MMP19 Antibody - #AF0215

Fig. 1 MMP19 is significantly upregulated in the endothelial cells of IPF patients and BLM-induced fibrotic mouse lungs. A Immunohistochemistry analysis for MMP19 in IPF lung tissue. B MMP19 expression level in lung BAL fluid of IPF and normal control. C qRT-PCR was performed for the expression of MMP19 in lung homogenates from mice treated with saline or BLM. D Immunoblot analysis of MMP19 in lung homogenates from mice treated with saline or BLM. E Immunohistochemical evaluation of MMP19 in saline control or BLM-challenged mice lung. F Immunohistochemistry analysis for MMP19 in endothelial cells of IPF and BLM-challenged mice lung sections. G Immunofluorescence analysis for CD31 (pink) and MMP19 (green) in endothelial cells in mice treated with saline control or BLM

Fig. 1 MMP19 is significantly upregulated in the endothelial cells of IPF patients and BLM-induced fibrotic mouse lungs. A Immunohistochemistry analysis for MMP19 in IPF lung tissue. B MMP19 expression level in lung BAL fluid of IPF and normal control. C qRT-PCR was performed for the expression of MMP19 in lung homogenates from mice treated with saline or BLM. D Immunoblot analysis of MMP19 in lung homogenates from mice treated with saline or BLM. E Immunohistochemical evaluation of MMP19 in saline control or BLM-challenged mice lung. F Immunohistochemistry analysis for MMP19 in endothelial cells of IPF and BLM-challenged mice lung sections. G Immunofluorescence analysis for CD31 (pink) and MMP19 (green) in endothelial cells in mice treated with saline control or BLM

Fig. 6 MMP19 induces monocyte infiltration is associated with the SDF1/CXCR4 axis. A Western blot was performed for SDF1 in lung homogenates that MMP19WT and MMP19E213A-AAV-infected mice treated with saline control or BLM. B Representative micrographs of immunohistochemical staining for SDF1 and CXCR4 in lung sections (up and middle), and CXCR4 in blood vessels (bottom). C SDF1 expression level in lung endothelial cells of mice treated with saline and BLM. D Expression relevance between MMP19 and SDF1 in lung endothelial cells of mice treated with saline and BLM. E Expression relevance between MMP19 and CXCR4 in IPF lung tissues. F MMP19 and SDF1 were assessed by immunoprecipitation in HPMECs. G Immunofluorescence was performed to localize MMP19 (red) and SDF-1 (green) in endothelial cells in mice and IPF lung tissues. H THP-1 cell adhesion to HPMECs was assessed using an inverted fluorescence microscope. I Lung hydroxyproline levels in MMP19WT-AAV-infected mice treated with BLM and AMD3100. n = 5 in all groups. J Micrographs of immunohistochemical staining of lung sections for EMR1. K Representative micrographs of H&E staining and Masson’s trichrome of lung sections