphorylation
K613 Acetylation
K613 Ubiquitination
K623 Ubiquitination
K633 Ubiquitination
T641 Phosphorylation
S643 Phosphorylation
Y652 Phosphorylation
K663 Methylation
K663 Ubiquitination
Y667 Phosphorylation
T669 Phosphorylation
K671 Ubiquitination
S674 Phosphorylation
T675 Phosphorylation
Y677 Phosphorylation
Y678 Phosphorylation
S680 Phosphorylation
K682 Acetylation
K682 Ubiquitination
K683 Ubiquitination
T684 Phosphorylation
R690 Methylation
Y727 Phosphorylation
K733 Ubiquitination
Y735 Phosphorylation
R738 Methylation
R744 Methylation
S746 Phosphorylation
T774 Phosphorylation
T786 Phosphorylation
T792 Phosphorylation

研究背景

功能:

Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity (By similarity).

细胞定位:

Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 (By similarity). Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP (By similarity). Interacts with TLR4 and TLR9, but not with TLR3. Interacts with MZB1 in a calcium-dependent manner (By similarity). Interacts with METTL23.

蛋白家族:

Belongs to the heat shock protein 90 family.

研究领域

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

文献引用

1). Proteomics analysis of serum and urine identifies VCP and CTSA as potential biomarkers associated with multiple myeloma. Clinica chimica acta; international journal of clinical chemistry, 2024 (PubMed: 38081446) [IF=5.0]

2). C1QA and COMP: plasma-based biomarkers for early diagnosis of pancreatic neuroendocrine tumors. Scientific reports, 2023 (PubMed: 38030709) [IF=4.6]

Application: WB    Species: Human    Sample:

Figure 5 Validation of potential protein targets. Western blot profiling and densitometric analysis of final selected proteins (A). Densitometric analysis of (B) C1QA (p = 0.001, 0.03, 0.0013); (C) COMP protein (p = 0.011, 0.019), (D) HSP90B1, (E) ITGA2B/CD41, (F) FN1, (G, H) Sandwich ELISA for C1QA binding analysis (I) ROC curve analysis for plasma C1QA levels in Grade I PanNET vs controls (J, K) Sandwich ELISA for COMP analysis (I) ROC curve analysis for plasma COMP levels in Grade I PanNET vs controls.

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