APC Antibody - #AF0547

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产品: APC 抗体
货号: AF0547
描述: Rabbit polyclonal antibody to APC
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse
蛋白号: P25054
RRID: AB_2834125

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
APC Antibody detects endogenous levels of total APC .
RRID:
AB_2834125
引用格式: Affinity Biosciences Cat# AF0547, RRID:AB_2834125.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Adenomatous Polyposis Coli; Adenomatous polyposis coli protein; Apc; APC_HUMAN; CC1; Deleted in polyposis 2.5; DP2; DP2.5; DP3; FAP; FPC; GS; Protein APC;

抗原和靶标

免疫原:

A synthesized peptide derived from human APC , corresponding to a region within the internal amino acids.

基因/基因ID:
APC(324)
描述:
This gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

文献引用

1). Effects of 17β-trenbolone exposure on sex hormone synthesis and social behaviours in adolescent mice. Chemosphere, 2020 (PubMed: 31869672) [IF=8.1]

Application: IF/ICC    Species: mice    Sample: medial prefrontal cortex (mPFC)

Fig. 5. Effect of 17ββββ-TBOH on the myelination of the mPFC in adolescent mice. (A) Quantitative 893 real-time PCR (qRT-PCR) of myelin gene transcripts in the mPFC (n=4). (B-C) Confocal images and 894 quantification of MBP+ (green) myelinated fibres in the mPFC. DAPI (blue) was used as a nuclear 895 counterstain (n=12 fields from 3 mice per treatment condition). (D-F) Confocal images and 89， quantification of NG2+ (green) cells and CC1+ (red) cells in the mPFC. DAPI (blue) was used as a 897 nuclear counterstain (n=12 fields from 3 mice per treatment condition). Scale bar=60 µm. *p<0.05 **P 898 < 0.01, ***P < 0.001, and ****P < 0.0001 by one-way ANOVA followed by Tukey’s post hoc test. 899 Data are presented as means ± SEM.
2). Fam70A Binds Wnt5a to Regulate Meiosis and Quality of Mouse Oocytes. Cell Proliferation, 2020 (PubMed: 32391621) [IF=5.9]

Application: WB    Species: mice    Sample: Oocytes

FIGURE 5 Fam70A and Wnt5a regulate adenomatous polyposis coli (APC) level and MT stability. A, Western blot showed that APC protein remarkably increased after Fam70A depletion. B, Quantification of (A). C, Immunofluorescence showed that APC was enriched within spindles and remarkably increased after Fam70A depletion. APC in green, tubulin in red, DNA in blue. D, Western blot showed that Wnt5a was efficiently reduced by siRNA (Table S2). E, Western blot showed that APC remarkably increased after Wnt5a knockdown. F, Quantification of (E). G, RT-PCR showed that APC was efficiently reduced by siRNA (Table S3). H, Fam70A depletion and Wnt5a knockdown both remarkably increased acetylated tubulin level within spindles, while APC knockdown remarkably reduced acetylated tubulin level. Acetylated tubulin in green, DNA in blue. I, Quantification of (H). Scale bar, 5 µm. *P < .05

Application: IF/ICC    Species: mice    Sample: Oocytes

FIGURE 5 Fam70A and Wnt5a regulate adenomatous polyposis coli (APC) level and MT stability. A, Western blot showed that APC protein remarkably increased after Fam70A depletion. B, Quantification of (A). C, Immunofluorescence showed that APC was enriched within spindles and remarkably increased after Fam70A depletion. APC in green, tubulin in red, DNA in blue. D, Western blot showed that Wnt5a was efficiently reduced by siRNA (Table S2). E, Western blot showed that APC remarkably increased after Wnt5a knockdown. F, Quantification of (E). G, RT-PCR showed that APC was efficiently reduced by siRNA (Table S3). H, Fam70A depletion and Wnt5a knockdown both remarkably increased acetylated tubulin level within spindles, while APC knockdown remarkably reduced acetylated tubulin level. Acetylated tubulin in green, DNA in blue. I, Quantification of (H). Scale bar, 5 µm. *P < .05
3). Cannabidiol inhibits invasion and metastasis in colorectal cancer cells by reversing epithelial–mesenchymal transition through the Wnt/β-catenin signaling pathway. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2023 (PubMed: 35960375) [IF=2.7]
4). Cannabidiol inhibits invasion and metastasis in colorectal cancer cells by reversing epithelial-mesenchymal transition through the Wnt/β-catenin signaling pathway. Journal of cancer research and clinical oncology, 2023 (PubMed: 35960375) [IF=2.7]

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