TWIST1 Antibody - #BF0018
| 产品: | TWIST1 抗体 |
| 货号: | BF0018 |
| 描述: | Mouse monoclonal antibody to TWIST1 |
| 应用: | WB ELISA |
| 反应: | Human |
| 分子量: | 21kDa; 21kD(Calculated). |
| 蛋白号: | Q15672 |
| RRID: | AB_2834065 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0018, RRID:AB_2834065.
展开/折叠
ACS3; B-HLH DNA binding protein; bHLHa38; BPES2; BPES3; Class A basic helix-loop-helix protein 38; CRS; CRS1; CSO; H-twist; OTTHUMP00000116043; SCS; TWIST; Twist basic helix loop helix transcription factor 1; Twist family bHLH transcription factor 1; Twist homolog 1 (Drosophila); Twist homolog 1; TWIST homolog of drosophila; Twist related protein 1; Twist-related protein 1; TWIST1; TWST1_HUMAN;
抗原和靶标
Purified recombinant fragment of human TWIST1 expressed in E. Coli.
- Q15672 TWST1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMQDVSSSPVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGGGVGGGDEPGSPAQGKRGKKSAGCGGGGGAGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH
翻译修饰 - Q15672 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S18 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S20 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K38 | Ubiquitination | Uniprot | |
| S42 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S45 | Phosphorylation | Uniprot | |
| S68 | Phosphorylation | Q15759 (MAPK11) , P45983 (MAPK8) , Q16539 (MAPK14) , P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| K73 | Acetylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| Y103 | Phosphorylation | Uniprot | |
| T121 | Phosphorylation | Uniprot | |
| S123 | Phosphorylation | P31749 (AKT1) | Uniprot |
| T137 | Phosphorylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| K145 | Ubiquitination | Uniprot |
研究背景
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer (By similarity).
Nucleus.
Subset of mesodermal cells.
Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed (By similarity).
研究领域
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
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