FGFR3 Antibody - #AF0160

Figure 3 FOXG1 binds and regulates the expression of Fgfr3. (A) RNA-seq analysis of FACS-purified Control and Foxg1 loss-of-function (LOF) progenitors harvested two days after labelling at E15.5. Gliogenic factors such as Nfia, Id3, and Olig3 are upregulated, and neuronal markers such as Pou3f1 and Robo4 are downregulated. (B–D) Multimodal analysis comparing FOXG1 occupancy (ChIP-seq) and bivalent epigenetic marks (H3K4Me3 and H3K27Me3) and astrocyte-enriched genes from Telley et al., 2019 reveals a list of 19 genes common to each dataset (B). Four of these are upregulated upon loss of Foxg1, including the known gliogenic gene Fgfr3 (D). FOXG1 occupies a –26 kb enhancer region of Fgfr3 (C). (E) KEGG analysis of the upregulated genes from (A) identifies the MAPK signalling pathway downstream of FGF signalling. (F) Loss of Foxg1 from E15.5 progenitors (hGFAP-CreERT2, tamoxifen at E15.5) causes upregulation of FGFR3 receptor by E17.5 as seen in cells near the VZ of the somatosensory cortex. Boxes (F) indicate the regions in high magnification shown in the adjacent panels (G). Dashed circles outline the regions of interest (ROIs) identified in the DAPI channel used for intensity quantification in (H). (G; n = 50 [Control and Foxg1 LOF] ROIs from N = 3 brains) and phosphorylated-ERK1/2 (H; n = 67 [Control] and 89 [Foxg1 LOF]) cells from N = 3 brains (biologically independent replicates). Statistical test: Mann–Whitney test *p

Fig. 3. Effects of LRG on the gene expression in PA-treated LPS-induced RAW264.7 cells. (A) Effects of LRG on secretion of FGF21 in culture supernatant; (B) Effects of LRG on mRNA expression of FGF21; (C) Effects of LRG on mRNA expression of ACC1; (D) Effects of LRG on the protein expression of FGF21, p-FGFR2 and p-FGFR3; (E) Quantification of Fig. 3D; (F) Effect of LRG on protein expression of AMPK pathway; (G) Quantification of Fig. 3F. #p < 0.05, ##p < 0.01 vs CON; $p < 0.05, $$p < 0.01 vs LPS þ HGHP; &p < 0.05, &&p < 0.01 vs PA þ LPS þ LRG (L); *p < 0.05, **p < 0.01 vs PA þ LPS þ LRG (M).