产品: MMP14 抗体
货号: AF0212
描述: Rabbit polyclonal antibody to MMP14
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 55kd, 65kDa; 66kD(Calculated).
蛋白号: P50281
RRID: AB_2833344

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(88%), Dog(88%)
克隆:
Polyclonal
特异性:
MMP14 Antibody detects endogenous levels of total MMP14.
RRID:
AB_2833344
引用格式: Affinity Biosciences Cat# AF0212, RRID:AB_2833344.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Matrix metallopeptidase 14 (membrane inserted); Matrix metalloproteinase 14; Matrix metalloproteinase-14; Membrane type 1 matrix metalloproteinase; Membrane type 1 metalloprotease; Membrane type matrix metalloproteinase 1; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP 14; MMP X1; MMP-14; MMP-X1; Mmp14; MMP14_HUMAN; MMPX1; MT MMP 1; MT-MMP 1; MT1 MMP; MT1-MMP; MT1MMP; MTMMP 1; MTMMP1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P50281 MMP14_HUMAN:

Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.

描述:
MMP14 Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. Belongs to the peptidase M10A family.
序列:
MSPAPRPPRCLLLPLLTLGTALASLGSAQSSSFSPEAWLQQYGYLPPGDLRTHTQRSPQSLSAAIAAMQKFYGLQVTGKADADTMKAMRRPRCGVPDKFGAEIKANVRRKRYAIQGLKWQHNEITFCIQNYTPKVGEYATYEAIRKAFRVWESATPLRFREVPYAYIREGHEKQADIMIFFAEGFHGDSTPFDGEGGFLAHAYFPGPNIGGDTHFDSAEPWTVRNEDLNGNDIFLVAVHELGHALGLEHSSDPSAIMAPFYQWMDTENFVLPDDDRRGIQQLYGGESGFPTKMPPQPRTTSRPSVPDKPKNPTYGPNICDGNFDTVAMLRGEMFVFKERWFWRVRNNQVMDGYPMPIGQFWRGLPASINTAYERKDGKFVFFKGDKHWVFDEASLEPGYPKHIKELGRGLPTDKIDAALFWMPNGKTYFFRGNKYYRFNEELRAVDSEYPKNIKVWEGIPESPRGSFMGSDEVFTYFYKGNKYWKFNNQKLKVEPGYPKSALRDWMGCPSGGRPDEGTEEETEVIIIEVDEEGGGAVSAAAVVLPVLLLLLVLAVGLAVFFFRRHGTPRRLLYCQRSLLDKV

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
88
Sheep
88
Dog
88
Rabbit
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P50281 作为底物

Site PTM Type Enzyme
T77 O-Glycosylation
T77 Phosphorylation
Y138 Phosphorylation
T140 Phosphorylation
Y141 Phosphorylation
T291 O-Glycosylation
T299 O-Glycosylation
T300 O-Glycosylation
S301 O-Glycosylation
S304 O-Glycosylation
Y399 Phosphorylation
S462 Phosphorylation
T567 Phosphorylation P05129 (PRKCG)
Y573 Phosphorylation P12931 (SRC) , P53667 (LIMK1)
S577 Phosphorylation
K581 Ubiquitination

研究背景

功能:

Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (By similarity). May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis.

翻译修饰:

The precursor is cleaved by a furin endopeptidase.

Tyrosine phosphorylated by PKDCC/VLK.

细胞定位:

Membrane>Single-pass type I membrane protein. Melanosome. Cytoplasm.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.

亚基结构:

Interacts (via C-terminal cytoplasmic tail) with BST2. Interacts with DLL1; inhibits DLL1-induced Notch signaling.

蛋白家族:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

研究领域

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

文献引用

1). Liu J et al. Multifunctional Gomisin B enhances cognitive function in APP/PS1 transgenic mice by regulating Aβ clearance and neuronal apoptosis. Biomedicine & Pharmacotherapy 2023 Sep 04;166(115423) (PubMed: 37673021) [IF=7.5]

2). Bourebaba L et al. The PTP1B Inhibitor Trodusquemine (MSI-1436) Improves Glucose Uptake in Equine Metabolic Syndrome Affected Liver through Anti-Inflammatory and Antifibrotic Activity. International Journal of Inflammation 2023;2023(3803056) (PubMed: 37808009) [IF=2.0]

Application: WB    Species: Horse    Sample: liver

Figure 4 MSI-1436 regulates proteolytic ECM remodeling in EMS livers. (a) Relative gene expression of master metalloproteinases. (b) Relative protein expression of ECM-associated MMPs. Results were normalized to the expression of endogenous β-actin control. (c) Expression of metallopeptidase inhibitors 1 and 2. Representative data from three independent experiments are shown ± SD (n = 3). An asterisk (∗) indicates a comparison among healthy, EMS, and EMS-treated groups. ∗p 

3). Zhu et al. Biotransformation of Graphene Oxide in Lung Fluids Enhances its Synergistic Cytotoxic Effect with Cadmium.

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