产品: MMP3 抗体
货号: AF0217
描述: Rabbit polyclonal antibody to MMP3
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Horse, Sheep, Rabbit, Dog
分子量: 53kDa; 54kD(Calculated).
蛋白号: P08254
RRID: AB_2833347

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Horse(100%), Sheep(87%), Rabbit(92%), Dog(93%)
克隆:
Polyclonal
特异性:
MMP3 Antibody detects endogenous levels of total MMP3.
RRID:
AB_2833347
引用格式: Affinity Biosciences Cat# AF0217, RRID:AB_2833347.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CHDS6; Matrix metalloproteinase 3; Matrix metalloproteinase-3; MGC126102; MGC126103; MGC126104; MMP 3; MMP-3; MMP3; MMP3_HUMAN; Proteoglycanase; SL-1; SL1; STMY; STMY1; STR1; Stromelysin 1; Stromelysin-1; Transin 1; Transin-1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
MMP3 Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Belongs to the peptidase M10A family. Note: This description may include information from UniProtKB.
序列:
MKSLPILLLLCVAVCSAYPLDGAARGEDTSMNLVQKYLENYYDLKKDVKQFVRRKDSGPVVKKIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDVGHFRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Dog
93
Rabbit
92
Sheep
87
Xenopus
55
Pig
36
Bovine
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P08254 作为底物

Site PTM Type Enzyme
Y18 Phosphorylation
Y116 Phosphorylation
Y121 Phosphorylation
K127 Ubiquitination

研究背景

功能:

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

细胞定位:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

研究领域

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

文献引用

1). Pan Z et al. Naringenin protects against iron overload-induced osteoarthritis by suppressing oxidative stress. PHYTOMEDICINE 2022 Oct;105:154330. (PubMed: 35905566) [IF=7.9]

2). Zou ZL et al. Avicularin suppresses cartilage extracellular matrix degradation and inflammation via TRAF6/MAPK activation. PHYTOMEDICINE 2021 Jul 12;91:153657. (PubMed: 34371251) [IF=7.9]

Application: WB    Species: Rat    Sample: chondrocytes

Fig. 2. Effects of avicularin on ECM degradation. (A-D) The mRNA levels of ACAN, Collagen 2, MMP3 and MMP13 in rat and human chondrocytes were detected by qPCR. The dates were shown as the means ± S.D. ** p < 0.01 and *** p < 0.001 compared with the control group; # p < 0.05, ## p < 0.01 and ### p < 0.001 compared with the IL-1β-induced group, n = 3. (E-F) The expression of MMP3, MMP13, ADAMTS5, SOX9, ACAN and Collagen II was detected by western blots.

Application: IHC    Species: Rat    Sample: chondrocytes

Fig. 7. Avicularin attenuates ECM degradation in ACLT rats. (A) ACAN, (B) Collagen II, (C) MMP3, (D) MMP13 and (E) TRAF6 immunohistochemistry of knee joint medial compartment cartilage. (F-J) Quantitative analysis of ACAN, Collagen II, MMP3, MMP13 and TRAF6. The values were shown as the means ± S.D. ** p < 0.01 and *** p < 0.001 compared with the control group; # p < 0.05, ## p < 0.01 and ### p < 0.001 compared with the ACLT group, n = 6. (K) The working model of how avicularin protects against OA development.

3). Li Y et al. Ershiwuwei Lvxue Pill alleviates rheumatoid arthritis by different pathways and produces changes in the gut microbiota. Phytomedicine 2022 Sep 17;107:154462 (PubMed: 36162242) [IF=7.9]

4). Feng K et al. Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity. Biomedicine & Pharmacotherapy 2021 Jun;138:111531 (PubMed: 34311530) [IF=7.5]

5). He Q et al. Biochanin A protects against iron overload associated knee osteoarthritis via regulating iron levels and NRF2/System xc-/GPX4 axis. Biomedicine & Pharmacotherapy 2023 Jan;157:113915. (PubMed: 36379122) [IF=7.5]

6). Ting Wang et al. TGF-β induced PAR-1 expression promotes tumor progression and osteoclast differentiation in giant cell tumor of bone. International Journal of Cancer 2017 Oct 15;141(8):1630-1642 (PubMed: 28670703) [IF=6.4]

Application: IHC    Species: human    Sample: GCTB

Supplementary Figure 1 | The expression of MMP3 in GCT of bone. The expression level of MMP3 showed no significant correlation with TGF-β and PAR-1 expression.

7). Guo Q et al. PDLIM2 protects articular chondrocytes from lipopolysaccharide-induced apoptosis, degeneration and inflammatory injury through down-regulation of nuclear factor (NF)-κB signaling. International Immunopharmacology 2020 Aug 14;88:106883. (PubMed: 32805696) [IF=5.6]

Application: WB    Species: Mouse    Sample: chondrocytes

Fig. 2. Up-regulation of PDLIM2 attenuates LPS-induced injury in chondrocytes. Chondrocytes were transfected with empty vector (EV) or PDLIM2 expression vector for 48 h and then stimulated with 10 μg/ml of LPS for 6 h. (A) The relative mRNA expression of PDLIM2 was determined by RT-qPCR. (B, C) Protein expression of PDLIM2 was examined by Western blotting. (D) The effect of PDLIM2 overexpression on cell viability was detected by MTT assay. The effect of PDLIM2 overexpression on cell apoptosis was assessed by (E, F) TUNENL and (G, H) Annexin V-FITC/PI apoptosis assays. (I) The effect of PDLIM2 overexpression on MMP-3, MMP-13, COL2A1 and ACAN was evaluated by Western blotting. Quantitative analysis of protein expression of (J) MMP-3, (K) MMP-13, (L) COL2A1 and (M) ACAN. N = 3, **p < 0.01 and ***p < 0.001.

8). Jiang-Tao Wan et al. Tumor necrosis factor-α inhibition restores matrix formation by human adipose-derived stem cells in the late stage of chondrogenic differentiation. World Journal of Stem Cells (PubMed: 36483847) [IF=4.1]

9). Wang et al. Deciphering the pharmacological mechanisms of Fraxini Cortex for ulcerative colitis treatment based on network pharmacology and in vivo studies. BMC Complementary Medicine and Therapies 2023 May 09;23(152) (PubMed: 37161415) [IF=3.9]

10). Jie L et al. The mechanism of palmatine-mediated intestinal flora and host metabolism intervention in OA-OP comorbidity rats. Frontiers in Medicine 2023;10(1153360) (PubMed: 37153081) [IF=3.9]

Application: WB    Species: Rat    Sample:

FIGURE 4 Effect of PAL on bone pathological section of Op Oa Rats. (A) Representative pictures of rat knee tissue sections stained with H & E, Saffron O/Fast Green and TRAP. (B) Representative images of protein bands in rat tissues. (C) Representative images of protein bands in rat cartilage. (D) The relative expression levels of MMP3 in cartilage. (E) The relative expression levels of MMP13 in cartilage. (F) The relative expression levels of OPN in femur. (G) The relative expression levels of RUNX-2 in femur. *Statistically significant difference (P < 0.05). **Statistically significant difference (P < 0.01). ***Statistically significant difference (P < 0.001).

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