MMP14 Antibody - #AF0212

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产品: MMP14 抗体
货号: AF0212
描述: Rabbit polyclonal antibody to MMP14
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: P50281
RRID: AB_2833344

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
MMP14 Antibody detects endogenous levels of total MMP14.
RRID:
AB_2833344
引用格式: Affinity Biosciences Cat# AF0212, RRID:AB_2833344.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Matrix metallopeptidase 14 (membrane inserted); Matrix metalloproteinase 14; Matrix metalloproteinase-14; Membrane type 1 matrix metalloproteinase; Membrane type 1 metalloprotease; Membrane type matrix metalloproteinase 1; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP 14; MMP X1; MMP-14; MMP-X1; Mmp14; MMP14_HUMAN; MMPX1; MT MMP 1; MT-MMP 1; MT1 MMP; MT1-MMP; MT1MMP; MTMMP 1; MTMMP1;

抗原和靶标

免疫原:

A synthesized peptide derived from human MMP14, corresponding to a region within C-terminal amino acids.

基因/基因ID:
MMP14(4323)
描述:
MMP14 Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. Belongs to the peptidase M10A family.

研究领域

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

文献引用

1). Multifunctional Gomisin B enhances cognitive function in APP/PS1 transgenic mice by regulating Aβ clearance and neuronal apoptosis. Biomedicine & Pharmacotherapy, 2023 (PubMed: 37673021) [IF=6.9]
2). MMP14 as a central mediator of TGF-β1-induced extracellular matrix remodeling in graves' orbitopathy. Frontiers in endocrinology, 2025 (PubMed: 40766297) [IF=3.9]

Application: WB    Species: human    Sample:

Figure 2. MMP14 expression in orbital tissues correlates with fibrotic severity in GO (A) Representative immunohistochemical detection of MMP14 in orbital tissue sections from NC, GO type I, and GO type II patients. MMP14 immunoreactivity appears as yellowish-brown chromogenic signal. Scale bars: 100 μm. (B) Semi-quantitative analysis of MMP14 immunopositivity across experimental groups (NC: n=5; GO type I: n=7; GO type II: n=7). (C) Representative immunoblot analysis of MMP14 and α-SMA protein expression in orbital adipose/connective tissue specimens from normal controls and GO patients with varying fibrotic grades. (D) Densitometric quantification of protein expression normalized to GAPDH. Values represent mean ± SD from three independent experiments. Statistical significance was determined by one-way ANOVA with post-hoc analysis: ns, not significant; ####p

Application: IHC    Species: human    Sample:

Figure 2. MMP14 expression in orbital tissues correlates with fibrotic severity in GO (A) Representative immunohistochemical detection of MMP14 in orbital tissue sections from NC, GO type I, and GO type II patients. MMP14 immunoreactivity appears as yellowish-brown chromogenic signal. Scale bars: 100 μm. (B) Semi-quantitative analysis of MMP14 immunopositivity across experimental groups (NC: n=5; GO type I: n=7; GO type II: n=7). (C) Representative immunoblot analysis of MMP14 and α-SMA protein expression in orbital adipose/connective tissue specimens from normal controls and GO patients with varying fibrotic grades. (D) Densitometric quantification of protein expression normalized to GAPDH. Values represent mean ± SD from three independent experiments. Statistical significance was determined by one-way ANOVA with post-hoc analysis: ns, not significant; ####p
3). The PTP1B Inhibitor Trodusquemine (MSI-1436) Improves Glucose Uptake in Equine Metabolic Syndrome Affected Liver through Anti-Inflammatory and Antifibrotic Activity. International Journal of Inflammation, 2023 (PubMed: 37808009) [IF=2.6]

Application: WB    Species: Horse    Sample: liver

Figure 4 MSI-1436 regulates proteolytic ECM remodeling in EMS livers. (a) Relative gene expression of master metalloproteinases. (b) Relative protein expression of ECM-associated MMPs. Results were normalized to the expression of endogenous β-actin control. (c) Expression of metallopeptidase inhibitors 1 and 2. Representative data from three independent experiments are shown ± SD (n = 3). An asterisk (∗) indicates a comparison among healthy, EMS, and EMS-treated groups. ∗p 
4). Biotransformation of Graphene Oxide in Lung Fluids Enhances its Synergistic Cytotoxic Effect with Cadmium. , 2022

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