规格 价格 库存
 50ul RMB¥ 1300 现货
 100ul RMB¥ 2400 现货
 200ul RMB¥ 3200 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-p95/NBS1 (Ser278) Antibody detects endogenous levels of p95/NBS1 only when phosphorylated at Sersine 278.
RRID:
AB_2834087
引用格式: Affinity Biosciences Cat# AF0830, RRID:AB_2834087.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AT V1; AT V2; ATV; Cell cycle regulatory protein p95; FLJ10155; MGC87362; Nbn; NBN_HUMAN; NBS 1; NBS; NBS1; Nibrin; Nijmegen breakage syndrome 1 (nibrin); Nijmegen breakage syndrome; Nijmegen breakage syndrome protein 1; p95; p95 protein of the MRE11/RAD50 complex;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
O60934 NBN_HUMAN:

Ubiquitous. Expressed at high levels in testis.

描述:
NBS1 is a member of the MRE11/RAD50 double-strand break repair complex. Involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Mutation results in the Nijmegen breakage syndrome (NBS), an autosomal recessive chromosomal instability syndrome.
序列:
MWKLLPAAGPAGGEPYRLLTGVEYVVGRKNCAILIENDQSISRNHAVLTANFSVTNLSQTDEIPVLTLKDNSKYGTFVNEEKMQNGFSRTLKSGDGITFGVFGSKFRIEYEPLVACSSCLDVSGKTALNQAILQLGGFTVNNWTEECTHLVMVSVKVTIKTICALICGRPIVKPEYFTEFLKAVESKKQPPQIESFYPPLDEPSIGSKNVDLSGRQERKQIFKGKTFIFLNAKQHKKLSSAVVFGGGEARLITEENEEEHNFFLAPGTCVVDTGITNSQTLIPDCQKKWIQSIMDMLQRQGLRPIPEAEIGLAVIFMTTKNYCDPQGHPSTGLKTTTPGPSLSQGVSVDEKLMPSAPVNTTTYVADTESEQADTWDLSERPKEIKVSKMEQKFRMLSQDAPTVKESCKTSSNNNSMVSNTLAKMRIPNYQLSPTKLPSINKSKDRASQQQQTNSIRNYFQPSTKKRERDEENQEMSSCKSARIETSCSLLEQTQPATPSLWKNKEQHLSENEPVDTNSDNNLFTDTDLKSIVKNSASKSHAAEKLRSNKKREMDDVAIEDEVLEQLFKDTKPELEIDVKVQKQEEDVNVRKRPRMDIETNDTFSDEAVPESSKISQENEIGKKRELKEDSLWSAKEISNNDKLQDDSEMLPKKLLLTEFRSLVIKNSTSRNPSGINDDYGQLKNFKKFKKVTYPGAGKLPHIIGGSDLIAHHARKNTELEEWLRQEMEVQNQHAKEESLADDLFRYNPYLKRRR

翻译修饰 - O60934 作为底物

Site PTM Type Enzyme
K3 Acetylation
K3 Ubiquitination
Y16 Phosphorylation
Y24 Phosphorylation
S58 Phosphorylation
T60 Phosphorylation
K73 Ubiquitination
K82 Ubiquitination
K188 Ubiquitination
S195 Phosphorylation
Y197 Phosphorylation
K208 Acetylation
K208 Ubiquitination
K233 Acetylation
S278 Phosphorylation Q13315 (ATM)
S292 Phosphorylation
K334 Acetylation
K334 Ubiquitination
T335 Phosphorylation
T336 Phosphorylation
T337 Phosphorylation
S341 Phosphorylation
S343 Phosphorylation Q99986 (VRK1) , Q13535 (ATR) , Q13315 (ATM)
S347 Phosphorylation
T367 Phosphorylation
K388 Methylation
S397 Phosphorylation Q13315 (ATM)
T402 Phosphorylation
S406 Phosphorylation
S418 Phosphorylation
T420 Phosphorylation
K423 Ubiquitination
Y429 Phosphorylation
S432 Phosphorylation P24941 (CDK2)
T434 Phosphorylation
S438 Phosphorylation
K441 Acetylation
S442 Phosphorylation
S447 Phosphorylation
S462 Phosphorylation
C478 S-Nitrosylation
T485 Phosphorylation
S488 Phosphorylation
T493 Phosphorylation
T497 Phosphorylation
S499 Phosphorylation
K504 Acetylation
S509 Phosphorylation
T516 Phosphorylation
S518 Phosphorylation
T524 Phosphorylation
K529 Ubiquitination
S535 Phosphorylation
K544 Acetylation
K550 Acetylation
K568 Acetylation
K582 Sumoylation
T602 Phosphorylation
S604 Phosphorylation
S611 Phosphorylation
S612 Phosphorylation
S615 Phosphorylation Q13535 (ATR) , Q13315 (ATM)
K627 Ubiquitination
S630 Phosphorylation
K635 Ubiquitination
S661 Phosphorylation
K665 Acetylation
K665 Ubiquitination
S673 Phosphorylation
Y679 Phosphorylation
K683 Ubiquitination
K690 Acetylation
Y693 Phosphorylation
K698 Acetylation
S706 Phosphorylation
K715 Acetylation
K715 Methylation
K715 Ubiquitination
S738 Phosphorylation
K751 Methylation
K751 Ubiquitination

研究背景

功能:

Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.

翻译修饰:

Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.

细胞定位:

Nucleus. Nucleus>PML body. Chromosome>Telomere. Chromosome.
Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous. Expressed at high levels in testis.

亚基结构:

Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN. As part of the MRN complex, interacts with MCM9; the interaction recruits the complex to DNA repair sites. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN. Interacts with histone H2AX this requires phosphorylation of H2AX on 'Ser-139'. Interacts with HJURP. Interacts with INTS3. Interacts with KPNA2. Interacts with TERF2. Interacts with RBBP8; the interaction links the role of the MRN complex in DNA double-strand break sensing to resection. Interacts with SP100; recruits NBN to PML bodies. Interacts with ATF2. Interacts with MTOR, MAPKAP1 isoform 2 and RICTOR; indicative for an association with the mTORC2 complex. Interacts with MRNIP.

(Microbial infection) Interacts with herpes simplex virus 1 protein UL12.

蛋白家族:

The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.

The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.

The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.

研究领域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Genetic Information Processing > Replication and repair > Homologous recombination.

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.