产品: TOP2A 抗体
货号: AF0793
描述: Rabbit polyclonal antibody to TOP2A
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Sheep, Rabbit, Dog
分子量: 174kDa; 174kD(Calculated).
蛋白号: P11388
RRID: AB_2834111

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Sheep(100%), Rabbit(80%), Dog(100%)
克隆:
Polyclonal
特异性:
TOP2A Antibody detects endogenous levels of total TOP2A.
RRID:
AB_2834111
引用格式: Affinity Biosciences Cat# AF0793, RRID:AB_2834111.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

alpha isozyme; ATP hydrolyzing DNA topoisomerase II alfa; DNA gyrase; DNA topoisomerase (ATP hydrolyzing); DNA topoisomerase 2 alpha; DNA topoisomerase 2-alpha; DNA topoisomerase II 170 kD; DNA topoisomerase II alpha isozyme; DNA topoisomerase II; DNA Topoisomerase2; TOP 2A; TOP2; TOP2A; TOP2A_HUMAN; Topoisomerase DNA II alpha 170kDa; TP2A;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; TOP2; TOP2A; topoisomerase (DNA) II alpha 170kDa; TP2A
序列:
MEVSPLQPVNENMQVNKIKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNSLKVIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSGPTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAVEAKEKQDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Rabbit
80
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P11388 作为底物

Site PTM Type Enzyme
M1 Acetylation
S4 Phosphorylation
S29 Phosphorylation P05129 (PRKCG) , P05771 (PRKCB)
Y82 Phosphorylation
K96 Ubiquitination
K101 Ubiquitination
K123 Ubiquitination
K131 Sumoylation
K131 Ubiquitination
Y151 Phosphorylation
K156 Ubiquitination
K157 Ubiquitination
K168 Ubiquitination
K191 Ubiquitination
K225 Ubiquitination
K227 Sumoylation
K227 Ubiquitination
S230 Phosphorylation
K233 Ubiquitination
S249 Phosphorylation
T250 Phosphorylation
K251 Ubiquitination
K254 Acetylation
K261 Ubiquitination
K265 Ubiquitination
K276 Ubiquitination
K278 Sumoylation
K278 Ubiquitination
T282 Phosphorylation
S285 Phosphorylation
K287 Ubiquitination
S312 Phosphorylation
K321 Ubiquitination
Y328 Phosphorylation
K336 Ubiquitination
K342 Ubiquitination
K352 Ubiquitination
K386 Sumoylation
K386 Ubiquitination
K397 Acetylation
K397 Ubiquitination
K418 Ubiquitination
K425 Ubiquitination
K431 Ubiquitination
K440 Sumoylation
K440 Ubiquitination
S452 Phosphorylation
C455 S-Nitrosylation
S464 Phosphorylation
K466 Ubiquitination
S471 Phosphorylation
K480 Ubiquitination
K489 Sumoylation
K489 Ubiquitination
K499 Ubiquitination
K512 Ubiquitination
Y518 Phosphorylation
K519 Ubiquitination
K520 Ubiquitination
Y522 Phosphorylation
K529 Ubiquitination
K535 Ubiquitination
K579 Ubiquitination
K584 Ubiquitination
K599 Sumoylation
K599 Ubiquitination
Y612 Phosphorylation
K614 Ubiquitination
K622 Ubiquitination
K625 Sumoylation
K625 Ubiquitination
K632 Sumoylation
K632 Ubiquitination
K639 Methylation
K639 Sumoylation
K639 Ubiquitination
K655 Ubiquitination
K656 Ubiquitination
K662 Sumoylation
K662 Ubiquitination
R672 Methylation
K676 Sumoylation
K676 Ubiquitination
S707 Phosphorylation
S709 Phosphorylation
S714 Phosphorylation
S717 Phosphorylation
K723 Ubiquitination
K728 Ubiquitination
K735 Ubiquitination
Y805 Phosphorylation
T825 Phosphorylation
K827 Ubiquitination
Y830 Phosphorylation
K893 Ubiquitination
T898 Phosphorylation
K936 Methylation
K936 Ubiquitination
K949 Ubiquitination
K967 Ubiquitination
K971 Acetylation
K971 Ubiquitination
K976 Ubiquitination
K987 Ubiquitination
K1011 Ubiquitination
R1026 Methylation
K1028 Ubiquitination
S1045 Phosphorylation
K1047 Ubiquitination
K1058 Ubiquitination
K1062 Ubiquitination
K1068 Ubiquitination
K1070 Ubiquitination
K1075 Sumoylation
K1075 Ubiquitination
K1088 Ubiquitination
S1106 Phosphorylation
T1112 Phosphorylation
K1114 Ubiquitination
T1119 Phosphorylation
K1151 Ubiquitination
T1157 Phosphorylation
K1159 Ubiquitination
S1162 Phosphorylation
S1164 Phosphorylation
K1184 Ubiquitination
K1186 Ubiquitination
K1196 Acetylation
K1196 Sumoylation
K1196 Ubiquitination
K1199 Acetylation
K1201 Acetylation
K1204 Sumoylation
K1204 Ubiquitination
T1205 Phosphorylation
S1213 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3) , P06493 (CDK1)
K1228 Sumoylation
K1228 Ubiquitination
K1233 Acetylation
K1234 Acetylation
K1237 Acetylation
K1240 Sumoylation
K1240 Ubiquitination
T1244 Phosphorylation
S1247 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1) , P06493 (CDK1)
K1259 Ubiquitination
T1272 Phosphorylation
T1274 Phosphorylation
K1276 Acetylation
K1276 Ubiquitination
K1283 Methylation
K1287 Acetylation
K1289 Acetylation
S1295 Phosphorylation
S1297 Phosphorylation
S1299 Phosphorylation
S1302 Phosphorylation
S1303 Phosphorylation
S1306 Phosphorylation
T1324 Phosphorylation
T1327 Phosphorylation
S1332 Phosphorylation
S1337 Phosphorylation P53350 (PLK1)
T1343 Phosphorylation P68400 (CSNK2A1) , P53350 (PLK1) , Q9H4B4 (PLK3)
S1351 Phosphorylation
S1354 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1) , P06493 (CDK1)
T1358 Phosphorylation
T1360 Phosphorylation
S1361 Phosphorylation P06493 (CDK1) , P28482 (MAPK1) , P27361 (MAPK3) , P49841 (GSK3B)
S1365 Phosphorylation P68400 (CSNK2A1)
K1367 Acetylation
K1367 Ubiquitination
S1374 Phosphorylation
S1377 Phosphorylation P68400 (CSNK2A1)
K1385 Sumoylation
K1385 Ubiquitination
S1387 Phosphorylation
S1391 Phosphorylation
S1392 Phosphorylation
S1393 Phosphorylation P06493 (CDK1) , P28482 (MAPK1) , P27361 (MAPK3)
T1397 Phosphorylation
T1403 Phosphorylation
T1406 Phosphorylation
K1422 Ubiquitination
S1426 Phosphorylation
T1429 Phosphorylation
T1430 Phosphorylation
K1442 Sumoylation
K1442 Ubiquitination
R1443 Methylation
S1449 Phosphorylation
S1452 Phosphorylation
K1454 Acetylation
K1454 Ubiquitination
K1459 Ubiquitination
K1461 Ubiquitination
K1467 Acetylation
S1469 Phosphorylation P68400 (CSNK2A1)
T1470 Phosphorylation
S1471 Phosphorylation
S1474 Phosphorylation
S1476 Phosphorylation
K1480 Acetylation
K1484 Ubiquitination
S1491 Phosphorylation
K1492 Sumoylation
K1492 Ubiquitination
S1495 Phosphorylation
S1504 Phosphorylation
Y1521 Phosphorylation
S1525 Phosphorylation P68400 (CSNK2A1) , P53350 (PLK1) , P53778 (MAPK12)

