Collagen III Antibody - #AF0136

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产品: Collagen III 抗体
货号: AF0136
描述: Rabbit polyclonal antibody to Collagen III
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P02461
RRID: AB_2813770

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Collagen III Antibody detects endogenous levels of total Collagen III.
RRID:
AB_2813770
引用格式: Affinity Biosciences Cat# AF0136, RRID:AB_2813770.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Alpha 1 type III collagen; Alpha1 (III) collagen; CO3A1_HUMAN; COL 3A1; COL3A1; Collagen alpha 1(III) chain; Collagen alpha-1(III) chain; Collagen III alpha 1 chain precursor; Collagen III alpha 1 polypeptide; Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant); Collagen type III alpha 1; Collagen type III alpha 1 chain; Collagen type III alpha; Collagen, fetal; EDS4A; Ehlers Danlos syndrome type IV, autosomal dominant; Fetal collagen; Type III collagen;

抗原和靶标

免疫原:

A synthesized peptide derived from human Collagen III, corresponding to a region within N-terminal amino acids.

基因/基因ID:
COL3A1(1281)
描述:
CO3A1 Collagen type III occurs in most soft connective tissues along with type I collagen. Belongs to the fibrillar collagen family. Trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.

研究领域

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Digestive system > Protein digestion and absorption.

文献引用

1). Long-term simulated microgravity fosters carotid aging-like changes via Piezo1. Cardiovascular research, 2024 (PubMed: 38271270) [IF=10.2]
2). An asymmetric chitosan scaffold for tendon tissue engineering: In vitro and in vivo evaluation with rat tendon stem/progenitor cells. Acta Biomaterialia, 2018 (PubMed: 29678676) [IF=9.4]

Application: IF/ICC    Species: rat    Sample: tendon

Fig. 6. | Analyses of tendon tissue by immunofluorescence. Fluorescence immunohistochemistry at the regenerated tendon in each group at 4 weeks. (A-D) The production of COL1; (E-H) The production of COL3; (I-L) The production of TNMD; (M-P) The production of CD31. Blue: DAPI (cell nuclei); Green:COL1/COL3/CD31; Red: TNMD. Scale bar = 100 μm.

Application: IHC    Species: rat    Sample: tendon

Fig. 5.| Detailed analyses of tendon tissue organization and immunohistochemistry. (A-E) DAPI staining and chart of calculated cell density. Data were mean ± S.D. * p < 0.05 vs. ED group. (F-Q)Representative images of regenerated tendon in each group at 4 weeks. Scale bar =100 μm.
3). Exosome from indoleamine 2,3-dioxygenase-overexpressing bone marrow mesenchymal stem cells accelerates repair process of ischemia/reperfusion-induced acute kidney injury by regulating macrophages polarization. Stem cell research & therapy, 2022 (PubMed: 35902956) [IF=7.5]

Application: WB    Species: Mouse    Sample:

Fig. 4 Effect of MSCs-Exo-IDO on renal fibrosis in IRI mouse model. A Kidney tissues were harvested from the four groups on day 28 after IRI (n = 3/group). Kidney tissues in different groups were obtained and histopathology was monitored by Masson staining. Arrowheads indicated the collagen deposition and fibrotic lesions. B Quantization diagram of panel A as shown in the right. C Western Blot was used to detect the expression of α-SMA, collagen I and collagen III in the above kidney tissues (n = 3/group). D Quantization diagram of panel C as shown in the right. The histogram is presented by Means ± SD. *P 
4). Modified citrus pectin ameliorates myocardial fibrosis and inflammation via suppressing galectin-3 and TLR4/MyD88/NF-κB signaling pathway. Biomedicine & Pharmacotherapy, 2020 (PubMed: 32172066) [IF=6.9]

Application: IHC    Species: rat    Sample: heart

Fig. 5. |Effects of MCP on ISO-induced myocardial fibrosis in rats. (A and B) Immunohistochemical analysis of collagen I and collagen Ⅲ protein in heart cross sections of different groups on day 15 and day 22 (200× magnification). Scale bars 100 μm.

Application: WB    Species: rat    Sample: heart

Fig. 6. |Effects of MCP on the expression of collagen Ⅰ and collagen Ⅲ induced by ISO in rats. Protein and mRNA levels of collagen Ⅰ and collagen Ⅲ were analyzed by western blot and qRT-PCR in each group on day 15 and day 22. (A) Protein expression of collagen Ⅰ and collagen Ⅲ were measured by western blot.
5). Gentianella acuta improves TAC-induced cardiac remodelling by regulating the Notch and PI3K/Akt/FOXO1/3 pathways. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2022 (PubMed: 35988427) [IF=6.9]

Application: WB    Species: Rat    Sample:

Fig. 5. G. acuta prevented TAC-induced myocardial fibrosis in rats. (A,B) Masson′s trichrome staining to observe the interstitial collagen deposition in the left ventricle; scale bars = 100 µm, and bar graph showing the percentage of collagen deposition. (C,D) The protein expression levels of α-SMA, collagen I, and collagen III are detected via western blotting and quantitative analysis of the relative protein expression of α-SMA, collagen I, and collagen III. GAPDH is used as a loading control. (n = 3). (E-G) The mRNA levels of α-SMA, collagen I, and collagen III are measured by Real-time PCR in each group, and relative mRNA levels normalise to GAPDH (n = 6). Significance: * p 
6). Amphibian-derived peptide RL-RF10 ameliorates paraquat-induced pulmonary fibrosis injury. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38244328) [IF=6.9]
7). Protective role of Gentianella acuta on isoprenaline induced myocardial fibrosis in rats via inhibition of NF-κB pathway. BIOMEDICINE & PHARMACOTHERAPY, 2019 (PubMed: 30554111) [IF=6.9]
8). Ethyl ferulate suppresses post-myocardial infarction myocardial fibrosis by inhibiting transforming growth factor receptor 1. Phytomedicine, 2023 (PubMed: 37801895) [IF=6.7]
9). Targeting the Akt/PI3K/mTOR signaling pathway for complete eradication of keloid disease by sunitinib. Apoptosis, 2022 (PubMed: 35802302) [IF=6.1]
10). Bi-layered Composite Scaffold for Repair of the Osteochondral Defects. Advances in Wound Care, 2021 (PubMed: 33076773) [IF=5.8]
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