Fibrillin 1 Antibody - #AF0429

Figure 3. TGF-β1 induces expression of pro-fibrotic molecules. Relative mRNA expression of (a) α-SMA (b) COL6A2 (c) Fibrillin (d) Fibronectin (e) PAI-1 (f) UPR genes XBP1 and CCT4 (g) Protein expression of pro-fibrotic molecules after TGF-β1 treatment with densitometry analysis using ImageJ (h). (i) Vimentin expression after 10 ng/mL TGF-β1 treatment and densitometry analysis (j). The error bars represent standard deviation

Fig. 4 Supplementation of n-3 polyunsaturated fatty acids contributes to improved interstitial fibrosis in rat renal transplantation models. A Schematic view of the establishment of rat renal transplantation models and the dietary mode. B Fatty acid constitution of soybean oil feed (SbOF) and linseed oil feed (LOF). C Gross morphology of bilateral kidneys from SbOF and LOF rats. D, E Masson staining detected the fibrosis level of transplanted kidneys from SbOF and LOF diet rats. F HE staining in heart, liver, spleen, lung, and normal kidney tissues from SbOF and LOF diet rats. G, H IHC detected fibrosis markers in transplanted kidneys from SbOF and LOF diet rats. Significance was determined by Student’s t test.

Figure 2. TGF-induced fibrosis and EMT is regulated by ATG5. (A) The protein levels of ATG-5, LC3-I, LC3-II, SMA, vimentin, fibronectin, and fibrillin in whole-cell lysates of siRNA-mediated knockdown HTM cells with (10 ng/mL) and without TGF-β1. (B–G), relative quantification of the proteins obtained using densitometry analysis of the bands. The error bars represent the standard deviation