研究背景

功能:

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).

翻译修饰:

Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.

细胞定位:

Cytoplasm. Nucleus>Nucleoplasm.
Note: Generally located in the nucleoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer. Interacts with COPS5. Interacts with RECQL5; this stimulates DNA decatenation. Interacts with SETMAR; stimulates the topoisomerase activity. Interacts with DHX9; this interaction occurs in a E2 enzyme UBE2I- and RNA-dependent manner, negatively regulates DHX9-mediated double-stranded DNA and RNA duplex helicase activity and stimulates TOP2A-mediated supercoiled DNA relaxation activity. Interacts with HNRNPU (via C-terminus); this interaction protects the topoisomerase TOP2A from degradation and positively regulates the relaxation of supercoiled DNA in a RNA-dependent manner (By similarity). Interacts with MCM3AP isoform GANP. Interacts with ERCC6.

蛋白家族:

The N-terminus has several structural domains; the ATPase domain (about residues 1-265), the transducer domain (about 266-428) and the toprim domain (455-572) (PubMed:25202966). Comparing different structures shows ATP hydrolysis induces domain shifts in the N-terminus that are probably part of the mechanism of DNA cleavage and rejoining (PubMed:25202966).

Belongs to the type II topoisomerase family.

研究领域

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

文献引用

1). Sharp Downregulation of Hub Genes Associated With the Pathogenesis of Breast Cancer From Ductal Carcinoma In Situ to Invasive Ductal Carcinoma. Frontiers in Oncology (PubMed: 34094915) [IF=4.7]

Application: WB    Species: Human    Sample: breast cancer tissue

Figure 6 Protein expression levels of hub genes. (A) Protein expression levels of CDK1, MELK, CEP55, TOP2A, NUSAP1, PBK, RRM2, and MAD2L1 were determined by western blotting. (B) Western blot analysis of CDK1, MELK, CEP55, TOP2A, NUSAP1, PBK, RRM2, and MAD2L1 in tissue from different stages of breast disease, and quantification of the intensity relative to GAPDH. One-way ANOVA was performed to acquire statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). NME, normal mammary epithelium; SH, simple ductal hyperplasia; ADH, atypical ductal hyperplasia; DCIS, ductal carcinoma in situ; IDC, invasive ductal carcinoma.

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