CD31 Antibody - #AF0077

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产品: CD31 抗体
货号: AF0077
描述: Rabbit polyclonal antibody to CD31
应用: WB IHC IF/ICC
文献验证: IHC, IF/ICC
反应: Human
预测: Zebrafish
蛋白号: P16284
RRID: AB_2833268

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
CD31 Antibody detects endogenous levels of total CD31.
RRID:
AB_2833268
引用格式: Affinity Biosciences Cat# AF0077, RRID:AB_2833268.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Adhesion molecule; CD31; CD31 antigen; CD31 EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA; GPIIA'; PECA1; PECA1_HUMAN; Pecam 1; PECAM 1 CD31 EndoCAM; PECAM; PECAM-1; Pecam1; Platelet and endothelial cell adhesion molecule 1; Platelet endothelial cell adhesion molecule; Platelet/endothelial cell adhesion molecule 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human CD31, corresponding to a region within C-terminal amino acids.

基因/基因ID:
PECAM1(5175)
描述:
PECAM-1 a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). The direct binding of bioactive peptide Andersonin-W1 to TLR4 expedites the healing of diabetic skin wounds. Cellular & molecular biology letters, 2024 (PubMed: 38317065) [IF=9.2]
2). Freeze-drying prepared ready-to-use gelatin @polypropylene nonwoven hybrid sheet for stacking 3D cell culture. International Journal of Chronic Obstructive Pulmonary Disease, 2021 (PubMed: 34675506) [IF=4.9]

Application: IF/ICC    Species: Human    Sample: HUVEC

Cell-immunoassay on freeze-drying prepared gelatin @PP nonwoven sheet. a procedure of cell-immunoassay on ready-to-use gelatin @PP nonwoven sheet. b Photo of colorimetric changes from cell-immunoassay
3). SU5416 attenuated lipopolysaccharide-induced acute lung injury in mice by modulating properties of vascular endothelial cells. Drug Design Development and Therapy, 2019 (PubMed: 31213766) [IF=4.7]

Application: IHC    Species: mouse    Sample: alveola rendothelial cells

Figure 3 |TLR4/NF-κB signaling was involved in the progression of LPS-stimulated ALI. (A) Immunohistochemical staining of CD31 in LPS-stimulated WT and TLR4−/- mice after treatment with DXM and/or SU5416+DXM.
4). Tissue-resident memory CD103+CD8+ T cells in colorectal cancer: its implication as a prognostic and predictive liver metastasis biomarker. Cancer immunology, immunotherapy : CII, 2024 (PubMed: 38954030) [IF=4.6]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 3 The expression of CD31 and α-SMA in clinical samples and the anti-angiogenic therapy promotes infiltration of CD103+CD8+ TRMs in tumor. A The expression of CD31 and α-SMA in CRC and CRC liver metastasis tissue. B The infiltration level of CD103+CD8+ TRMs in the α-SMA high and low groups. C Comparison of median number and interquartile range of CD103+CD8+ TRMs. D The relationship of CD103+CD8+ TRMs and α-SMA+ vessel. E–F The infiltration level of CD103+CD8+ TRMs in CRC patients who responded and non-respond to bevacizumab treatment. G Average tumor growth curves showing tumor volume in mice treated with either control or the Bevacizumab (n = 5 mice per group). H–K Representative flow cytometry plots (H) and the percentage of CD103+CD8+ TRMs (I) and representative flow cytometry plots (J) and the percentage of IFN-γ+ CD103+CD8+ TRMs (K) isolated from mice on day 21 (n = 5 mice per group). (Immunofluorescent staining, × 400, The white triangle in the tissue is CD103+CD8+ TRMs). CRC, colorectal cancer; TRM, tissue-resident memory T cell. α-SMA, alpha-smooth muscle actin. *p 
5). Intracavernosal Adeno-Associated Virus-Mediated S100A1 Gene Transfer Enhances Erectile Function in Diabetic Rats by Promoting Cavernous Angiogenesis via VEGF-A/VEGFR2 Signaling. Journal of Sexual Medicine, 2019 (PubMed: 31378707) [IF=3.3]

Application: IF/ICC    Species: rat    Sample: endothelium in corpus cavernosum

Figure 5.| Effect of S100A1 overexpression on the Akt-eNOS signaling pathway and endothelial content. Panel A shows a representative Western blot of p-Akt, Akt, p-eNOS, and eNOS in each group. Panel B shows the relative density of total Akt and total eNOS compared with b-Actin; densitometric ratios of p-Akt to total Akt and p-eNOS to total eNOS. Panel C shows cavernous NO levels detected using a total NO assay kit. Panel D shows cavernous cGMP concentration measured with an enzyme-linked immunosorbent assay kit. Panel E shows endothelium in corpus cavernosum was detected with immunofluorescence using an antibody against CD31 (red, 100). Nuclei were stained by DAPI (blue).
6). Forkhead Box C2 Attenuates Lipopolysaccharide-Induced Cell Adhesion via Suppression of Intercellular Adhesion Molecule-1 Expression in Human Umbilical Vein Endothelial Cells. DNA and Cell Biology, 2019 (PubMed: 30994379) [IF=2.6]

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