Collagen I Antibody - #AF0134

Fig. 5. |Effects of MCP on ISO-induced myocardial fibrosis in rats. (A and B) Immunohistochemical analysis of collagen I and collagen Ⅲ protein in heart cross sections of different groups on day 15 and day 22 (200× magnification). Scale bars 100 μm.

Fig. 6. |Effects of MCP on the expression of collagen Ⅰ and collagen Ⅲ induced by ISO in rats. Protein and mRNA levels of collagen Ⅰ and collagen Ⅲ were analyzed by western blot and qRT-PCR in each group on day 15 and day 22. (A) Protein expression of collagen Ⅰ and collagen Ⅲ were measured by western blot.

Figure 6.|Effect of PTPN2 overexpression on SiO2 stimulated MLE‐12 cells.(A)Immunofluorescent staining for PTPN2 and E‐cad expression in NC‐LW299 and LW1049 cells with SiO2; Scale bars: 50μm. (B) Immunofluorescent staining for Vimentin expression in NC‐LW299 and LW1049 cells with SiO2; Scale bars: 50 μm. (C) Western blot for the expression levels of PTPN2, E‐cad, Vimentin, and p‐STAT3 in NC‐LW299 and LW1049 cells with SiO2

Figure 3. Collagen I expression in the lung tissue of LPS-challenged mice without treatment and after Pulmozyme administration. (A). Representative immunohistochemical images of the lung sections of the control and experimental groups stained with anti-collagen I primary antibodies. Original magnification ×200. Green arrows indicate collagen I in the lungs. (B). The effect of Pulmozyme on collagen I expression in the lungs. The intensity of collagen I expression in the lungs of the control (black curves) and experimental (red curves) groups was assessed by a semiquantitative method, where 0—no pathological changes, 1—mild collagen I expression, 2—moderate collagen I expression, and 3—severe collagen I expression. Dotted lines show collagen I expression in the lungs of healthy animals. Statistically significant differences at * p < 0.05 relative to the control.

Fig. 3| MiR-185-5p prevents extracellular matrix accumulation and dedifferentiation of TGF-β1-induced HK2 cells by downregulating ATF6. a Expression of fibronectin, collagen I, and collagen III, and quantitative analysis of relative protein expression.

Fig. 9. Effects of ONSMP on downstream proteins of MAPK signaling pathway. (A) Representative western blotting bands of p-c-Fos, p-c-Jun, LOX, CCND1, COL Ⅰ, COL Ⅲ, and α-SMA in the myocardium, with vinculin, and GAPDH as internal reference. (B–K) Statistical graphs of p-c-Fos, p-c-Jun, LOX, CCND1, COL Ⅰ, COL Ⅲ, and α-SMA (n = 3). Data are presented as mean ± SEM. nsP > 0.05; ▲▲P < 0.01 vs the sham; *P < 0.05, **P < 0.01 vs the model; ##P < 0.01 vs the ONSMP-L; ※P < 0.05, ※※P < 0.01 vs the ONSMP-M.

Figure 2. A characterization of the native and decellularized human umbilical cord (UC-scaffold): (a) native tissue H&E staining (scale bar = 100 µm); (b) the UC-scaffold H&E staining (scale bar = 100 µm); (c) DNA content, ng per 1 mg of dry weight; (d) the macroscopic appearance of the scaffold; (e) scanning electron microscopy (SEM) image of the scaffold (scale bar = 1 mm); (f) transmission electronic microscopy (TEM) image of the native tissue (scale bar = 200 nm); (g) TEM image of the native tissue (scale bar = 100 nm); (h) TEM image of the scaffold (scale bar = 200 nm); (i) TEM image of the native tissue (scale bar = 100 nm); (j) content of type I collagen as measured via Western blotting; (k) native tissue Alcian blue staining (scale bar = 100 µm); (l) the UC-scaffold Alcian blue staining (scale bar = 100 µm); (m) Alcian blue staining after the UC-scaffold treatment with hyaluronidase (scale bar = 100 µm); (n) GAG content, µg per 1 mg of dry weight; (o) immunohistochemical staining for type IV collagen (scale bar = 100 µm); (p) immunohistochemical staining for laminin (scale bar = 100 µm); (q) immunohistochemical staining for fibronectin (scale bar = 100 µm); (r) content of TGF-β3 as measured via Western blotting; (s) content of VEGF as measured via Western blotting; (t) scaffold with hADSCs, 7 days of cultivation, LIVE/DEAD staining (scale bar = 100 µm); and (u) scaffold with hADSCs, 14 days of cultivation, LIVE/DEAD staining (scale bar = 100 µm).